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The characteristics of an isolated in situ Pagothenia borchgrevinki heart: heart rate and pressure, the effects of drugs, innervation of the heart and biochemical work of the blood and muscle tissue
Entry ID: K057_1996_1997_NZ_3

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Summary
Abstract: The characteristics of an isolated in situ fish heart was investigated. A Pagothenia borchgrevinki fish was killed and its heart was exposed and isolated from its blood supply, but left in place within the fish carcass. Cannulae were inserted through an hepatic vein (input) and ventral aorta (output). These allowed monitoring of rate and pressure and allowed perfusion of ringer. Power and Starling curves were determined, plus the effects of the drugs acetylcholine and adrenaline. The innervation of the heart via the vagus nerve was also investigated. Blood and muscle tissue was collected for biochemical work. Levels of the antioxidants α-tocopherol and vitamin C were measured using HPLC methods and total lipids were extracted from both muscle and blood and analysed.
Related URL
Description: The investigators homepage
Geographic Coverage
Spatial coordinates   
  N: -77.85   S: -77.85   E: 166.75   W: 166.75

Temporal Coverage
Start Date: 1996-11-01
Stop Date: 1996-12-31

Start Date: 1998-11-01
Stop Date: 1998-12-31
ISO Topic Category
Platform
LABORATORY Ancillary Description
Quality The data is in the publications. Please contact the investigator for more information.
Data Set Progress
COMPLETE
Data Center
Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand Supplemental Info
Data Center URL: http://www.antarcticanz.govt.nz/

Data Center Personnel
Name: SHULAMIT GORDON
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand
Personnel
Role: INVESTIGATOR
Phone: +64 3 364 2029
Fax: +64 3 364 2590
Email: bill.davison at canterbury.ac.nz
Contact Address:
School of Biological Sciences
University of Canterbury
Private Bag 4800
City: Christchurch
Country: New Zealand
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Publications/References
Sundin, L. Axelsson, M. Davison, W. Forster, M. 1999 Cardiovascular responses to adenosine in the Antarctic fish Pagothenia borchgrevinki. Journal of experimental biology 202: 2259-2267

Gieseg, S.P. Cuddihy, S. Hill, J.V. Davison, W. 2000 A comparison of plasma vitamin C and E levels in two Antarctic and two temperate water fish species. Comparative biochemistry and physiology B 125: 371-378
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Creation and Review Dates
DIF Creation Date: 2009-03-31
Last DIF Revision Date: 2009-03-31

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