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Record Search Query: [Keyword='viral abundance']
Viral abundance and productivity in the Southern Ocean
Entry ID: BROKE-West_viralabundance

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Summary
Abstract: Data acquisition

Samples were collected at the 3 sites at 9 different depths.
Depths included the bottom depth as well as 8 samples in the top 200M.
50ml samples were collected from the niskin bottles attached to the CTD.
20ml was filtered through a 0.02 microlitre filter to remove the viruses and larger organisms from the sample.
1ml of this virus free water was then pipetted into individual cryovials.
A further 1ml was then taken from the original seawater sample and was then passed through a 0.2 microlitre filter, which retained all the bacteria and algae.
The 0.2 microlitre filter was then placed in the cryovial with the 1ml of virus free seawater.
Samples were then fixed with glutaraldehyde (5 microlitre, 50% concentration) at hourly intervals for 4 hours.
After fixation samples were placed in liquid nitrogen and later stored at -80 degrees C.
All samples will be analysed using a flowcytometer.

The fields in these datasets are:

Date
Leg number
Latitude
Longitude
Subsample
Depth (m)
Bottle Number
Viscosity (cP)

This work was completed as part of ASAC projects 2655 and 2679 (ASAC_2655, ASAC_2679).

Parent DIF
This data set description is a member of a collection. The collection is described in
BROKE-West

Geographic Coverage
 N: -61.0 S: -69.04  E: 30.0  W: 30.0

Temporal Coverage
Start Date: 2006-01-10
Stop Date: 2006-02-28

Location Keywords
OCEAN > SOUTHERN OCEAN
CONTINENT > ANTARCTICA
GEOGRAPHIC REGION > POLAR

Science Keywords
BIOLOGICAL CLASSIFICATION >BACTERIA/ARCHAEA    [Definition]
BIOLOGICAL CLASSIFICATION >PLANTS >MICROALGAE    [Definition]
BIOSPHERE >ECOSYSTEMS >AQUATIC ECOSYSTEMS >PLANKTON    [Definition]

ISO Topic Category
BIOTA
OCEANS

Platform
SHIPS    [Information]
R/V AA >R/V Aurora Australis

Instrument
CTD >Conductivity, Temperature, Depth    [Information]

Quality
Samples were taken at 3 stations along leg 1.
9 depths were sampled at the 3 sites and depths were chosen based on the fluorescence maximum on the downcast of the CTD.

Access Constraints
These data are publicly available from the provided URL.

Use Constraints
This data set conforms to the PICCCBY Attribution License
(http://creativecommons.org/licenses/by/3.0/).

Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-We... when using these data.

Keywords
viral abundance
Viral productivity
southern ocean
Date
Leg number
Latitude
Longitude
Subsample
Depth
Bottle Number
Viscosity

Data Set Progress
COMPLETE

Data Center
Australian Antarctic Data Centre, Australia    [Information]
Data Center URL: http://data.aad.gov.au

Data Center Personnel
Name: DATA OFFICER AADC
Phone: +61 3 6232 3244
Fax: +61 3 6232 3351
Email: metadata at aad.gov.au
Contact Address:
Australian Antarctic Division
203 Channel Highway
City: Kingston
Province or State: Tasmania
Postal Code: 7050
Country: Australia


Distribution
Distribution_Media: HTTP
Distribution_Size: 44 kb
Distribution_Format: Excel, csv
Fees: free

Personnel
DANIEL ASHCROFT
Role: INVESTIGATOR
Role: TECHNICAL CONTACT
Role: DIF AUTHOR

JAMES MITCHELL
Role: INVESTIGATOR
Phone: +61 8 8201 3684
Fax: +61 8 8201 3015
Email: jim.mitchell at flinders.edu.au
Contact Address:
School Of Biological Sciences
GPO BOX 2100
FLINDERS UNIVERSITY OF SA
City: Adelaide
Province or State: South Australia
Postal Code: 5001
Country: Australia

LAURENT SEURONT
Role: INVESTIGATOR
Role: TECHNICAL CONTACT
Phone: +33 3 2199 2937
Email: Laurent.Seuront at univ-lille1.fr
Contact Address:
28 AVENUE FOCH
STATION MARINE DE WIMEREUX
City: WIMEREUX
Postal Code: 62930
Country: France

Related URL
Link: GET DATA
Description: Download point for the data - csv files

Link: GET SERVICE
Description: Download point for the data - CTD Bottle Water Data Index page

Link: VIEW PROJECT HOME PAGE
Description: Public information for ASAC project 2655

Link: VIEW PROJECT HOME PAGE
Description: Public information for ASAC project 2679

Link: VIEW RELATED INFORMATION
Description: Citation reference for this metadata record and dataset

Creation and Review Dates
DIF Creation Date: 2006-03-10

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