Effects of increased UVB radiation on Antarctic marine microalgaeEntry ID: NL_ANTARCTIC_UVB_MICROALGAE
Abstract: NATURAL UVBR CAUSES DNA DAMAGE IN ANTARCTIC MARINE PHYTOPLANKTON
ANITA G.J. BUMA, M. K. DE BOER, W.W.C. Gieskes
UVBR stress in Antarctic marine phytoplankton was studied under
natural irradiance conditions. During a research period of 3 months at
Rothera Station (67S, British Antarctic Survey) data were collected
regarding UVBR penetration, using a biochemical dosimeter, based on
... the induction of DNA damage (cyclobutane pyrimidine dimers, CPD's) in
bare DNA. Furthermore, UVBR induced DNA damage was studied in several
size fractions of natural phytoplankton populations. Finally,
incubation experiments were conducted to study wavelength dependent
rates of DNA damage induction as well as repair.
The results show that in the Rothera Bay area attenuation of
biologically effective UVBR is fast, likely due to the presence of
phytoplankton (mainly diatom) blooms, giving 1 BED (Biologically
Effective Dose) levels between 5 and 8 meters. In most cases, damage
levels decreased with depth in all size fractions. The smallest size
fraction (0.2 - 2 mm) gave the highest damage levels in all cases.
CPD's were solely induced by UVBR, and exposure to natural solar
radiation induced several tens of CPD's (per million nucleotides)
within hours, mainly in the smallest size fraction. Repair of damage
was visible when populations were exposed to UVAR + PAR, but not with
PAR alone, indicating that highest photolyase activity is taking place
in the mid UVAR region.
The results clearly show that phyto- and bacterioplankton from marine
Antarctic systems suffer from UVBR stress under natural, non-ozone
depleted conditions. It also indicates that the smallest organisms,
i.e. bacteria are more vulnerable for DNA damage induction than larger
phytoplankton cells, as found elsewhere. It is presently not known how
these differences in vulnerability affect food web dynamics in marine
(Click for Interactive Map)
Start Date: 1998-01-17Stop Date: 1998-03-05
OCEANS > SALINITY/DENSITY > SALINITY
SPECTRAL/ENGINEERING > ULTRAVIOLET WAVELENGTHS > ULTRAVIOLET FLUX
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > AQUATIC ECOSYSTEMS > COASTAL HABITAT > CPD ABUNDANCE IN MARINE PHYTOPLANKTON
BIOSPHERE > AQUATIC ECOSYSTEMS > COASTAL HABITAT > MARINE PHYTOPLANKTON SPECIES COMPOSITION
BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT
BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > POPULATION DYNAMICS
Quality Immunochemical thymine dimer detection, using monoclonal H3
antibody. Detection limit < 1 CPD.MB.
Attenuation coefficients for Biologically Effective UVBR, by DNA
dosimeter incubations: 24h incubations at various water depths.
Salinity: ProFi Lab salinity Probe Accuracy 0.1 psu.
Phytoplankton species composition: classical microscopy.
Access Constraints None
Use Constraints Please cite the data properly.
Data Set Progress
Role: TECHNICAL CONTACT
Role: DIF AUTHOR
Phone: +31 (0)50 363 2286
Fax: +31 (0)50 363 2261
Email: A.M.T.Piquet at rug.nl
Ocean Ecosystems, ESRIG, University of Groningen Kerklaan 30
City: Haren GN
Province or State: Groningen
Postal Code: 9750 AA
Country: The Netherlands
A.G.J. Buma, M. K. de Boer, P. Boelen. 2000. Depth distributions of
DNA damage in Antarctic marine phyto- and bacterioplankton exposed to
summertime ultraviolet radiation. J. Phycol., in press.
Creation and Review Dates
DIF Creation Date: 2000-06-22
Last DIF Revision Date: 2004-08-11