A method for recurrent blood sampling of individual birds was perfected before a close study of the behaviour of a number of banded breeding pairs of adelie penguins (Pygoscelis adeliae) was conducted to determine a clear understanding of the hormones circulating in the blood stream in relation to breeding behaviour throughout the entire reproductive cycle. Blood samples were taken from a series of pairs during incubation and chick rearing during nest relief when the two birds of the pair could be sampled. Samples were processed via chromatographic methods for Prolactin.
Peterson, A.J. 1967. Chromatographic determination of steroid hormones from the plasma and gonad extracts of the Adelie penguin [Pygoscelis adeliae]. BSc Hons Pt.III (Physiology ) Project, University of Canterbury, New Zealand.