Biodiversity of the nitrogen-fixing microbes of the Antarctic originEntry ID: MARPusm06
Abstract: Nitrogen fixation is essential because it is the only way that organisms can attain nitrogen directly from the atmosphere. Nitrogen-fixing bacteria can exist either in the form of symbiotic relationships with host plants or as free-living nitrogen fixers. In aquatic environments, blue - green alga is an important free-living nitrogen fixer. Cyanobacteria are also one of very few groups of ... organisms that can convert inert atmospheric nitrogen into an organic form, such as nitrate or ammonia.
The specific objectives of the project:
• To isolate and characterize new N2-fixing and denitrification microorganisms and to detect new genetic information involved in nitrogen fixation and denitrification.
• To study the biodiversity denitrification genes using a molecular approach
• To localize the microhabitat of N2-fixing and denitrifying microorganisms in selected Antarctic ecosystems to focus isolation strategies on that size fraction.
Water samples were collected using Wiscosin plankton nets with a 30 μm mesh-size. A total of 40 L of water were filtered through the plankton net. Samples were then preserved with Lugol’s iodine solution and 5% formalin buffer. All samples will be transferred to the laboratory for further cell counting and identification using microscopic examination. Cyanobacterial identification and enumeration from the plankton samples will be carried out.
Data Set Citation
Dataset Originator/Creator: School of Biological Sciences, USM
Dataset Title: nitrogen ixing microbes
Dataset Release Date: not yet
ISO Topic Category
Data Set Progress
Universiti Sains Malaysia
Province or State: Penang
Role: DIF AUTHOR
Email: talha at um.edu.my
Email: talhady at gmail.com
B303, 3RD FLOOR IPS BUILDING UNIVERSITY OF MALAYA
City: KUALA LUMPUR
Postal Code: 50603
Creation and Review Dates
DIF Creation Date: 2009-08-04
Last DIF Revision Date: 2009-08-07