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Diversity and metabolic abilities of antarctic bacteria - Polyhydroxyalkanoate production by Antarctic soil bacteria

Entry ID: ASAC_2341_PHA

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Summary

Abstract: Bacterial isolates were obtained from soil samples collected from various locations around Casey station. Soil samples were serial diluted in sterile water and plated on low-strength nutrient agar containing cycloheximide. The agar plates were incubated at 5 degrees C until colonies were observed. Bacterial colonies were picked and purified on fresh agar plates.

The bacterial isolates were ... screened for the production of polyhydroxyalkanoates (PHA) by growing them in nitrogen-limiting medium. PHA in the cells was detected by Nile Blue A staining and by Nile Red staining of the colonies. PHA-producing isolates were tested with individual carbon sources i.e. glucose, sodium octanoate and saponified palm kernel oil. The intracellular PHA was extracted by chloroform, precipitated by methanol, and analysed by gas-chromatography (GC) to determine the monomer compositions. Selected PHA-producing isolates were identified by their 16S rRNA gene sequences.

Two main groups of PHA-producing bacteria were identified from among the isolates: Janthinobacterium spp. which produce polyhydroxybutyrate (PHB) from glucose, and Pseudomonas spp. which produce medium-chain-length PHA (mcl-PHA) from octanoate. Some but not all of the Pseudomonas isolates were able to produce mcl-PHA from glucose. The monomer composition of the mcl-PHA produced by these Pseudomonas strains was found to be related to the carbon substrates fed to the bacteria.

In selected isolates, the PHA biosynthesis genes (phaC) and associated PHA depolymerase gene (phaZ) will be analysed and compared with those from non-antarctic bacteria.

The attached excel file lists the PHA-producing bacterial isolates obtained from various soil samples, and their closest homology to GenBank entry and type culture based on the 16S rRNA gene sequence.

Feb 2004 (soil samples collected by member from another research group) and
Dec 2005 (soil samples collected by C.W.Chong)

Soil samples were collected from the following locations:
- Around Casey station - (Red Shed, Lab building, etc)
- Peterson Island
- Around Wilkes Land (Wilkes Land, Whitney Point, Thala Valley)
- Browning Peninsula
- Mitchell Peninsula

Irene K. P. TAN - Project leader
Yuh Shan GOH - isolated and screened bacteria for polyhydroxyalkanoates (PHA); identified the PHA-producing bacterial isolates; characterised the PHA
Chun Wie CHONG - collected the soil samples from around Casey station in December 2005

Related URL

Link: GET DATA

Description: Download point for the accession numbers

Link: GET SERVICE

Description: GenBank Website

Link: VIEW PROJECT HOME PAGE

Description: Public information for AAS project 2341

Link: VIEW RELATED INFORMATION

Description: Citation reference for this metadata record and dataset

Geographic Coverage

(Click for Interactive Map)

Spatial coordinates

N: -66.28

S: -66.28

E: 110.54

W: 110.54

N: -66.45

S: -66.45

E: 110.5

W: 110.5

N: -66.27

S: -66.27

E: 110.54

W: 110.54

N: -66.34

S: -66.34

E: 110.55

W: 110.55

N: -66.31

S: -66.31

E: 110.54

W: 110.54

Data Set Citation

Dataset Release Date: 2012-11-13

Temporal Coverage

Start Date: 2004-02-01

Stop Date: 2004-02-29

Start Date: 2005-12-01

Stop Date: 2005-12-31

Location Keywords

CONTINENT > ANTARCTICA > Windmill Islands

GEOGRAPHIC REGION > POLAR

Science Keywords

BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA

LAND SURFACE > SOILS > MICROFAUNA

ISO Topic Category

BIOTA

Platform

FIELD INVESTIGATION

FIELD SURVEYS

LABORATORY

Access Constraints The accession numbers for accessing these data at GenBank are available at the provided URL.

Use Constraints This data set conforms to the PICCCBY Attribution License
(http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_234... when using these data.

Ancillary Keywords

Bacteria

polyhydroxyalkanoates

PHA

Data Set Progress

COMPLETE

Data Center

Australian Antarctic Data Centre, Australia

Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC Phone: +61 3 6232 3244 Fax: +61 3 6232 3351 Email: metadata at aad.gov.au Contact Address: Australian Antarctic Division 203 Channel Highway City: Kingston Province or State: Tasmania Postal Code: 7050 Country: Australia

Distribution

Distribution Media: HTTP

Distribution Size: 7 kb

Distribution Format: Excel

Fees: Free

Personnel

IRENE KIT-PING TAN

Role: INVESTIGATOR

Role: TECHNICAL CONTACT

Phone: +60 3 7967 6792

Fax: +60 3 7967 4178

Email: itan at um.edu.my

Contact Address:

University of Malaya

FACULTY OF SCIENCE

Institute of Biological Sciences

City: Kuala Lumpur

Postal Code: 50603

Country: Malaysia

Publications/References

Goh Y.S. and Tan I.K.P. (2012), Polyhydroxyalkanoate production by Antarctic soil bacteria isolated from Casey Station and Signy Island, Microbiological Research, 167, 211-219, doi:doi: 10.1016/j.micres.2011.08.002

Creation and Review Dates

DIF Creation Date: 2012-11-12

Last DIF Revision Date: 2012-11-13

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