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Real-time PCR measurements of alkB and rpoB in SAB contaminated sediment (SRE4 experiment).
Entry ID: SRE4_alkB_T1T2

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Abstract: Real-time PCR assays were used to measure the proportion of the microbial population that had the potential to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by developing assays to measure the levels of alkane mono-oxygenase genes (alkB) relative to ribosomal polymerase B sub-unit (rpoB) genes in soil and sediment samples. In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from the SRE4 in-situ biodegradation experiment.

The samples used in this experiment were collected from O'Brien Bay, near Casey Station in the Windmill Islands.

An information sheet is provided at the beginning of the excel workbook.

This work was completed as part of ASAC project 2672 (ASAC_2672).
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Geographic Coverage
Spatial coordinates   
  N: -66.0   S: -66.0   E: 110.0   W: 110.0

Parent DIF
This description is a member of a collection. The collection is described in

Temporal Coverage
Start Date: 2001-10-01
Stop Date: 2001-10-31

Start Date: 2002-12-01
Stop Date: 2002-12-31
ISO Topic Category
Quality The dates provided in temporal coverage are approximate only. Year and month are correct.
Access Constraints These data are not yet publicly available.
Use Constraints This data set conforms to the PICCCBY Attribution License

Please follow instructions listed in the citation reference provided at when using these data.
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Australian Antarctic Data Centre, Australia Supplemental Info
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Data Center Personnel
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia
Distribution Media: HTTP
Distribution Size: 1,332 kb
Distribution Format: Excel
Fees: free
Phone: +61 3 6226 6380
Fax: +61 3 6226 2642
Email: john.bowman at
Contact Address:
School of Agricultural Science
University of Tasmania
Private Bag 54
City: Hobart
Province or State: Tasmania
Postal Code: 7001
Country: Australia
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Creation and Review Dates
DIF Creation Date: 2006-04-07
Last DIF Revision Date: 2016-01-27

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