Global Change Master Directory (GCMD)

Data Sets

Services / Tools

Ancillary Descriptions

Are you using a browser that doesn't support JavaScript or have you disabled JavaScript?

If you have disabled JavaScript, you must re-enable JavaScript to use this page.
Or you can view this record using the Text Only Format.

Diversity and metabolic abilities of antarctic bacteria - Polyhydroxyalkanoate production by Antarctic soil bacteria

Entry ID: ASAC_2341_PHA

[ Get Data ]
[ Update this Record ]

Summary

Abstract: Bacterial isolates were obtained from soil samples collected from various locations around Casey station. Soil samples were serial diluted in sterile water and plated on low-strength nutrient agar containing cycloheximide. The agar plates were incubated at 5 degrees C until colonies were observed. Bacterial colonies were picked and purified on fresh agar plates.

The bacterial isolates were ... screened for the production of polyhydroxyalkanoates (PHA) by growing them in nitrogen-limiting medium. PHA in the cells was detected by Nile Blue A staining and by Nile Red staining of the colonies. PHA-producing isolates were tested with individual carbon sources i.e. glucose, sodium octanoate and saponified palm kernel oil. The intracellular PHA was extracted by chloroform, precipitated by methanol, and analysed by gas-chromatography (GC) to determine the monomer compositions. Selected PHA-producing isolates were identified by their 16S rRNA gene sequences.

Two main groups of PHA-producing bacteria were identified from among the isolates: Janthinobacterium spp. which produce polyhydroxybutyrate (PHB) from glucose, and Pseudomonas spp. which produce medium-chain-length PHA (mcl-PHA) from octanoate. Some but not all of the Pseudomonas isolates were able to produce mcl-PHA from glucose. The monomer composition of the mcl-PHA produced by these Pseudomonas strains was found to be related to the carbon substrates fed to the bacteria.

In selected isolates, the PHA biosynthesis genes (phaC) and associated PHA depolymerase gene (phaZ) will be analysed and compared with those from non-antarctic bacteria.

The attached excel file lists the PHA-producing bacterial isolates obtained from various soil samples, and their closest homology to GenBank entry and type culture based on the 16S rRNA gene sequence.

Feb 2004 (soil samples collected by member from another research group) and
Dec 2005 (soil samples collected by C.W.Chong)

Soil samples were collected from the following locations:
- Around Casey station - (Red Shed, Lab building, etc)
- Peterson Island
- Around Wilkes Land (Wilkes Land, Whitney Point, Thala Valley)
- Browning Peninsula
- Mitchell Peninsula

Irene K. P. TAN - Project leader
Yuh Shan GOH - isolated and screened bacteria for polyhydroxyalkanoates (PHA); identified the PHA-producing bacterial isolates; characterised the PHA
Chun Wie CHONG - collected the soil samples from around Casey station in December 2005

Related URL

Link: GET DATA

Description: Download point for the accession numbers

Link: GET SERVICE

Description: GenBank Website

Link: VIEW PROJECT HOME PAGE

Description: Public information for AAS project 2341

Link: VIEW RELATED INFORMATION

Description: Citation reference for this metadata record and dataset

Geographic Coverage

(Click for Interactive Map)

Spatial coordinates

N: -66.28

S: -66.28

E: 110.54

W: 110.54

N: -66.45

S: -66.45

E: 110.5

W: 110.5

N: -66.27

S: -66.27

E: 110.54

W: 110.54

N: -66.34

S: -66.34

E: 110.55

W: 110.55

N: -66.31

S: -66.31

E: 110.54

W: 110.54

Data Set Citation

Dataset Originator/Creator: Tony Gow, Deb Meese

Dataset Title: Physical and Structural Properties of the Siple Dome Ice Cores

Dataset Series Name: WAISCORES

Dataset Release Place: Boulder, CO U.S.A.

Dataset Publisher: National Snow and Ice Data Center (NSIDC)

Data Presentation Form: images

Online Resource: http://nsidc.org/data/nsidc-0128.html

Temporal Coverage

Start Date: 1996-01-01

Stop Date: 2000-12-31

Location Keywords

CONTINENT > ANTARCTICA

GEOGRAPHIC REGION > POLAR

Science Keywords

CRYOSPHERE > SNOW/ICE > ICE DEPTH/THICKNESS

CRYOSPHERE > SNOW/ICE > ICE GROWTH/MELT

ISO Topic Category

CLIMATOLOGY/METEOROLOGY/ATMOSPHERE

GEOSCIENTIFIC INFORMATION

INLAND WATERS

Platform

GROUND STATIONS

GROUND-BASED OBSERVATIONS

Instrument

CORING DEVICES

Ancillary Keywords

AGDC

Antarctica

Data Set Progress

COMPLETE

Data Center

National Snow and Ice Data Center

Data Center URL: http://nsidc.org
Dataset ID: NSIDC-0128

Data Center Personnel
Name: NSIDC USER SERVICES Phone: +1 (303) 492-6199 Fax: +1 (303) 492-2468 Email: nsidc at nsidc.org Contact Address: National Snow and Ice Data Center CIRES, 449 UCB University of Colorado City: Boulder Province or State: CO Postal Code: 80309-0449 Country: USA

NSIDC Antarctic Glaciological Data Center

Data Center URL: http://nsidc.org/agdc/
Dataset ID: NSIDC-0128

Data Center Personnel
Name: NSIDC AGDC USER SERVICES Phone: +1 (303) 492-6199 Fax: +1 (303) 492-2468 Email: nsidc at nsidc.org Contact Address: National Snow and Ice Data Center CIRES, 449 UCB University of Colorado City: Boulder Province or State: CO Postal Code: 80309-0449 Country: USA

Distribution

Distribution Media: ftp

Distribution Size: 2.2 MB .jpg files, 493 KB .gif files, 9 KB text files

Distribution Format: .jpg and .gif image files and text files

Personnel

TONY GOW

Role: INVESTIGATOR

Contact Address:

Cold Regions Research and Engineering Laboratory

72 Lyme Road

City: Hanover

Province or State: NH

Postal Code: 03755

Country: USA

DEB MEESE

Role: INVESTIGATOR

Phone: (603) 646-4594

Email: dmeese at hanover-crrel.army.mil

Contact Address:

Cold Regions Research and Engineering Laboratory

72 Lyme Road

City: Hanover

Province or State: NH

Postal Code: 03755

Country: USA

NSIDC USER SERVICES

Role: TECHNICAL CONTACT

Phone: +1 (303) 492-6199

Fax: +1 (303) 492-2468

Email: nsidc at nsidc.org

Contact Address:

National Snow and Ice Data Center

CIRES, 449 UCB

University of Colorado

City: Boulder

Province or State: CO

Postal Code: 80309-0449

Country: USA

Creation and Review Dates

DIF Creation Date: 2003-03-07

Last DIF Revision Date: 2011-06-30

Reformat as FGDC document

View Text Only Format

[ Update this Record ]