Application of Enzyme-Linked Immunosorbent Assay for Measurement of Polychlorinated Biphenyls from Hydrophobic Solutions.
Entry ID: usgsbrdcerc_immunosorbentassay

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Summary
Abstract: The objectives of this work were 1) to study the recovery and homogenity of
polychlorinated biphenyls (PCBs) in solution prepared for ELISA by direct
dilution of isooctane solutions; 2) to evaluate nitrogen evaporation as a quick
and efficient means for solvent removal; 3) to examine the efficacy of methanol
and aqueous/methanol mixtures for quantitative recovery of residual PCBs,
following evaporation of the hydrophobic solvent; and 4) to compare
concentrations of ePCBs with their corresponding concentrations determined by
gas chromatography (GC) in selected fish extracts and in dialysates of
semipermeable membrane devices (SPMDs) used to monitor laboratory air.
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Description: Metadata On CERC's NBII Metadata Node

Data Set Citation
Dataset Originator/Creator: James L. Zajicek et al, U.S. Geological Survey, Biological Resources Division, Columbia Environmental Research Center (formerly National Biological Service)
Dataset Title: Application of Enzyme-Linked Immunosorbent Assay for Measurement of Polychlorinated Biphenyls from Hydrophobic Solutions.
Dataset Release Date: 1996
Dataset Release Place: Columbia, Missouri
Dataset Publisher: USGS, BRD, Columbia Environmental Research Center
Online Resource: http://www.cerc.usgs.gov/clearinghouse/data/usgs_brd_cerc_b_immunoa...
Access Constraints None
Use Constraints None
Data Set Progress
COMPLETE
Data Center
Columbia Environmental Research Center, Biological Resources Division, U.S. Geological Survey, U.S. Department of the Interior Supplemental Info
Data Center URL: http://www.cerc.usgs.gov/

Data Center Personnel
Name: CHRIS HENKE
Phone: (573) 875-5399
Fax: (573) 876-1863
Email: chris_henke at usgs.gov
Contact Address:
U.S. Geological Survey
Biological Resources Division
Columbia Environmental Research Center
4200 New Haven Road
City: Columbia
Province or State: MO
Postal Code: 65201-9634
Country: USA
Personnel
Role: DIF AUTHOR
Phone: (301) 614-6898
Fax: 301-614-5268
Email: Tyler.B.Stevens at nasa.gov
Contact Address:
NASA Goddard Space Flight Center
Global Change Master Directory
City: Greenbelt
Province or State: MD
Postal Code: 20771
Country: USA
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Publications/References
James L. Zajicek, Donald E. Tillitt, James N. Huckins, Jimmie
D. Petty, Michael E. Potts, and U.S. Geological Survey, Biological
Resources Division, Columbia Environmental Science Center (formerly
Midwest Science Center) David A. Nardone, Ohmicron Corporation
1996. Application of Enzyme-Linked Immunosorbent Assay for Measurement
of Polychlorinated Biphenyls from Hydrophobic Solutions. American
Chemical Society.
Extended Metadata Properties
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Creation and Review Dates
DIF Creation Date: 2000-06-01
Last DIF Revision Date: 2013-10-18
Future DIF Review Date: 2001-06-01
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