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Using labelled antifreeze proteins placed in the gut of notothenioid fish to determine the path they take from the gut to the blood
Entry ID: K066_2000_2003_NZ_1

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Summary
Abstract: The many fish that live in the waters around Antarctica have a unique set of adaptations to cope with the extremely cold conditions in which they live. They have developed antifreeze proteins (AFP) that prevent the fish from freezing by arresting the growth of ice crystals in their tissues. The AFP are produced in the pancreas and released into the gut. It is unknown how the AFP reach the blood. Labelled AFP (with 14C) were placed in the gut of a notothenioid fish Trematomus bernacchii. A series of blood samples was taken and analysed by biological atomic mass spectroscopy (BAMS). There was little evidence to support absorption of AFP from the gut. Labelled AFP was administered with a meal to determine if that affects absorption from the gut. Blood samples were analysed by BAMS.
Geographic Coverage
Spatial coordinates   
  N: -77.85   S: -77.85   E: 166.75   W: 166.75
Min Altitude: 0M Max Altitude: 0M
Min Depth: 5M BELOW SEA LEVEL (BSL) Max Depth: 200M BELOW SEA LEVEL (BSL)

Temporal Coverage
Start Date: 2001-10-01
Stop Date: 2002-12-10
ISO Topic Category
Quality Blood samples and data are still available upon request from the investigator.
Ancillary Keywords
Data Set Progress
COMPLETE
Originating Center
Data Center
Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand Supplemental Info
Data Center URL: http://www.antarcticanz.govt.nz/

Data Center Personnel
Name: SHULAMIT GORDON
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand
Personnel
Role: INVESTIGATOR
Phone: +64 3 479 7570
Fax: +64 3 4797866
Email: craig.marshall at stonebow.otago.ac.nz
Contact Address:
Department of Biochemistry
University of Otago
PO Box 56
Dunedin
Country: NEW ZEALAND
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Creation and Review Dates
DIF Creation Date: 2004-06-02
Last DIF Revision Date: 2009-03-27

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