[Parameters: Topic='BIOSPHERE', Term='ECOLOGICAL DYNAMICS', Variable_Level_1='ECOTOXICOLOGY']
Ecological risks from oil products used in Antarctica: characterising hydrocarbon behaviour and assessing toxicity on sensitive early life stages of Antarctic marine invertebrates - bioassays of marine invertebratesEntry ID: AAS_3054_bioassays_invertcontam
Abstract: This metadata record will contain the results of bioassays conducted to characterise the response of Antarctic nearshore marine invertebrates to hydrocarbon contaminants in fuels commonly used in Antarctica. AAS Project 3054.The results of Season 2 and Season 3 amphipod tests are in this dataset.
The three fuel types used in this project were: Special Antarctic Blend diesel (SAB), Marine Gas Oil ... diesel (MGO) and an intermediate grade (180) of marine bunker Fuel Oil (IFO). Fuels were experimentally mixed by slow stir of fuel and seawater in temperature control cabinets at -1C to prepare a mixture of fuel hydrocarbons in filtered seawater (FSW) termed the Water Accommodated Fraction (WAF). WAF was produced by adding fuel to seawater in 5 L or 10 L Pyrex glass bottles using a ratio of 1:24 Fuel : FSW. This mixture was stirred at slow speed with minimal vortex for 18 h on a magnetic stirrer. The mixture was settled for 6 h before the water portion was drawn from beneath the fuel.
Ecotoxicological bioassays were conducted at Davis and Casey Stations in 2010/11 and 2011/12 summer seasons using SAB WAF, MGO WAF and IFO WAF (specified above) to prepare experimental treatments consisting of WAF dilution series. For each bioassay, treatments consisted of undiluted 100% WAF and dilutions of 10% and 1% of WAFs in FSW, to test the toxicity of water accommodated fractions of these three fuels on Antarctic marine invertebrates. The majority of experiments tested WAFs of each of the three fuels, although one tested SAB only due to limited supply of test organisms.
Bioassays were conducted in open vessels (glass jars or beakers) in temperature controlled cabinets.
Mortality and/or sub-lethal effects were observed at endpoints of 24 hrs, 48 hrs, 96 hrs, 7 days, 8 days, 10 days, 12 days, 14 days, 16 days and 21 days. New WAF solutions were prepared at 4 day intervals to replenish the experimental treatments. Deionised water was added to test solutions as required to maintain test solution volume and salinity. Water quality data was collected at each water change. Samples of test treatments for chemical analysis of hydrocarbon concentration were taken at each water change. Results of these analyses are not included as delayed progress with HC analyses impacted on quality of samples and these data were not used.
This dataset consists of Excel spreadsheets. The file name code for invertebrate bioassays is;
Project number_Season_Taxa_Test name
Project number : AAS_3054
Season : 2010/11 season
Test name:2 for Season 2, PW for genus and species, A for adult, 1 for Test 1
Bioassay spreadsheets contain the results of bioassays for a species. Where replicate tests were conducted, each experiment is on a separate worksheet. The worksheet labelled "Test conditions" shows details of Test name, dates, animal collection details, laboratory holding conditions, details of water accommodated fractions (WAF), bioassay conditions, scoring criteria and water quality data. The worksheet labelled "Counts" has columns for Replicate number and columns with the Score for all the animals in that replicate at every time endpoint. A full description of the scoring criteria is on the "Test conditions" worksheet. Totals, means and standard deviations are calculated for each treatment. The worksheet labelled "Totals, means, percent, StDev" has calculations of Survival, Unaffected, including mean and standard deviation, Percent Survival and Unaffected including means and standard deviation. Also included is column for the Total number of moults in each treatment.
During the research to obtain early life stages of invertebrates for experiments, the number of Paramoera walkeri amphipod neonates per female, the timing of their release from the brood pouch and their early growth rate were recorded. These data are also included in AAS_3054_10_11_PW_neonates
Samples were collected from:
Ellis Narrows, Vestfold Hills
Airport Beach, Davis, Vestfold Hills
Prydz Bay, Davis (Between Anchorage Island and Bluff Island)
Bailey Peninsula, Windmill Islands
Data Set Citation
Dataset Originator/Creator: Harrison, P., King, C. and Brown, K.E.
Dataset Title: Ecological risks from oil products used in Antarctica: characterising hydrocarbon behaviour and assessing toxicity on sensitive early life stages of Antarctic marine invertebrates - bioassays of marine invertebrates
Dataset Series Name: CAASM Metadata
Dataset Publisher: Australian Antarctic Data CentreOnline Resource: https://data.aad.gov.au/aadc/metadata/metadata_redirect.cfm?md=/AMD...
Start Date: 2010-12-02Stop Date: 2011-03-23
Start Date: 2012-01-08Stop Date: 2012-02-05
Quality Problem in 1PWA1 with cabinet temperature running too low and icing up jars meant test conditions not conducive for animals and test aborted at 7 days. Endpoints from 96 hours not reliable.
Access Constraints These data are not yet publicly available.
Use Constraints This data set conforms to the PICCCBY Attribution License
Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_3054... when using these data.
Data Set Progress
Distribution Media: HTTP
Distribution Size: 741 kb
Distribution Format: Excel, Word
Role: TECHNICAL CONTACT
Phone: +61 2 6620 3774
Email: peter.harrison at scu.edu.au
Southern Cross University
Province or State: New South Wales
Postal Code: 2480
Role: TECHNICAL CONTACT
Phone: +61 3 6232 3515
Email: cath.king at aad.gov.au
Australian Antarctic Division 203 Channel Highway
Province or State: Tasmania
Postal Code: 7050
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Email: k.brown.14 at student.scu.edu.au
Marine Ecology Research Centre Southern Cross University PO Box 157
Province or State: NSW
Postal Code: 2480
Brown, K., King, C., Lane, A., Kotzakoulakis, K., George, S., and Harrison, P. (2013), Sensitivity of the Antarctic amphipod Paramoera walkeri to diesel and fuel oil: Lethal and behavioural impacts., Strategic Science in Antarctica Conference, Hobart, June, 2013.
Brown, K., King, C., George, S., Lane, A. and Harrison, P. (2012), Assessing toxicity of diesel and fuel oil on early life stages of Antarctic marine invertebrates., Proceedings of the 2nd SETAC Australasia Conference. Brisbane, July 2012, Abstracts p. 85.
Brown, K., King, C., George, S., Lane, A. and Harrison, P. (2012), Assessing toxicity of diesel and fuel oil on early life stages of Antarctic marine invertebrates., Open Science Conference of the Scientific Committee on Antarctic Research. Portland, Oregon, USA, July 2012.
Extended Metadata Properties
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Creation and Review Dates
DIF Creation Date: 2013-07-19
Last DIF Revision Date: 2014-08-27