Related URL

Link: GET DATA Description: Download point for the data Link: VIEW PROJECT HOME PAGE Description: Public information for AAS project 3127 Link: VIEW RELATED INFORMATION Description: Citation reference for this metadata record and dataset

Geographic Coverage

N: -54.0

S: -67.0

E: 160.0

W: 60.0

Temporal Coverage

Start Date: 2009-09-30

Stop Date: 2012-03-31

Location Keywords

OCEAN > SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR

Science Keywords

OCEANS >OCEAN CHEMISTRY >NUTRIENTS    [Definition]

BIOLOGICAL CLASSIFICATION >BACTERIA/ARCHAEA    [Definition]

ISO Topic Category

BIOTA OCEANS

Platform

SHIPS    [Information]

LABORATORY    [Information]

Quality

Values provided in temporal and spatial coverage are approximate only. Taken from the 2009-2010 Progress Report: Variations to work plan or objectives: No field work to collect samples was able to be performed, all activities to date have been laboratory based. 1. PCR analysis of PR in 65 Antarctic bacterial strains (obtained from previous AAD-supported activity) by marine science student Jaume Bibloni. Results to date indicate extensive presence of PR genes in Antarctic lake bacteria including undescribed species. PR presence in sea-ice bacteria was more restricted. Obvious strain-dependency occurs in PR distribution. Sequence data obtained for most PR positive strains. Limitations in the analysis include the need to use highly degenerate primer oligonucleotides (due to high level of variability PR sequences at the nucleotide level). Work is ongoing to retest several strains giving equivocal results in the PCR asays. 2. Proteomics analysis has been initiated (Clare Rutherford Honours student) for analysis of response of Psychroflexus torquis to light and dark conditions grown at 2 C (grown in a modified marine medium). The proteomics involves shotgun analysis using a 2-dimensional HPLC separation of trypsinised protein extracts (recovered using the QProteome proteoin extraction kit and extraction with membrane protein surfactant C7BzO, Sigma-Aldrich) coupled to nano-flow LTQ-Orbitrap mass spectrometry. This work is done in collaboration with Dr Edwin Lowe, Central Sciences Laboratory. The goal of this experiment is to determine whether the presence of light induces PR translation (abundance) and it cognate carotenoid hydroxylase as well as other changes to the proteome. Based on recent experiments on other bacteria (E. coli and L. monocytogenes) as much as 50% of the proteome can be recovered using this approach (termed "MudPit" - multidimensional protein information technology). This will be first time such ana analysis has been performed on a marine bacterial species. 3. Growth experiments have been initiated by Clare Rutherford and Jaume Bibloni to determine conditions inducing growth rates and yields in the presence of light for the PR-bearing species P. torquis. The conditions being focussed on are nutritional. The experiments involve utilisation of 96-well trays and spectrophotometry using a plate reader. Initial results suggest that light induces growth when certain critical nutrients are low, potentially either iron and/or certain vitamins (cobalamin), suggesting that PR may aid in generating a proton gradient helping drive enhanced nutrient uptake.

Access Constraints

These data are not yet publicly available.

Use Constraints

This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_3127 when using these data.

Keywords

Bacteria

proteorhodopsin

PCR

Data Set Progress

IN WORK

Data Center

Australian Antarctic Data Centre, Australia    [Information] Data Center URL: http://data.aad.gov.au Data Center Personnel Name: DATA OFFICER AADC Phone: +61 3 6232 3244 Fax: +61 3 6232 3351 Email: metadata at aad.gov.au Contact Address: Australian Antarctic Division 203 Channel Highway City: Kingston Province or State: Tasmania Postal Code: 7050 Country: Australia

Distribution

Distribution_Media: HTTP Distribution_Size: 2.8 MB Distribution_Format: Excel, PDF Fees: Free

Personnel

JOHN BOWMAN Role: INVESTIGATOR Role: TECHNICAL CONTACT Phone: +61 3 6226 6380 Fax: +61 3 6226 2642 Email: john.bowman at utas.edu.au Contact Address: School of Agricultural Science University of Tasmania Private Bag 54 City: Hobart Province or State: Tasmania Postal Code: 7001 Country: Australia

DAVE CONNELL Role: DIF AUTHOR Phone: +61 3 6232 3244 Fax: +61 3 6232 3351 Email: dave.connell at aad.gov.au Contact Address: Australian Antarctic Division 203 Channel Highway City: Kingston Province or State: Tasmania Postal Code: 7050 Country: Australia