Molecular analysis of microbiota trapped in ancient antarctic glacial ice
Entry ID:
ASAC_2763
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Summary
Abstract:
Metadata record for data from ASAC Project 2763 See the link below for public details on this project. Ancient Antarctic glacial ice is a potential resource of trapped microorganisms dating back several hundreds of thousand years that give a snapshot of the past. Nucleic acid, such as DNA, has been identified in samples as old as these from Bacteria, Archaea and Viruses, and this will be the ... focus of this study. Outcomes of this research will determine the type of organisms that become trapped in these ancient samples, and whether they are able to survive such an extreme condition, and may even lead to novel species being discovered, or even new genes and products. Project objectives: Determine the type of microorganisms that become trapped in ancient Antarctic glacial ice and ascertain whether glacial ice in Antarctica harbours biota that are of evolutionary or biotechnological interest. Public summary of the season progress: Ancient glacial ice samples were collected from ice cliff areas located near Casey Station during the just recent Summer expedition. Ice samples were transported back to Australia and will be subject to 454 sequencing analysis in the next few months in collaboration with Prof. Alan Cooper, Centre for Ancient DNA, University of Adelaide. Other work being performed was completed included an initial molecular-based microbial survey of unusual alkaline, permanently ice-covered, continental lakes located in the Framnes Mountains, inland from Mawson The first download file contains: 10 files File 1. Chemical and oxygen isotope data for Law Dome ice cores that were used in microbiological studies (isolation and DNA analyses). File 2. Chemical and oxygen isotope data for an Amery Ice Shelf ice core that was used in microbiological studies (isolation and DNA analyses). File 3. 16S rRNA gene sequence data obtained from Law Dome ice core samples. All sequences are clones derived after direct PCR amplification of DNA extracts, no isolates were obtained. File 4. 16S rRNA gene sequence data obtained from Amery Ice Shelf ice core sample. All sequences are clones derived after direct PCR amplification of DNA extracts, no isolates were definitively obtained. File 5. Soil and similar samples obtained from either the Vestfold Hills, Eastern Antarctica or from Macquarie Harbour. These soils are the source of actinobacteria screened in the project for antimicrobial activity. File 6. Actinobacteria/actinomycete isolates information detailing isolation procedure, colonial/cellular characteristics, and tentative identification. Barcode system was used to track isolates. Represents the "(University of Tasmania Antarctic Actinobacteria) UTAA" collection. File 7. Preliminary antimicrobial screening trial data for 1267 Antarctic actinobacterial isolates against 5 strains of Listeria monocytogenes. A secondary screen was performed to identify those with reliable bioactivity. File 8. Broader analysis of antimicrobial activities of Antarctic isolates against a panel of bacterial pathogenic bacteria. All strains were identified to species level within the genus Streptomyces. File 9. Hydrocarbon profiles of selected Antarctic actinobacterial strains against a range of aliphatic and polycyclic hydrocarbons. File 10. Phenotypic, chemotaxonomic and identification (by 16S rRNA gene sequencing) data of Antarctic hydrocarbon degrading strains. The second download file contains: 3 files: File 1. Framnes Mountain epiglacial lake sample data. Indicates lakes sampled, location, chemical data (pH, temperature). File 2. Cloned 16S rRNA gene sequences obtained from Patterned Lake water DNA extracts. All sequnces are in FASTA format. File 3. Cloned 16S rRNA gene sequences obtained from Sonic Lake water DNA extracts. All sequnces are in FASTA format.
Related URL
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Description:
Download point for the data - part 1
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Geographic Coverage
(Click for Interactive Map)
Spatial coordinates
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N: -66.0
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S: -67.0
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E: 111.0
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W: 110.0
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Temporal Coverage
Start Date:
2006-10-01
Stop Date:
2007-03-31
Quality
The Dates provided in temporal coverage are approximate only, and represent the beginning and end of the 2006 - 2007 Antarctic seasons. The latitudes and longitudes provided in spatial coverage are approximate only.
Access Constraints
The data are publicly available for download from the provided URLs.
Data Set Progress
COMPLETE
Distribution
Distribution Media:
HTTP
Distribution Size:
56 kb
Distribution Format:
csv, text
Fees:
Free
Distribution Media:
HTTP
Distribution Size:
7 kb
Distribution Format:
csv, text
Fees:
Free
Personnel
Role:
TECHNICAL CONTACT
Phone:
+1 (303) 492-6199
Fax:
+1 (303) 492-2468
Email:
nsidc at nsidc.org
Contact Address:
National Snow and Ice Data Center
CIRES, 449 UCB
University of Colorado
City:
Boulder
Province or State:
CO
Postal Code:
80309-0449
Country:
USA
Role:
TECHNICAL CONTACT
Email:
David.W.Hancock at nasa.gov
Contact Address:
ISIPS Manager
NASA Goddard Space Flight Center
Wallops Flight Facility
USA
City:
Wallops Island
Province or State:
VA
Country:
USA
Role:
INVESTIGATOR
Phone:
301-614-5643
Fax:
301-614-5644
Email:
Jay.Zwally at nasa.gov
Contact Address:
Laboratory for Hydrospheric Processes
NASA/Goddard Space Flight Center
Code 614
City:
Greenbelt
Province or State:
MD
Postal Code:
20771
Country:
USA
Role:
INVESTIGATOR
Phone:
512-471-1356
Fax:
512-471-3570
Email:
schutz at utcsr.ae.utexas.edu
Contact Address:
GLAS Science Team Lead
University of Texas at Austin
Center for Space Research
City:
Austin
Province or State:
TX
Postal Code:
78712-1085
Country:
USA
Role:
INVESTIGATOR
Contact Address:
Dept. of Geology and Geophysics
University of Wisconsin
City:
Madison
Province or State:
WI
Postal Code:
53706
Country:
USA
Role:
INVESTIGATOR
Contact Address:
NASA/Goddard Space Flight Center
City:
Greenbelt
Province or State:
MD
Postal Code:
20771
Country:
USA
Role:
INVESTIGATOR
Contact Address:
Massachusetts Institute of Technology
City:
Cambridge
Province or State:
MA
Postal Code:
02139
Country:
USA
Role:
INVESTIGATOR
Contact Address:
Scripps Institute of Oceanography
University of California
City:
La Jolla
Province or State:
CA
Postal Code:
92093
Country:
USA
Role:
INVESTIGATOR
Phone:
301-614-6274
Email:
James.D.Spinhirne at nasa.gov
Contact Address:
NASA Goddard Space Flight Center
Mailstop 613.1
City:
Greenbelt
Province or State:
MD
Postal Code:
20771
Country:
USA
Role:
INVESTIGATOR
Contact Address:
EG&G
NASA Wallops Flight Facility
City:
Wallops Island
Province or State:
VA
Postal Code:
23337
Country:
USA
Publications/References
Bae, S. and B.E. Schutz. 2002. Precision attitude determination (PAD). GLAS algorithm theoretical basis document. Version 2.2. Austin, TX: Center for Space Research, University of Texas at Austin. Brenner, A.C., C.R. Bentley, B.M. Csatho, D.J. Harding, M.A. Hofton, J. Minster, L. Roberts, J.L. Saba, R. Schutz, R.H. Thomas, D. Yi, and H.J. Zwally. 2000. Derivation of range and range distributions ... from laser pulse waveform analysis for surface elevations, roughness, slope, and vegetation heights. Algorithm theoretical basis document. Version 3.0. Greenbelt, MD: Goddard Space Flight Center. Center for Space Research. 'ICESat/GLAS.' January 2002. http://www.csr.utexas.edu/glas/. Davis C.H. and H.J. Zwally. 1993. Geographic and seasonal variations in the surface properties of the ice sheets by satellite radar altimetry. Journal of Glaciology 39:687-697. Earth Science Data and Information System (ESDIS). 1996. EOS Ground System (EGS) Systems and Operations Concept. Greenbelt, MD: Goddard Space Flight Center. Farrell, W.E. 1972. Deformation of the Earth by surface loads. Review of Geophysics and Space Physics 10: 761-797. GLAS Science Team. 2000. Mission Operations Requirement Document (MORD) for the Ice, Cloud and Land Elevation Satellite (ICESat) Mission. Greenbelt, MD: Goddard Space Flight Center. GLAS Science Team. 1997. GLAS Science Requirements. Version 2.01. Greenbelt, MD: Goddard Space Flight Center. Heroux, D. 2000. Progress report on GLAS interface effort with ECS NOSE. Rev. 29 December 2000. Landover, MD: Emergent-IT/Raytheon. Herring, T.A. and K. Quinn. 1999. Atmospheric delay correction to GLAS laser altimeter ranges. GLAS algorithm theoretical basis document. Version 1.0. Lanham, MD: Science Systems and Applications, Inc. Jester, P., and D. Hancock. 2002. The Algorithm Theoretical Basis Document for Level 1A Processing, Version 1.2. Wallops Island, VA: Raytheon ITSS. Jester, P., and J. Lee. 2002. GLAS standard data products specification - level 1. Version 6.0. Wallops Island, VA: Raytheon ITSS. Lee, J. 2002. GSAS user's guide: Version 4.0. Wallops Island, VA: Raytheon ITSS. McGarry, J.F., J. Abshire, X. Sun, J. Saba, A. Brenner, and D. Yi. 2002. GLAS flight science data selection algorithms for the altimeter (1064 nm), Version 4.15. Unpublished report. Rim, H., and B. Schutz. 2002. Precision orbit determination. GLAS algorithm theoretical basis document. Version 2.2. Austin, TX: Center for Space Research, University of Texas at Austin. Schutz, B. 2000. GLAS altimeter post-launch calibration/validation plan. Version 0.99. Austin, TX: Center for Space Research, University of Texas at Austin. Schutz, B. 2002. Laser footprint location (geolocation) and surface profiles. GLAS algorithm theoretical basis document. Version 3.0. Austin, TX: Center for Space Research, University of Texas at Austin. Wahr, J., D. Wingham, and C. Bentley. 2000. A method of combining ICESat and GRACE satellite data to constrain Antarctic mass balance. Journal of Geophysical Research 105(B7):16,279-16,294. Yi, D., J. Minster, and C. Bentley. 1999. Ocean tidal loading corrections. GLAS algorithm theoretical basis document. Version 1.0. Greenbelt, MD: Goddard Space Flight Center. Zwally, H.J., B. Schutz, W. Abdalati, J. Abshire, C. Bentley, A. Brenner, J. Bufton, J. Dezio, D. Hancock, D. Harding, T. Herring, B. Minster, K. Quinn, S. Palm, J. Spinhirne, and R. Thomas. 2002. ICESAT's laser measurements of polar ice, atmosphere, ocean, and land. Journal of Geodynamics 34: 405-445. Zwally, H.J., B. Schutz, D. Hancock, and A. Brenner. 2000. ICESat/GLAS Standard Data Products in HDF and SCF Formats. Version 1.2. Greenbelt, MD: Goddard Space Flight Center. Zwally, H.J., R.A. Bindschadler, A.C. Brenner, T.V. Martin, and R.H. Thomas. 1983. Surface elevation contours of Greenland and Antarctic ice sheets. Journal of Geophysical Research 88(C3):1589-1596.
Extended Metadata Properties
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Creation and Review Dates
DIF Creation Date:
2002-10-28
Last DIF Revision Date:
2011-02-11
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