The extracorporeal loop technique was used to measure oxygen levels of the blood, pre and post-branchial blood pressure, heart rate, blood flow and from these measurements stroke volume of the heart and resistances to blood flow through the gills and the body was calculated. Two species of fish gills were used: Pagothenia borchgrevinki and Trematomus bernacchii. The effects of a number of vasocactive drugs on blood flow and oxygen uptake were determined. The drugs include acetylcholine and its blocker, adrenaline and its blockers, serotonin (5-hydroxytryptamine) and its blockers and adenosine and its blockers.
Forster, M.E. Forster, A.H. Davison, W. 1998 Effects of serotonin, adrenaline and other vasoactive drugs on the branchial blood vessels of the Antarctic fish Pagothenia borchgrevinki. Fish physiology and biochemistry 19(2): 103-109
Gieseg, S.P. Cuddihy, S. Hill, J.V. Davison, W. 2000 A comparison of plasma vitamin C and E levels in two Antarctic and two temperate water fish species. Comparative biochemistry and physiology B 125: 371-378