Molecular analysis of microbiota trapped in ancient antarctic glacial ice
Entry ID: ASAC_2763

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Summary
Abstract: Metadata record for data from ASAC Project 2763
See the link below for public details on this project.

Ancient Antarctic glacial ice is a potential resource of trapped microorganisms dating back several hundreds of thousand years that give a snapshot of the past. Nucleic acid, such as DNA, has been identified in samples as old as these from Bacteria, Archaea and Viruses, and this will be the focus of this study. Outcomes of this research will determine the type of organisms that become trapped in these ancient samples, and whether they are able to survive such an extreme condition, and may even lead to novel species being discovered, or even new genes and products.

Project objectives:
Determine the type of microorganisms that become trapped in ancient Antarctic glacial ice and ascertain whether glacial ice in Antarctica harbours biota that are of evolutionary or biotechnological interest.

Public summary of the season progress:
Ancient glacial ice samples were collected from ice cliff areas located near Casey Station during the just recent Summer expedition. Ice samples were transported back to Australia and will be subject to 454 sequencing analysis in the next few months in collaboration with Prof. Alan Cooper, Centre for Ancient DNA, University of Adelaide. Other work being performed was completed included an initial molecular-based microbial survey of unusual alkaline, permanently ice-covered, continental lakes located in the Framnes Mountains, inland from Mawson

The first download file contains:
10 files
File 1. Chemical and oxygen isotope data for Law Dome ice cores that were used in microbiological studies (isolation and DNA analyses).
File 2. Chemical and oxygen isotope data for an Amery Ice Shelf ice core that was used in microbiological studies (isolation and DNA analyses).
File 3. 16S rRNA gene sequence data obtained from Law Dome ice core samples. All sequences are clones derived after direct PCR amplification of DNA extracts, no isolates were obtained.
File 4. 16S rRNA gene sequence data obtained from Amery Ice Shelf ice core sample. All sequences are clones derived after direct PCR amplification of DNA extracts, no isolates were definitively obtained.
File 5. Soil and similar samples obtained from either the Vestfold Hills, Eastern Antarctica or from Macquarie Harbour. These soils are the source of actinobacteria screened in the project for antimicrobial activity.
File 6. Actinobacteria/actinomycete isolates information detailing isolation procedure, colonial/cellular characteristics, and tentative identification. Barcode system was used to track isolates. Represents the "(University of Tasmania Antarctic Actinobacteria) UTAA" collection.
File 7. Preliminary antimicrobial screening trial data for 1267 Antarctic actinobacterial isolates against 5 strains of Listeria monocytogenes. A secondary screen was performed to identify those with reliable bioactivity.
File 8. Broader analysis of antimicrobial activities of Antarctic isolates against a panel of bacterial pathogenic bacteria. All strains were identified to species level within the genus Streptomyces.
File 9. Hydrocarbon profiles of selected Antarctic actinobacterial strains against a range of aliphatic and polycyclic hydrocarbons.
File 10. Phenotypic, chemotaxonomic and identification (by 16S rRNA gene sequencing) data of Antarctic hydrocarbon degrading strains.

The second download file contains:
3 files:
File 1. Framnes Mountain epiglacial lake sample data. Indicates lakes sampled, location, chemical data (pH, temperature).
File 2. Cloned 16S rRNA gene sequences obtained from Patterned Lake water DNA extracts. All sequnces are in FASTA format.
File 3. Cloned 16S rRNA gene sequences obtained from Sonic Lake water DNA extracts. All sequnces are in FASTA format.

Related URL
Link: GET DATA
Description: Download point for the data - part 1


Link: GET DATA
Description: Download point for the data - part 2


Link: VIEW PROJECT HOME PAGE
Description: Public information for ASAC project 2763


Link: VIEW RELATED INFORMATION
Description: Citation reference for this metadata record and dataset


Geographic Coverage
 N: -66.0 S: -67.0  E: 111.0  W: 110.0

Temporal Coverage
Start Date: 2006-10-01
Stop Date: 2007-03-31


Location Keywords
CONTINENT > ANTARCTICA > Casey
GEOGRAPHIC REGION > POLAR


Science Keywords
CRYOSPHERE >GLACIERS/ICE SHEETS >GLACIERS    [Definition]
BIOLOGICAL CLASSIFICATION >BACTERIA/ARCHAEA    [Definition]


ISO Topic Category
BIOTA
CLIMATOLOGY/METEOROLOGY/ATMOSPHERE
ENVIRONMENT


Platform
FIELD SURVEYS    [Information]


Quality
The Dates provided in temporal coverage are approximate only, and represent the beginning and end of the 2006 - 2007 Antarctic seasons.

The latitudes and longitudes provided in spatial coverage are approximate only.


Access Constraints
The data are publicly available for download from the provided URLs.


Use Constraints
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2763 when using these data.


Keywords
glaciers
bacteria
viruses
DNA


Data Set Progress
COMPLETE


Data Center
Australian Antarctic Data Centre, Australia    [Information]
Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia



Distribution
Distribution_Media: HTTP
Distribution_Size: 56 kb
Distribution_Format: csv, text
Fees: Free

Distribution_Media: HTTP
Distribution_Size: 7 kb
Distribution_Format: csv, text
Fees: Free


Personnel
JOHN BOWMAN
Role: INVESTIGATOR
Role: TECHNICAL CONTACT
Phone: +61 3 6226 6380
Fax: +61 3 6226 2642
Email: john.bowman at utas.edu.au
Contact Address:
School of Agricultural Science
University of Tasmania
Private Bag 54
City: Hobart
Province or State: Tasmania
Postal Code: 7001
Country: Australia


DAVE J. CONNELL
Role: DIF AUTHOR
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: dave.connell at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia



Creation and Review Dates
DIF Creation Date: 2006-07-27
Last DIF Revision Date: 2014-08-29

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