Climate change and carbon gain in Antarctic mosses - Drying Responses
Entry ID: AAS_2780_dryingresponse

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Summary
Abstract: We compared the drying responses of clumps of moss from Casey Station, Antarctica, evaluating the changes in mass, water content, photosynthesis (measured by chlorophyll fluorescence), cellular solute concentration (measured by differential scanning calorimetry) and cellular dimensions.

Drying was performed at different temperatures and VPDs to evaluate the relative impacts of temperature and humidity on moss physiology.

This dataset is comprised of several spreadsheets (all in comma separated values format). The primary data sheet (Moss-desiccation tolerance Master Datasheet.csv) compiles the overall experimental data, including means derived from several other data sheets.

Moss-fluorescence Datasheet.csv reports data from Walz MaxiPAM chlorophyll fluorescence measurements of moss clumps at each timepoint.

Moss-fluorescence micro Datasheet.csv reports data from Walz Microscope PAM chlorophyll fluorescence measurements of individual leaves and shoots sampled at each timepoint and kept under oil to retain hydration.

Moss-meas Datasheet.csv reports the dimensions (area, perimeter, length and width) of cells from individual leaves sampled at each timepoint and kept under oil to retain hydration.
Solute concentration of the shoots was determined by performing differential scanning calorimetry (DSC) on shoots sampled at each timepoint.

To convert the melting/freezing points measured to concentrations requires calibration, the data for which are provided in Moss-DSC calib.csv.

The data are laid out with columns for each variable considered, and rows for each sample. The columns for each spreadsheet are as follows:

Moss-desiccation tolerance Master Datasheet.csv:
Date-date of experiment
Run-experimental run (1 to 18)
ClumpSp-dominant species of moss clump: C-Ceratodon purpureus; S- Schistidium antarctici; BC- Bryum pseudotriquetrum and Ceratodon purpureus codominant Species-species of shoot examined in calorimetry/microscopy: B- Bryum pseudotriquetrum; C-Ceratodon purpureus; S- Schistidium antarctici Replicate-replicate (here equal to Run) Pseudoreplicates-replicates of the same species during the same run Temp-Treatment temperature: 8 degrees C, 16 degrees C or 24 degrees C VPD-Vapour pressure differential of treatment: 0.2kPa, 0.5kPa or 1.2kPa Timepoint-Sampling timepoint (0 = initial conditions) Time-Time in hours since start of experimental run Treatment-Experimental phase: Drying, Recovery (rehydration following drying process), ReRun (second round of drying following recovery) Basket mass-Mass in grams of basket containing moss clump Basket + moss mass-Mass in grams of basket and moss sample Fresh mass-Fresh mass in grams of moss clump (calculated) Dry mass-Mass in grams of oven-dried moss clump RWC-Relative water content of moss clump in g water per g dry mass Percentage of initial RWC-Relative water content as a percentage of initial RWC Mean Fo-Mean Fo of clump (from Moss-fluorescence Datasheet.csv) Sd Fo-Standard deviation of the Fo of the clump (from Moss-fluorescence Datasheet.csv) Mean Fm-Mean Fo of clump (from Moss-fluorescence Datasheet.csv) Sd Fm-Standard deviation of the Fo of the clump (from Moss-fluorescence Datasheet.csv) Mean Fv.Fm-Mean Fo of clump (from Moss-fluorescence Datasheet.csv) Sd Fv.Fm-Standard deviation of the Fo of the clump (from Moss-fluorescence Datasheet.csv) Mean Fo'-Mean Fo of clump (from Moss-fluorescence Datasheet.csv) Sd Fo'-Standard deviation of the Fo of the clump (from Moss-fluorescence Datasheet.csv) Mean Fm'-Mean Fo of clump (from Moss-fluorescence Datasheet.csv) Sd Fm'-Standard deviation of the Fo of the clump (from Moss-fluorescence Datasheet.csv) Tin mass-Mass in grams of the empty calorimetry tin Tin + moss wet mass-Mass in grams of moss shoot and calorimetry tin Tin + moss dry mass-Mass in grams of oven-dried moss shoot and calorimetry tin RWC stems DSC-Relative water content of shoot used for calorimetry in g water per g dry mass Tm-1-Melting point in uncorrected degrees C of shoot water (first replicate) PeakT1-Melting peak in uncorrected degrees C of shoot water (first replicate) Peak1Area-Area of melting peak in DSC units (first replicate) Tm-2-Melting point in uncorrected degrees C of shoot water (second replicate) PeakT2-Melting peak in uncorrected degrees C of shoot water (second replicate) Peak2Area-Area of melting peak in DSC units (first replicate) Tm-3-Melting point in uncorrected degrees C of shoot water (third replicate) PeakT3-Melting peak in uncorrected degrees C of shoot water (third replicate) Peak3Area-Area of melting peak in DSC units (first replicate) Tm-mean-Mean melting point in uncorrected degrees C of shoot water -see Calibs.csv for conversion to solute concentration PeakT-mean-Mean melting peak in uncorrected degrees C of shoot water -see Calibs.csv for conversion to solute concentration PeakArea-mean-Mean area of melting peak in DSC units -see Calibs.csv for conversion to water mass Tm-corr-Melting point of shoot water, corrected for calorimetry ramp rate offset PeakT-corr-Melting peak of shoot water, corrected for calorimetry ramp rate offset Dsc-notes-Notes on appearance of differential scanning calorimetry peaks Stem mass-Mass in g of shoot used for microscopy Mean area-Mean area in mm2 of laminar cells (from Moss-meas Datasheet.csv) Sd area-Standard deviation of cell area (from Moss-meas Datasheet.csv) Mean perimeter-Mean perimeter in mm of laminar cells (from Moss-meas Datasheet.csv) Sd perimeter-Standard deviation of cell perimeter (from Moss-meas Datasheet.csv) Mean width-Mean width in mm of laminar cells (from Moss-meas Datasheet.csv)
Sd width-Standard deviation of cell width (from Moss-meas Datasheet.csv) Mean length-Mean length in mm of laminar cells (from Moss-meas Datasheet.csv) Sd length-Standard deviation of cell length (from Moss-meas Datasheet.csv) Micro Mean Fo-Mean Fo of leaves (from Moss-fluorescence micro Datasheet.csv) Micro Sd Fo-Standard deviation Fo of leaves (from Moss-fluorescence micro Datasheet.csv ) Micro Mean Fm-Mean Fm of leaves (from Moss-fluorescence micro Datasheet.csv) Micro Sd Fm- Standard deviation Fm of leaves (from Moss-fluorescence micro Datasheet.csv) Micro Mean Fv.Fm-Mean FvFm of leaves (from Moss-fluorescence micro Datasheet.csv) Micro Sd Fv.Fm- Standard deviation FvFm of leaves (from Moss-fluorescence micro Datasheet.csv)

Moss-fluorescence Datasheet.csv
Date-date of experiment
Run-experimental run (1 to 18)
ClumpSp-dominant species of moss clump: C-Ceratodon purpureus; S- Schistidium antarctici; BC- Bryum pseudotriquetrum and Ceratodon purpureus codominant Replicate-replicate (here equal to Run) Pseudoreplicates-replicates of the same species during the same run Temp-Treatment temperature: 8 degrees C, 16 degrees C or 24 degrees C VPD-Vapour pressure differential of treatment: 0.2kPa, 0.5kPa or 1.2kPa Timepoint-Sampling timepoint (0 = initial conditions) Time-Time in hours since start of experimental run Treatment-Experimental phase: Drying, Recovery (rehydration following drying process), ReRun (second round of drying following recovery) Problem-Identifies if there were any problems with the measurements Fo-Fluorescence in absence of light Fm-Dark adapted maximum fluorescence Fv/Fm-Maximum quantum yield of PSII Fo'1-Minimal light fluorescence (first replicate) Fo'2-Minimal light fluorescence (second replicate) Fo'3-Minimal light fluorescence (third replicate) Fm'1-Maximal light fluorescence (first replicate) Fm'2-Maximal light fluorescence (second replicate) Fm'3-Maximal light fluorescence (third replicate) Mean Fo-Mean Fo of clump Sd Fo-Standard deviation of the Fo of the clump Mean Fm-Mean Fo of clump Sd Fm-Standard deviation of the Fo of the clump Mean Fv.Fm-Mean Fo of clump Sd Fv.Fm-Standard deviation of the Fo of the clump Mean Fo'-Mean Fo of clump Sd Fo'-Standard deviation of the Fo of the clump Mean Fm'-Mean Fo of clump Sd Fm'-Standard deviation of the Fo of the clump

Moss-fluorescence Datasheet.csv
Date-date of experiment
Run-experimental run (1 to 18)
ClumpSp-dominant species of moss clump: C-Ceratodon purpureus; S- Schistidium antarctici; BC- Bryum pseudotriquetrum and Ceratodon purpureus codominant Replicate-replicate (here equal to Run) Temp-Treatment temperature: 8 degrees C, 16 degrees C or 24 degrees C VPD-Vapour pressure differential of treatment: 0.2kPa, 0.5kPa or 1.2kPa Timepoint-Sampling timepoint (0 = initial conditions) Time-Time in hours since start of experimental run Treatment-Experimental phase: Drying, Recovery (rehydration following drying process), ReRun (second round of drying following recovery) RWC stems-Relative water content of shoot in g water per g dry mass (from calorimetry tins) Fo-Fluorescence in absence of light Fm-Dark adapted maximum fluorescence Fv/Fm-Maximum quantum yield of PSII Mean Fo-Mean Fo of clump Sd Fo-Standard deviation of the Fo of the clump Mean Fm-Mean Fo of clump Sd Fm-Standard deviation of the Fo of the clump Mean Fv.Fm-Mean Fo of clump Sd Fv.Fm-Standard deviation of the Fo of the clump

Moss-meas Datasheet.csv
Date-date of experiment
Run-experimental run (1 to 18)
ClumpSp-dominant species of moss clump: C-Ceratodon purpureus; S- Schistidium antarctici; BC- Bryum pseudotriquetrum and Ceratodon purpureus codominant Replicate-replicate (here equal to Run) Temp-Treatment temperature: 8 degrees C, 16 degrees C or 24 degrees C VPD-Vapour pressure differential of treatment: 0.2kPa, 0.5kPa or 1.2kPa Timepoint-Sampling timepoint (0 = initial conditions) Time-Time in hours since start of experimental run Treatment-Experimental phase: Drying, Recovery (rehydration following drying process), ReRun (second round of drying following recovery) RWC-Relative water content of moss clump in g water per g dry mass RWC stems-Relative water content of shoot in g water per g dry mass (from calorimetry tins) Area-Area in mm2 of leaf lamina cell Perimeter-Perimeter in mm of leaf lamina cell Width-Width in mm of leaf lamina cell Length-Length in mm of leaf lamina cell Mean area-Mean area in mm2 of laminar cells Sd area-Standard deviation of cell area Mean perimeter-Mean perimeter in mm of laminar cells Sd perimeter-Standard deviation of cell perimeter Mean width-Mean width in mm of laminar cells Sd width-Standard deviation of cell width Mean length-Mean length in mm of laminar cells Sd length-Standard deviation of cell length

Calib.csv
Solution-Water or sucrose
Bymass-Concentration of sucrose solution in g per g water Concentration-Molar concentration of sucrose solution Volume-Volume in microliters of sample Mass tin-Mass in g of empty calorimetry tin Mass wet-Mass in g of calorimetry tin + sample Mass sample-Mass in g of sample Tm- Melting point in uncorrected degrees C of solution
Peak- Melting peak in uncorrected degrees C of solution
Area- Area of melting peak of solution in DSC units Tf-Freezing point in uncorrected degrees C of solution
Areaf- Area of freezing peak of solution in DSC units


This work was conducted under the auspices of AAS projects 2780 and 2061.

Related URL
Link: VIEW PROJECT HOME PAGE
Description: Public information for ASAC project 2780


Link: VIEW PROJECT HOME PAGE
Description: Public information for ASAC project 3061


Link: VIEW RELATED INFORMATION
Description: Citation reference for this metadata record and dataset


Parent DIF
This data set description is a member of a collection. The collection is described in
AAS_2780


Geographic Coverage
 N: -66.28 S: -66.3  E: 110.52  W: 110.5

Data Set Citation
Dataset Originator/Creator: Stanton, D., Merlin, M., Ball, M. and Bryant, G.
Dataset Title: Climate change and carbon gain in Antarctic mosses - Drying Responses
Dataset Series Name: CAASM Metadata
Dataset Publisher: Australian Antarctic Data Centre
Online Resource: https://data.aad.gov.au/aadc/metadata/metadata_redirect.cfm?md=/AMD...


Temporal Coverage
Start Date: 2012-02-01
Stop Date: 2012-02-29

Start Date: 2012-03-20
Stop Date: 2012-05-24


Location Keywords
CONTINENT > ANTARCTICA
GEOGRAPHIC REGION > POLAR


Science Keywords
BIOLOGICAL CLASSIFICATION >PLANTS >MOSSES/HORNWORTS/LIVERWORTS    [Definition]
BIOSPHERE >ECOLOGICAL DYNAMICS >ECOSYSTEM FUNCTIONS >PHOTOSYNTHESIS    [Definition]
BIOSPHERE >VEGETATION >CHLOROPHYLL    [Definition]


ISO Topic Category
BIOTA


Platform
FIELD INVESTIGATION    [Information]
FIELD SURVEYS    [Information]
LABORATORY    [Information]


Quality
Samples were collected in February 2012. The experiments reported in this dataset were conducted between March 20th 2012 and May 24th 2012.

No major problems were encountered. A small number of chlorophyll measurements are potentially problematic and have been flagged as such in the relevant data sheet and were not included in the final analysis.

The relative proportion of Bryum and Ceratodon in the mixed clumps was variable, and so those clumps were not included in the published final analysis.


Access Constraints
These data are not yet publicly available.

There are two download files, one for data and one containing a copy of a thesis produced from this work.


Use Constraints
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_2780... when using these data.


Keywords
Drying Response
Mass
Water Content
Photosynthesis
cellular sollute
cellular dimensions


Data Set Progress
COMPLETE


Originating Center
Australian Antarctic Division


Data Center
Australian Antarctic Data Centre, Australia    [Information]
Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia



Distribution
Distribution_Media: HTTP
Distribution_Size: 201 kb
Distribution_Format: csv
Fees: Free

Distribution_Media: HTTP
Distribution_Size: 1.4 MB
Distribution_Format: pdf
Fees: Free


Personnel
DANIEL STANTON
Role: INVESTIGATOR
Role: TECHNICAL CONTACT
Phone: +61 2 6125 4558
Email: daniel.stanton at anu.edu.au
Contact Address:
Functional Ecology Group, Ball Lab
Research School of Biology, Plant Sciences Division
Building 46
The Australian National University
City: Canberra
Province or State: ACT
Postal Code: 0200
Country: Australia


MORGANE MERLIN
Role: TECHNICAL CONTACT
Email: merlin at magbio.ens.fr
Contact Address:
Ecole Normale Superieure
City: Paris
Postal Code: 75005
Country: France


MARILYN BALL
Role: INVESTIGATOR
Phone: +61 2 6125 5057
Email: marilyn.ball at anu.edu.au
Contact Address:
Australian National University
Research School of Biology
GPO Box 475
City: Canberra
Province or State: ACT
Postal Code: 2601
Country: Australia


GARY BRYANT
Role: INVESTIGATOR
Phone: +61 3 9925 2139
Email: gary.bryant at rmit.edu.au
Contact Address:
Royal Melbourne Institute of Technology
City: Melbourne
Province or State: Victoria
Postal Code: 3001
Country: Australia



Creation and Review Dates
DIF Creation Date: 2012-09-25
Last DIF Revision Date: 2014-10-09

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