Fish species of the suborder Notothenioidei were captured in the McMurdo Sound or Terra Nova Bay region and basic biological measurements were taken for all fish (length, weight, gonadosomatic indices, sex, etc). For most species, a variety of gonads covering various developmental stages were collected and fixed for histological work. A number of individuals from 10 species were captured including Pagothenia borchgrevinki, Trematomus pennellii, T. bernacchii, T. nicolai, T. hansoni, T.newnesi, T. eulepidotus, Gymnodraco acuticeps, Chionodraco hamatus and Lyodichthys dearborni.
The specimens are all in existence and effectively none have been destroyed. Fixed tissues are kept at room temperature and frozen tissues are all stored at -80 degrees at Lincoln University, New Zealand. In some cases, the tissues have been used to extract RNA or DNA or proteins samples and these purified samples are also stored at -20 or -80 degree containment freezers.
The raw experimental data is kept in laboratory books at Lincoln University and on computer files, which are backed up 2 times on portable hard drives.
If the samples need to be transeferred from their current location, the receiving lab needs to have the necessary containment certification (i.e. PC2 containment) and transfers have to be done according to MAF protocol. Otherwise the work will need to be completed at Lincoln University. All access is restricted to approval from Dr Victoria Metcalf.