Sequencing of the metallothionein gene using the RT-PCR (reverse transcriptase polymerase chain reaction) method in the Antarctic fish Pagothenia borchgrevinki to determine the exposure of Antarctic fish to heavy metals
Entry ID: K014_1997_1999_NZ_1

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Summary
Abstract: The development and application of a new molecular approach for quantifying the exposure of Antarctic fish to heavy metals was tested. The approach uses quantitative RT-PCR (reverse transcriptase polymerase chain reaction) to measure the extent of induction of the metallothionein gene which is sensitive to the presence of environmental metals such as cadmium and copper. The Antarctic fish species ... View entire text
Related URL
Description: Investigators home page
Geographic Coverage
Spatial coordinates   
  N: -77.57   S: -77.57   E: 166.2   W: 166.2
  N: -77.85   S: -77.85   E: 166.67   W: 166.67

Temporal Coverage
Start Date: 1997-12-01
Stop Date: 1997-12-09
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ISO Topic Category
Data Set Progress
COMPLETE
Data Center
Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand Supplemental Info
Data Center URL: http://www.antarcticanz.govt.nz/

Data Center Personnel
Name: SHULAMIT GORDON
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand
Personnel
Role: INVESTIGATOR
Phone: +64 9 373 7599 xtn 87245
Fax: +64 9 373 7415
Email: c.evans at auckland.ac.nz
Contact Address:
School of Biological Sciences
Molecular, Cellular and Developmental Biology Section
University of Auckland
Private Bag 92019
City: Auckland
Postal Code: 1142
Country: New Zealand
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Publications/References
Evans, CW, Wilson, DA and Mills, GN (2001): Quantitative competitive RT-PCR as a tool in biomarker analysis. Biomarkers 6: 7-14.
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Extended Metadata Properties
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Creation and Review Dates
DIF Creation Date: 2008-10-17
Last DIF Revision Date: 2008-11-05
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