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Real-time PCR measurements of alkB in SAB contaminated sediment (SRE4 experiment).

Entry ID: SRE4_alkB_allyears

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Summary

Abstract: Real-time PCR assays were used to measure the potential of the microbial population to degrade alkanes - the principal component of Special Antarctic Blend diesel. This was done by using a real-time PCR assay to measure the levels of alkane mono-oxygenase genes (alkB). In this work, the assays were used to determine the levels of these genes in sediment exposed to SAB and control sediments from ...

Related URL

Link: GET DATA

Description: Download point for the dataset

Geographic Coverage

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Spatial coordinates

N: -66.0

S: -66.5

E: 110.5

W: 110.0

Parent DIF

This data set description is a member of a collection. The collection is described in
SRE4_Microbial

Temporal Coverage

Start Date: 2001-10-01

Stop Date: 2006-12-20

Location Keywords

CONTINENT > ANTARCTICA

GEOGRAPHIC REGION > POLAR

Science Keywords

HUMAN DIMENSIONS > ENVIRONMENTAL IMPACTS > OIL SPILLS

ISO Topic Category

ENVIRONMENT

Quality The dates provided in temporal coverage are a range. The specific dates of sample collection are given in the datafile.

Access Constraints These data can be downloaded from the provided URL.

Use Constraints This data set conforms to the PICCCBY Attribution License
(http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SRE4_alk... when using these data.

Ancillary Keywords

quantitative real-time PCR

alkane mono-oxygenase

special Antarctic blend diesel

Data Set Progress

COMPLETE

Data Center

Australian Antarctic Data Centre, Australia

Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC Phone: +61 3 6232 3244 Fax: +61 3 6232 3351 Email: metadata at aad.gov.au Contact Address: Australian Antarctic Division 203 Channel Highway City: Kingston Province or State: Tasmania Postal Code: 7050 Country: Australia

Distribution

Distribution Media: HTTP

Distribution Size: 12 kb

Distribution Format: Excel, Text, CSV

Fees: Free

Personnel

JOHN BOWMAN

Role: INVESTIGATOR

Phone: +61 3 6226 6380

Fax: +61 3 6226 2642

Email: john.bowman at utas.edu.au

Contact Address:

School of Agricultural Science

University of Tasmania

Private Bag 54

City: Hobart

Province or State: Tasmania

Postal Code: 7001

Country: Australia

SHANE POWELL

Role: TECHNICAL CONTACT

Role: DIF AUTHOR

Phone: +61 3 6226 6286

Fax: +61 3 6226 2642

Email: shanep at utas.edu.au

Contact Address:

Tasmanian Institute of Agricultural Research

University of Tasmania

Private Bag 54

City: Hobart

Province or State: Tasmania

Postal Code: 7001

Country: Australia

Publications/References

Powell S.M., Stark J.S., Snape I., Woolfenden E., Bowman J.P., Riddle M.J. (2010), Diesel and lubricant oils impact on Antarctic benthic microbial communities., Aquatic Microbiology Ecology, 61, 119-127

Thompson BAW, Goldsworthy PM, Riddle MJ, Snape I, Stark JS (2007), Contamination effects by a Conventional and a Biodegradable lubricant oil on infaunal recruitment to Antarctic sediments: a field experiment., Journal of Experimental Marine Biology and Ecology, 340, 213-26

Creation and Review Dates

DIF Creation Date: 2008-04-06

Last DIF Revision Date: 2012-04-04

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