Distribution of Novel Infectious Agent in Healthy and Diseased Bicolor Damselfish in Florida and the Caribbean
Entry ID: RSMAS_MSC001b

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Summary
Abstract: The disease damselfish neurofibromatosis (DNF) affects bicolor damselfish
(Stegastes partitus) on reefs in Florida. This disease consists of peripheral
nerve sheath and pigment cell tumors that are eventually fatal. The
development of DNF is correlated with the appearance of unusual,
extrachromosomal DNAs in affected fish that appear to be the genome of an
undescribed, virus-like agent. Probes derived from these sequences were used to
determine the distribution of this agent in diseased and health fish from
several reefs in South Florida and from selected locations elsewhere in the
range of this species. These analyses demonstrated that naturally diseased fish
exhibited high levels of these DNAs in tumors and, to a lesser extent, in
unaffected tissues. Fish with experimentally induced DNF exhibited similar
levels of this DNA. Healthy adult fish had either no detectable levels of this
DNA or very low levels, depending on the sensitivity of the detection
technique used. Healthy adults from high disease prevalence reefs were more
likely to test positive for this DNA than those from low disease reefs.
Juvenile damselfish never contained detectable levels of this agent. Very low
levels of this DNA were also detected in healthy fish from the Caribbean and
the Bahamas. Dose-response experiments using tumor-derived cell lines indicated
that tumor development was directly related to exposure dosages and that very
low concentrations of this material did not yield tumor development. Taken
together, these data indicate that high levels of this agent were correlated
with the appearance of this disease and the very low levels often observed in
healthy fish were not a predictor of tumor development. To determine
correlations between exposure dosages or low concentrations of DNA and the
development of tumors in healthy fish.


Spatial Data Organization Information - Indirect Spatial Reference:
Named reefs and reef systems: Fowey Rocks, North Dry Rocks, Little Grecian
Reef, Ajax Reef, Dry Tortugas
Named Regions: Bahamas (Bimini), Belize, Curacao,
Haiti, Jamaica, the Turks and Caicos

Direct Spatial Reference: Vector Spatial
Reference Information - Horizontal Coordinate
System Definition - Geodetic Model:

Geographic Coverage
 N: 23.0 S: 19.0  E: -69.0  W: -74.0

Data Set Citation
Dataset Originator/Creator: Campbell, C.E., Schmale, M.C.
Dataset Title: Distribution of novel infectious agent in healthy and diseased bicolor damselfish in Florida and the
Caribbean

Dataset Series Name: Marine Biology
Dataset Release Date: 2001
Dataset Release Place: Dordrecht, The Netherlands
Dataset Publisher: Springer-Verlag GmbH


Location Keywords
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > CARIBBEAN SEA
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > CARIBBEAN SEA > CURACAO
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > CARIBBEAN SEA > TURKS AND CAICOS ISLANDS
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > CARIBBEAN SEA > BAHAMAS
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > CARIBBEAN SEA > HAITI
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > CARIBBEAN SEA > JAMAICA
CONTINENT > NORTH AMERICA > UNITED STATES OF AMERICA > FLORIDA


Science Keywords
BIOLOGICAL CLASSIFICATION >ANIMALS/VERTEBRATES >FISH >RAY-FINNED FISHES >ACTINOPTERYGII
BIOLOGICAL CLASSIFICATION >ANIMALS/VERTEBRATES >FISH    [Definition]
BIOLOGICAL CLASSIFICATION >VIRUSES
BIOSPHERE >AQUATIC ECOSYSTEMS >COASTAL HABITAT    [Definition]
BIOSPHERE >AQUATIC ECOSYSTEMS >REEF HABITAT    [Definition]


ISO Topic Category
BIOTA
OCEANS


Access Constraints
None


Use Constraints
Contact Dr. Michael Schmale or the Principle Investigator for data use
constraints. No liability assumed.


Keywords
Damselfish neurofibromatosis (DNF)
Bicolor damselfish (Stegastes partituts)
Reefs
Virus-like agent
Disease distribution
Fowey Rocks
North Dry Rocks
Little Grecian Reef
Ajax Reef
Dry Tortugas
Bahamas (Bimini)
Belize
Curacao
Haiti
Jamaica
Turks and Caicos
Florida Keys


Data Set Progress
COMPLETE


Originating Center
RSMAS


Data Center
Rosenstiel School of Marine and Atmospheric Science, University of Miami    [Information]
Data Center URL: http://www.rsmas.miami.edu/

Data Center Personnel
Name: MICHAEL S. SCHMALE
Phone: 305-421-4140
Fax: 305-421-4600
Email: mschmale at rsmas.miami.edu
Contact Address:
MBF/RSMAS
University of Miami
4600 Rickenbacker Cswy
City: Miami
Province or State: FL
Postal Code: 33149-1098
Country: USA



Distribution
Fees: none


Personnel
MICHAEL S. SCHMALE
Role: INVESTIGATOR
Phone: 305-421-4140
Fax: 305-421-4600
Email: mschmale at rsmas.miami.edu
Contact Address:
MBF/RSMAS
University of Miami
4600 Rickenbacker Cswy
City: Miami
Province or State: FL
Postal Code: 33149-1098
Country: USA


Publications/References
Schmale, M.C., P.D.L. Gibbs and C.E. Campbell (2002) A virus-like agent
associated with neurofibromatosis in damselfish. Diseases Aquatic Org. 49:107-115.

Campbell, C.E. and M.C. Schmale (2001) Distribution of a novel infectious agent in healthy and tumored bicolor damselfish in Florida and the Caribbean. Marine Biology 139:777-786.

Campbell, C.E., P.D.L. Gibbs and M.C. Schmale (2001) Progression of infection and tumor development in damselfish. Marine Biotechnology 3:S107-S114

Gibbs, P.D.L. & M.C. Schmale (2000) GFP as a genetic marker scorable throughout the lifecycle of transgenic zebrafish. Marine Biotechnology 2:107-125.

Corrales, J., L.B. Nye, S.D. Baribeau, G. Gassman & M.C. Schmale. (2000) Characterization of scale abnormalities in pinfish, Lagodon rhomboides, from Biscayne Bay, Florida. Environ. Biol. Fishes 57:205-220.

Creation and Review Dates
DIF Creation Date: 2005-07-15
Last DIF Revision Date: 2007-03-06

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