Science Keywords>LAND SURFACE
|
Diversity and metabolic abilities of Antarctic bacteria - DGGE fingerprinting of bacteria Entry ID: ASAC_2341_DGGE |
||||||||
|
[
Update this Record
]
|
Summary
|
Abstract:
Bacterial community structures in soils collected from eight sites around Casey Station, Antarctica, were investigated using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rRNA gene fragments. Higher bacterial diversity was found in soils from protected or relatively low human-impacted sites in comparison to highly impacted sites. However, the highest diversity was detected in samples from Wilkes Tip, a former waste disposal site that has been undisturbed for the last 50 years. Comparison of community structure based on non-metric multidimensional scaling plots revealed that all sites, except the hydrocarbon-contaminated (oil spill) site, were clustered with a 45% similarity. A total of 23 partial 16S rRNA gene sequences were obtained from the excised DGGE bands, with the majority of the sequences closely related to those of the Cytophaga-Flexibacter-Bacteroides group. No significant correlation was established between environmental variables, including soil pH, electrical conductivity, carbon, nitrogen, water content and heavy metals, with bacterial diversity across the eight study sites. Irene K. P. Tan - Project leader C. W. Chong - sample collection, molecular and statistical analyses G. Y. Annie Tan - provided some advice in molecular analysis Richard C. S. Wong - provided advice in chemical analyses Martin J. Riddle - assisted in manuscript writing and provided advice in metal analyses Locations used in this experiment were: - Casey Red Shed - Oil Spill site - Thala Valley - Wilkes Tip - Browning Peninsula - Mitchell Peninsula - ASPA 135 - ASPA 136 |
Related URL
|
Link:
VIEW PROJECT HOME PAGE
Description: Public information for AAS project 2341 Link: VIEW RELATED INFORMATION Description: Citation reference for this metadata record and dataset |
Geographic Coverage
| N: -66.2827 | S: -66.2827 | E: 110.5225 | W: 110.5225 |
|
| N: -66.2816 | S: -66.2816 | E: 110.5252 | W: 110.5252 |
|
| N: -66.2802 | S: -66.2802 | E: 110.5372 | W: 110.5372 |
|
| N: -66.2597 | S: -66.2597 | E: 110.5394 | W: 110.5394 |
|
| N: -66.4722 | S: -66.4722 | E: 110.5497 | W: 110.5497 |
|
| N: -66.3141 | S: -66.3141 | E: 110.5486 | W: 110.5486 |
|
| N: -66.2819 | S: -66.2819 | E: 110.5388 | W: 110.5388 |
|
| N: -66.2522 | S: -66.2522 | E: 110.5375 | W: 110.5375 |
|
Temporal Coverage
| Start Date: 2005-12-11 |
| Stop Date: 2005-12-18 |
Location Keywords
|
CONTINENT
> ANTARCTICA
> Windmill Islands
GEOGRAPHIC REGION > POLAR |
Science Keywords
| BIOLOGICAL CLASSIFICATION >BACTERIA/ARCHAEA [Definition] |
| LAND SURFACE >SOILS >MICROFAUNA [Definition] |
ISO Topic Category
|
BIOTA
|
Platform
| FIELD INVESTIGATION [Information] |
| FIELD SURVEYS [Information] |
| LABORATORY [Information] |
Quality
| The DGGE studies were to compare bacterial diversity between sites, based on band patterns. The 16S DNA fragments used were very short, about 500 bp only. The sequence of individual bands were not sufficient to identify the bacteria. Hence, there are no accession numbers. |
Access Constraints
| There are no data from this part of the project - see the quality field for further information. |
Use Constraints
|
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_234... when using these data. |
Keywords
| Bacteria |
| denaturing gradient gel electrophoresis |
| DGGE |
Data Set Progress
| COMPLETE |
Originating Center
| Australian Antarctic Division |
Data Center
|
Australian Antarctic Data Centre, Australia
[Information]
Data Center URL: http://data.aad.gov.au
| |||
Personnel
|
IRENE KIT-PING
TAN Role: INVESTIGATOR Role: TECHNICAL CONTACT Phone: +60 3 7967 6792 Fax: +60 3 7967 4178 Email: itan at um.edu.my Contact Address: University of Malaya FACULTY OF SCIENCE Institute of Biological Sciences City: Kuala Lumpur Postal Code: 50603 Country: Malaysia |
|
CHUN WIE
CHONG Role: TECHNICAL CONTACT Email: cchhoonngg81 at yahoo.com Contact Address: International Medical University No. 126, Jalan Jalil Perkasa 19, Bukit Jalil City: Kuala Lumpur Postal Code: 57000 Country: Malaysia |
|
ANNIE
GEOK YUAN
TAN Role: TECHNICAL CONTACT Phone: 60379674634 Fax: 60379605935 Email: gytan at um.edu.my Contact Address: Institute of Biological Sciences University of Malaya City: Kuala Lumpur Postal Code: 50603 Country: Malaysia |
|
RICHARD
CS
WONG Role: TECHNICAL CONTACT Email: Richard at um.edu.my Contact Address: University of Malaya FACULTY OF SCIENCE Department of Chemistry City: Kuala Lumpur Postal Code: 50603 Country: Malaysia |
|
MARTIN
RIDDLE Role: TECHNICAL CONTACT Phone: +61 3 6232 3573 Fax: +61 3 6232 3351 Email: martin.riddle at aad.gov.au Contact Address: Australian Antarctic Division 203 Channel Highway City: Kingston Province or State: Tasmania Postal Code: 7050 Country: Australia |
|
DAVE
CONNELL Role: DIF AUTHOR Phone: +61 3 6232 3244 Fax: +61 3 6232 3351 Email: dave.connell at aad.gov.au Contact Address: Australian Antarctic Division 203 Channel Highway City: Kingston Province or State: Tasmania Postal Code: 7050 Country: Australia |
Publications/References
Chong C. W., Tan G. Y., Wong R. C. S. Riddle M. J., Tan I. K. P (2009), DGGE fingerprinting of bacteria in soils from eight ecologically different sites around Casey Station, Antarctica, Polar Biology, 32, 853-860, doi:DOI:10.1007/s00300-009-0585-6
Creation and Review Dates
DIF Creation Date:
2012-11-12
Last DIF Revision Date:
2012-12-20
| [ Update this Record ] |



