[Location: Location_Category='CONTINENT', Location_Type='ANTARCTICA', Detailed_Location='Vestfold Hills']
Molecular Taxonomy of an Antarctic, Mycoplasma-like Organism: Sequence of the 16S Ribosomal RNA GeneEntry ID: ASAC_504
Abstract: An obligately anaerobic bacterium that lacked a cell wall was isolated from the hypolimnion of Ace Lake, Antarctica. Cells were very pleomorphic, forming cocci, filaments up to 25 micrometres in length, and annular shapes. The organism was morphologically very similar to some members of the class Mollicutes which contains two genera of obligately anaerobic bacteria, Anaeroplasma and ... Asteroleplasma. Like members of the class, the isolate was resistant to high concentrations of penicillin (1000 Units per millilitre). Similar to Anaeroplasma, the organism had a low DNA G+C content (29.3 +/- 0.4) and produced hydrogen, carbon dioxide, acetic acid, lactic acid and succinic acid from the fermentation of glucose. However, the taxonomic status of the strain remained unclear as, unlike members of the class Mollicutes, the isolate had a relatively large genome size (2.26 +/- 0.11 x 10 to the 9 daltons), did not pass through 0.45 micrometre pore size filters, and did not form typical mycoplasma-like colonies. The organism was psychrophilic with an optimum temperature for growth between 12 degrees C and 13 degrees C. A phenotypic description of the organism is given and the ecological role of the organism is inferred from its phenotype and the characteristics of its Antarctic habitat.
Start Date: 1991-09-30Stop Date: 1992-03-31
Quality Values provided in temporal coverage are approximate only.
Access Constraints A pdf copy of one of the papers arising from this project is available for download for AAD staff from the provided URL.
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Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_504 when using these data.
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Distribution Size: 2698 kb
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Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Phone: +61 8 9333 6306
Fax: +61 8 9387 8211
Email: peter.franzmann at csiro.au
CSIRO LAND AND WATER PO WEMBLEY PRIVATE BAG
Province or State: Western Australia
Postal Code: 6014
Franzmann, P.D. and M. Rohde (1992), Characteristics of a novel, anaerobic, mycoplasma-like bacterium from Ace Lake, Antarctica, Antarctic Science, 4, 155-162
Franzmann, P.D. and Dobson, S.J. (1992), Cell wall-less, free-living spirochetes in Antarctica., FEMS Microbiology Letters, 97, 289-292
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Creation and Review Dates
DIF Creation Date: 2000-07-31
Last DIF Revision Date: 2014-01-10