Several individuals of Trematomus bernacchii and Pagothenia borchgrevinki were captured and used in an experiment to understand the ability of these fish to acclimate to increased temperatures. Individuals were thermally acclimated over 24 hours to environmental temperature, 2.5°C and 4°C and over 1 week to 6°C. Fish were maintained at these temperatures for between 0-28 days and their tissues were harvested for subsequent genetic expression analysis to understand changes in gene expression related to thermal acclimation, with a particular emphasis on lipid metabolism and membrane function.
The specimens are all in existence and effectively none have been destroyed. Fixed tissues are kept at room temperature and frozen tissues are all stored at -80 degrees at Lincoln University, New Zealand. In some cases, the tissues have been used to extract RNA or DNA or proteins samples and these purified samples are also stored at -20 or -80 degree containment freezers.
The raw experimental data is kept in laboratory books at Lincoln University and on computer files, which are backed up 2 times on portable hard drives.
If the samples need to be transeferred from their current location, the receiving lab needs to have the necessary containment certification (i.e. PC2 containment) and transfers have to be done according to MAF protocol. Otherwise the work will need to be completed at Lincoln University. All access is restricted to approval from Dr Victoria Metcalf.