Summary Abstract: Previous experiments indicated that miniature end plate currents (MEPCs) from extraocular muscles of Antarctic fish decay significantly faster than MEPCs from New Zealand temperate fish. As the Antarctic fish contains higher proportion of unsaturated fluid lipids, the fast MEPC decay agrees with the hypothesis relating membrane channel kinetics to membrane viscosity. Recordings of spontaneous MEPCs were made from extraocular muscles of Pagothenia borchgrevinki in order to test the effects of several interacting variables. The effects of pH and drugs on MEPC decay were assessed. MEPCs were recorded in pH 6.55, 7.55 and 8.55 and three drugs were used to test the effects of abundance of neuromuscular transmitter molecules and transmitter sensitive sites and how they may affect the shape of MEPCs. The three drugs were Neostigmine: a cholinesterase inhibitor which would block the inhibitor, Tubocurarine: a receptor blocker which would reduce the number of active receptor sites and Deuterium oxide. The number of quantal doses of acetylcholine released by a nerve to activate a muscle can be estimated by recording repeated responses (end plate potentials (EPPs) to nerve stimulation and calculating mean EPP amplitude and variance. Evoked EPPS were recorded over a range of temperatures (-2°C to 12°C) and was used to estimate the quantal content.

Geographic Coverage

N: -77.85

S: -77.85

E: 166.75

W: 166.75

Temporal Coverage

Start Date: 1982-10-30

Stop Date: 1982-12-02

Location Keywords

CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR

Science Keywords

AGRICULTURE >ANIMAL SCIENCE >ANIMAL PHYSIOLOGY AND BIOCHEMISTRY    [Definition]

BIOLOGICAL CLASSIFICATION >ANIMALS/VERTEBRATES >FISH >RAY-FINNED FISHES

BIOLOGICAL CLASSIFICATION >ANIMALS/VERTEBRATES >FISH >RAY-FINNED FISHES >PERCH-LIKE FISHES

BIOSPHERE >ECOLOGICAL DYNAMICS >SPECIES/POPULATION INTERACTIONS >EVOLUTIONARY ADAPTATION    [Definition]

ISO Topic Category

BIOTA

Platform

LABORATORY    [Information]

FIELD INVESTIGATION    [Information]

Keywords

Notothenioid

miniature end plate currents

neuromuscular transmitter molecules

transmitter sensitve sites

extra ocular muscles

Data Set Progress

COMPLETE

Data Center

Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand    [Information] Data Center URL: http://www.antarcticanz.govt.nz/ Data Center Personnel Name: SHULAMIT GORDON Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: s.gordon at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand

Personnel

JOHN MACDONALD Role: INVESTIGATOR Phone: +64 9 423 9387 Email: ja.macdonald at xtra.co.nz Contact Address: Omiha Farm City: Wellsford Postal Code: 0972 Country: NEW ZEALAND

RUFUS WELLS Role: INVESTIGATOR Phone: +64 9 373 7599 xt 87206 Fax: +64 9 373 7415 Email: r.wells at auckland.ac.nz Contact Address: School of Biological Sciences University of Auckland Private Bag 92019 Auckland Mail Centre City: Auckland Postal Code: 1142 Country: NEW ZEALAND

JOHN MONTGOMERY Role: INVESTIGATOR Phone: +64 9 373 7599 xt 83611 Fax: +64 9 422 6113 Email: j.montgomery at auckland.ac.nz Contact Address: Leigh Marine Laboratory University of Auckland Private Bag 92019 Auckland Mail Centre City: Auckland Postal Code: 1142 Country: NEW ZEALAND

CEISHA POIROT Role: DIF AUTHOR Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: c.poirot at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand

SHULAMIT GORDON Role: DIF AUTHOR Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: s.gordon at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand

Creation and Review Dates

DIF Creation Date: 2009-01-08 Last DIF Revision Date: 2009-01-09

Reformat as FGDC document

[ Update this Record ]

Updating this record requires registration. (Account holders) Update this Record Need an account?   close