Unique physiological and metabolic properties of Arctic mosses are responsible for their acclimation to the inclement polar environment. To perform transcriptome analysis of an Arctic moss species adapted to polar conditions, we constructed a complementary DNA (cDNA) library using total high-quality RNA extracted from the moss species Aulacomnium turgidum. The library consisted of 1.81 x 106 of independent clones with 97.41% of recombinants. A total of 509 cDNA clones were sequenced. After eliminating poor quality sequences, vector trimming and clustering, 360 unigenes consisting of 33 contigs and 327 singletons were identified.
Complementary DNA library construction and expressed sequence tag analysis of an Arctic moss, Aulacomnium turgidum.