Using microsatellites as markers to explore genetic variation in Ceratodon purpureusEntry ID: ASAC_2542
Abstract: Sampling records for Project 2542 Moss sampling in the Windmill Islands and the Vestfold Hills during 2005 for genetic analysis. Samples are stored frozen at the University of Wollongong and will be analysed using microsatellites.
Collections of the mosses Ceratodon purpureus and Grimmia antarctici from the Windmill islands region and the Vestfold Hills. Sites include Clark Peninsula, Antarctic ... Specially Protected Areas 135 and 136 (formerly known as SSSI16 and SSSI 17), Robinson's Ridge, Peterson Island and around Casey station in the Windmill islands, and Mossel Lake, Lichen Valley and Grimmia Gorge in the Vestfold Hills.
Windmill Islands collections 11-14 January 2005.
Vestfold Hills collections 20-21 January 2005.
Samples were collected with tweezers, with each sample containing 5-10 shoots. Samples will be used to survey the genetic variation in these mosses in the Antarctic.
Notes about the dataset: Random, medium and fine refer to the scale of sampling used.
Random samples were a single sample (tweezer pinch) of moss was taken from that GPS point.
Medium scale samples used a 20 x 20 cm quadrat to collect samples at each of the corners and the centre of the quadrat, with a total of five samples for the GPS position.
For fine scale samples, a small clump of shoots, with approximately 2 cm square of shoot tips each, were collected, so once again were only a single collection per position, except for the fine scale sampling at Robinson's Ridge, in which 25 samples were taken within a 5 x 5 cm quadrat.
M8 marker etc. refer to various markers within or around the moss beds from previous or ongoing studies (by other researchers). These were recorded as additional guides for relocating sample sites if need be.
'Z pattern' refers to the order of sample collection within the quadrat.
The fields in this dataset are:
Two new spreadsheets were added in October, 2007. The following notes apply to these spreadsheets.
'metadata windmill is.xls'
'metadata all Ceratodon genotypes.csv'
Quadrat and independent labels are used in some instances instead of 'fine, medium and random' that have been previously used. Quadrat and independent are equivalent to the medium and random sampling in previous spreadsheets. Fine scale samples weren't genotyped.
Each of the five columns F-J (in both spreadsheets, in 'metadata windmill is.xls' it is the 'genotypes' sheet) are a different microsatellite locus for the moss Ceratodon purpureus (e.g. CEPU105), with the numbers in each row indicating the microsatellite allele or genotype as the length in DNA base pairs in that sample.
Multilocus genotype ('metadata windmill is.xls' spreadsheet, 'genotypes and GPS' worksheet) is the combination of each of the 5 microsatellite alleles, if two samples share the same 5 alleles they have the same multilocus genotype. The GenAlEx program (this is the input for a population genetics program) gives a letter for genotypes present in more than one sample, and a number for genotypes only present in one sample, so 'E' is a very common multilocus genotype in this data set.
In February 2011, two more documents were added. A csv spreadsheet detailing locations of mosses found in 2005, and a dating word document.
Start Date: 2005-01-11Stop Date: 2005-01-14
Start Date: 2005-01-20Stop Date: 2005-01-21
ISO Topic Category
Quality The latitudes and longitudes provided in spatial coverage are approximate only.
Access Constraints The data are publicly available for download from the URL given below or in the data centre database or GBIF.
The dating data, however, are not yet publicly available.
Use Constraints This data set conforms to the PICCCBY Attribution License
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2542 when using these data.
Data Set Progress
Distribution Media: HTTP
Distribution Size: 21 kb
Distribution Format: csv, excel
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Phone: +61 2 4221 5753
Fax: +61 2 4221 4135
Email: sharonr at uow.edu.au
Department of Biological Sciences University of Wollongong Northfields Ave
Province or State: New South Wales
Postal Code: 2522
Dunn J.L., Robinson S.A. (2006), Ultraviolet B screening potential is higher in two cosmopolitan moss species than in a co-occurring Antarctic endemic moss: implications of continuing ozone depletion., Global Change Biology, 12, 2282-2296
Hennion F., Huiskes A.H.L., Robinson S., Convey P. (2006), Physiological traits of organisms in a changing environment., Bergstrom D.M., Convey P., Huiskes A.H.L. Trends in Antarctic Terrestrial and Limnetic Ecosystems: Antarctica as a Global Indicator., 127-157
Dunn J.L., Robinson S.A. (2006), UV-B screening potential is higher in two cosmopolitan moss species than in a co-occurring Antarctic endemic moss - implications of continuing ozone depletion, Global Change Biology, 12, 12, 42
Clarke, LJ, Robinson, SA and Ayre, DJ (2009), Genetic structure of Antarctic populations of the moss Ceratodon purpureus., Antarctic Science, 21, 51-58
Clarke, LJ, Robinson, SA and Ayre, DJ (2008), Somatic mutation and the Antarctic ozone hole, Journal of Ecology, 96, 378-385
Extended Metadata Properties
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Creation and Review Dates
DIF Creation Date: 2005-03-02
Last DIF Revision Date: 2014-08-29