Three soil samples were taken from Hut Point, 73 from Cape Evans and 28 from Cape Royds to determine the presence/absence of Bacillus sp. At Cape Evans, transects were made and soil cores were taken to a depth of approximately 10 cm intervals of 1 meter for the length of the annex area (2003-2004). Other samples included wood, hay and feed. Molecular DNA probes were used to determine if anthrax DNA is present in any of these samples (detection level approximately 3-4 spores per gram dry weight of material). These samples were catalogued for both RNA and DNA extraction to potentially identify expressed genes and possible viability of spores. Absolute quantitative real-time PCR will be utilized for the detection and possible viability of any spores present.
Any person making substantial use of a data set must communicate with the investigators who acquired the data prior to publication and anticipate that the data collectors will be co-authors of published results. This extends to model results and to data organized for retrospective studies.
The data ... available here are intended for scholarly use by the academic and scientific community, with the express understanding that any such use will properly acknowledge the originating investigator. Use or reproduction of any material herein for any commercial purpose is prohibited without prior written permission from the U.S. GLOBEC Data Management Office.