Estimating genetic variation is important in understanding a populations potential to track changing environmental conditions and for conservation management. Two molecular techniques, DNA sequencing and microsatellite markers, were used to determine the genetic structure of and relationships amoung moss populations. A phylogenetic analysis of ITS sequence variation was conducted, focusing on the genuses Bryum and Hennediella and moss specific microsatellite markers were used to address questions of population variability as well as taxonomic delimitations and phylogenetic relationships. Samples of mosses were obtained from Cape Bird (25-29 January 2000), Beaufort Island (28 January, 2000), Miers Valley (30 January - 3 February, 2000), Cape Crozier (11-12 January, 2001), Marble Point (12-16 January, 2001) and Granite Harbour (16-19 January, 2001). Additional samples for comparison were obtained from Australia, Sub-Antarctic Islands and other Southern Hemisphere localities.
The moss samples (and the DNAs) that remain are currently stored at Massey University, Palmerston North, New Zealand. The investigator is collaborating with two authors on another molecular systematics papers utilizing these samples. Until this work is completed we will not be able to allow others to access the samples.
+64 3 358 0200
+64 3 358 0211
s.gordon at antarcticanz.govt.nz
Antarctica New Zealand
Private Bag 4745 City:
+64 7 838 4053
+64 7 838 4324
c.gemmill at waikato.ac.nz
Department of Biological Sciences
University of Waikato
PO Box 3105 City:
Stevens, M., Hunger, S., Hills, S. and Gemmill, C. 2007. Phantom hitch-hikers mislead estimates of genetic variation in Antarctic mosses. Plant Systematics and Evolution 263: 191-201.
Hunger, S.A. A study on molecular systematics of the Antarctic Bryum species and the development of microsatellite DNA markers in Bryum argenteum Hedw. M.Sc., University of Waikato : 2000.
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