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Identity, composition and abundance of protists on the BROKE-West voyage

Entry ID: BROKE-West_Protists

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Summary

Abstract: The BROKE-West survey was conducted from 30 degrees E and 80 degrees E between January and March 2006. It consisted of 1 east-west transect at the northernmost limit of the survey between 60 degrees S and 62 degrees S between the 10 and 19 of January, followed by 11 meridional transects separated by 5 degrees of longitude and extending from approximately 62 degrees S to the Antarctic continental ...

Related URL

Link: GET DATA

Description: Download point for the data

Geographic Coverage

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Spatial coordinates

	N: -59.78	S: -69.08	E: 80.0	W: 29.99

Min Depth: 4.6 M Max Depth: 102 M

Parent DIF

This data set description is a member of a collection. The collection is described in
BROKE-West

Temporal Coverage

Start Date: 2006-01-10

Stop Date: 2006-02-27

Location Keywords

CONTINENT > ANTARCTICA

OCEAN > SOUTHERN OCEAN

GEOGRAPHIC REGION > POLAR

Science Keywords

BIOLOGICAL CLASSIFICATION > PROTISTS

OCEANS > OCEAN CHEMISTRY > PIGMENTS > CHLOROPHYLL

OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL

ISO Topic Category

BIOTA

OCEANS

Platform

R/V AA > R/V Aurora Australis

Instrument

CTD > Conductivity, Temperature, Depth

NISKIN BOTTLES

SCANNING ELECTRON MICROSCOPES

TEM > TRANSMISSION ELECTRON MICROSCOPY

Quality Sea water samples for determining the identity, composition and abundance of protists were obtained using 20 l Niskin bottles (General Oceanics) mounted on a CTD rosette at 30 sites in surface waters (3-12 m depth) and 26 sites at the Chl max (11-80 m) over a total of 34 locations during the BROKE-West survey. Samples were preserved with 1% vol:vol Lugol's iodine and stored in glass bottles, in ... the dark at 4 degrees C until processing for either light microscopy or electron microscopy.

Permanent slides were prepared according to the 2-hydroxypropyl methacrylate (HPMA) (Sigma) method of Crumpton (1981), further modified by Sung-Ho Kang (KORDI, pers. comm.). Each sample was filtered at less than 5 kPa onto a 0.45 micron pore size, 25 mm diameter, GN-6 cellulosic membrane filter (Gelman). The filter was then rinsed by filtering a further 15 ml of MilliQ water, removed from the filtration apparatus and placed face down on a cover glass. A few drops of HPMA were placed on the back of the filter and the sample was then transferred to a 60 degrees C cabinet for 12 to 24 h to clear the filter and polymerise the HPMA. Further drops of HPMA were then placed on the back of the filter, a slide placed on top and the sample again polymerised as above for 6-12 h.

Identification of cryptic species was aided by electron microscopy. One litre samples were fixed with 1% glutaraldehyde and stored at 4 degrees C until processed. Each sample was concentrated over a 47 mm diameter 0.8 microns polycarbonate membrane (Poretics) at a vacuum of less than 50 mm Hg and the concentrate resuspended into the sea water remaining above the filter by gentle agitation. For scanning electron microscopy (SEM) cells were settled onto a polylysine-coated glass coverslips, dehydrated with a graded series of acetone, critical point dried, sputter coated with gold and examined using a JEOL JSM 840 SEM. For transmission microscopy (TEM), approximately 40 microlitre aliquots of concentrate were placed on polylysine treated formvar-coated copper grids (Marchant and Thomas, 1983) and fixed for 60 s with 2% OsO4 vapour. The aliquot was allowed to evaporate and the grid was gently rinsed with distilled water. The grids were either negative-stained with 2% uranyl acetate for 30 sec or shadow-cast with platinum/palladium or chromium and examined with a Phillips CM 100 TEM.

Prepared slides were examined using a Zeiss Axioskop microscope equipped with Nomarski interference optics at 400x magnification. Mean concentrations of each protistan taxa were calculated from the number of cells observed over 20 randomly selected quadrats (Whipple grids) for each sample.

Access Constraints These data are publicly available for download at the provided URL.

Use Constraints This data set conforms to the PICCCBY Attribution License
(http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-We... when using these data.

Ancillary Keywords

Protists

Autotrophs

Data Set Progress

COMPLETE

Data Center

Australian Antarctic Data Centre, Australia

Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC Phone: +61 3 6232 3244 Fax: +61 3 6232 3351 Email: metadata at aad.gov.au Contact Address: Australian Antarctic Division 203 Channel Highway City: Kingston Province or State: Tasmania Postal Code: 7050 Country: Australia

Distribution

Distribution Media: HTTP

Distribution Size: 21 kb

Distribution Format: Excel

Fees: free

Personnel

ANDREW TIMOTHY DAVIDSON

Role: INVESTIGATOR

Role: TECHNICAL CONTACT

Phone: +61 3 6232 3444

Fax: +61 6232 3351

Email: andrew.davidson at aad.gov.au

Contact Address:

Australian Antarctic Division

203 Channel Highway

City: Kingston

Province or State: Tasmania

Postal Code: 7050

Country: Australia

Creation and Review Dates

DIF Creation Date: 2009-08-25

Last DIF Revision Date: 2012-10-17

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