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The effects of pH and drugs on miniature end plate current decay in extraocular muscles of the Antarctic fish Pagothenia borchgrevinki
Entry ID: K012_1982_1983_NZ_2


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Summary
Abstract: Previous experiments indicated that miniature end plate currents (MEPCs) from extraocular muscles of Antarctic fish decay significantly faster than MEPCs from New Zealand temperate fish. As the Antarctic fish contains higher proportion of unsaturated fluid lipids, the fast MEPC decay agrees with the hypothesis relating membrane channel kinetics to membrane viscosity. Recordings of spontaneous MEPCs were made from extraocular muscles of Pagothenia borchgrevinki in order to test the effects of several interacting variables. The effects of pH and drugs on MEPC decay were assessed. MEPCs were recorded in pH 6.55, 7.55 and 8.55 and three drugs were used to test the effects of abundance of neuromuscular transmitter molecules and transmitter sensitive sites and how they may affect the shape of MEPCs. The three drugs were Neostigmine: a cholinesterase inhibitor which would block the inhibitor, Tubocurarine: a receptor blocker which would reduce the number of active receptor sites and Deuterium oxide. The number of quantal doses of acetylcholine released by a nerve to activate a muscle can be estimated by recording repeated responses (end plate potentials (EPPs) to nerve stimulation and calculating mean EPP amplitude and variance. Evoked EPPS were recorded over a range of temperatures (-2°C to 12°C) and was used to estimate the quantal content.
Geographic Coverage
 N: -77.85 S: -77.85  E: 166.75  W: 166.75

Temporal Coverage
Start Date: 1982-10-30
Stop Date: 1982-12-02


Location Keywords
CONTINENT > ANTARCTICA
GEOGRAPHIC REGION > POLAR


Science Keywords
AGRICULTURE >ANIMAL SCIENCE >ANIMAL PHYSIOLOGY AND BIOCHEMISTRY    [Definition]
BIOLOGICAL CLASSIFICATION >ANIMALS/VERTEBRATES >FISH >RAY-FINNED FISHES
BIOLOGICAL CLASSIFICATION >ANIMALS/VERTEBRATES >FISH >RAY-FINNED FISHES >PERCH-LIKE FISHES
BIOSPHERE >ECOLOGICAL DYNAMICS >SPECIES/POPULATION INTERACTIONS >EVOLUTIONARY ADAPTATION    [Definition]


ISO Topic Category
BIOTA


Platform
LABORATORY    [Information]
FIELD INVESTIGATION    [Information]


Keywords
Notothenioid
miniature end plate currents
neuromuscular transmitter molecules
transmitter sensitve sites
extra ocular muscles


Data Set Progress
COMPLETE


Data Center
Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand    [Information]
Data Center URL: http://www.antarcticanz.govt.nz/

Data Center Personnel
Name: SHULAMIT GORDON
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand


Personnel
JOHN MACDONALD
Role: INVESTIGATOR
Phone: +64 9 423 9387
Email: ja.macdonald at xtra.co.nz
Contact Address:
Omiha Farm
City: Wellsford
Postal Code: 0972
Country: NEW ZEALAND


RUFUS WELLS
Role: INVESTIGATOR
Phone: +64 9 373 7599 xt 87206
Fax: +64 9 373 7415
Email: r.wells at auckland.ac.nz
Contact Address:
School of Biological Sciences
University of Auckland
Private Bag 92019
Auckland Mail Centre
City: Auckland
Postal Code: 1142
Country: NEW ZEALAND


JOHN MONTGOMERY
Role: INVESTIGATOR
Phone: +64 9 373 7599 xt 83611
Fax: +64 9 422 6113
Email: j.montgomery at auckland.ac.nz
Contact Address:
Leigh Marine Laboratory
University of Auckland
Private Bag 92019
Auckland Mail Centre
City: Auckland
Postal Code: 1142
Country: NEW ZEALAND


CEISHA POIROT
Role: DIF AUTHOR
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: c.poirot at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand


SHULAMIT GORDON
Role: DIF AUTHOR
Phone: +64 3 358 0200
Fax: +64 3 358 0211
Email: s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745
City: Christchurch
Country: New Zealand


Publications/References
Macdonald, J.A. 1983. Neuromuscular cold adaptation in an Antarctic fish. NZ Antarctic Record 5(2): 1-10.

Macdonald, J.A. 1983. Effect of lipid composition on synaptic currents. Proc International Union Physiol Science 15: 413.

Macdonald, J.A. Montgomery, J.C. Wells, R.M.G. Comparative physiology of Antarctic fishes. Advances in marine biology 24: 321-388, 1987.

Macdonald, J.A. Comparative physiology of Antarctic fish. New Zealand Antarctic record 4(3): 17, 1983.

Macdonald, J.A. Montgomery, J.C. Wells, R.M.G. The physiology of McMurdo Sound fishes: current New Zealand research. Comparative biochemistry and physiology 90B(3): 567-578, 1988.

Cox, S.L. and Macdonald, J.A. 2008. Temperature dependency of miniature end plate currents from the extraocular muscle of Antarctic teleost fishes. Polar biology 31 (6): 709-715.

Creation and Review Dates
DIF Creation Date: 2009-01-08
Last DIF Revision Date: 2009-01-09


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