Genetic sequencing of terrestrial invertebrates along the latitudinal range of the Ross Dependency to determine patterns of biodiversity, phylogenetic relationships and levels of gene flow among populations Entry ID:
K028_1999_2008_NZ_1
Abstract:
Molecular techniques (allozyme electrophoresis and mitochondrial gene sequencing) were used to characterise the Antarctic terrestrial biodiversity along the latitudinal range of the Ross Dependency to assess existing levels of biodiversity and to provide information on the origin, evolutionary relationships and present day dispersal patterns of Antarctic invertebrate taxa. Arthropods (springtails and mites) nematodes, rotifers and tardigrades were collected from Ross Island (Cape Evans, Cape Royds, Cape Bird, Cape Crozier, Observation Hill, around Scott Base), Cape Hallett, Granite Harbour (Cape Geology, Botany Bay, Mt England, Flatiron, Mt Suess and Sperm Bluff), Beaufort Island, Marble Point, the ice free areas of Wright and Victoria Valley’s, the Taylor Valley, the Miers Valley, the Garwood Valley, Lake Penny, Lake Morning and Minna Bluff, the Darwin Glacier area (mites and nematodes only from the Brown Hills region, the base of the Foggydog Glacier and the eastern end of Diamond Hill) and Mt Kyffin.
The springtails (Collembola) species collected include Desoria klovstadi, Fresia grisea, Cryptopygus cisantarcticus, Gomphiocephalus hodgsoni, Antarctophorus sudpolaris, Biscoia subpolaris and Neocryptopygus nivicolis. The mite (Acari) species collected were Stereotydeus mollis and Nanorchestes spp. Some genetic work was completed on one species of nematode Scottnema lindsayae from Mt Kyffin Traditional, morphologically-based, taxonomic approaches for assessing biodiversity were combined with more recent molecular techniques (e.g. allozyme and DNA analyses) with individuals from each study site evaluated using protein electrophoresis, mtDNA and morphological analyses. Allozyme electrophoresis was used to determine genetic differences within and among populations. Genotype frequencies were recorded for each locus at each site. Detailed and quantitative observations were made on modes of dispersal through the use of windsocks, pitfall traps and stream drift nets. These analyses are designed to accurately assess existing levels of biodiversity and to provide information on the origin, evolutionary relationships and present day dispersal patterns of Antarctic invertebrate taxa. It could also be used in understanding a species ability to track environmental changes and defining units for conservation management. This programme will improve our knowledge of the Antarctic terrestrial fauna and provide information for the better management and conservation of Antarctic terrestrial habitats.
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Link:
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Description:
The unique genetic sequences of springtails and mites are loaded into the international database GENBANK.
Name:
SHULAMIT
GORDON Phone:
+64 3 358 0200
Fax:
+64 3 358 0211
Email:
s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745 City:
Christchurch
Country:
New Zealand
Personnel
IAN
HOGG Role:
INVESTIGATOR
Phone:
+64 7 838 4225
Fax:
+64 4 838 4324
Email:
hogg at waikato.ac.nz
Contact Address:
Department of Biological Sciences
University of Waikato
Private Bag 3105 City:
Hamilton
Country:
NEW ZEALAND
CEISHA
POIROT Role:
DIF AUTHOR
Phone:
+64 3 358 0200
Fax:
+64 3 358 0211
Email:
c.poirot at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745 City:
Christchurch
Country:
New Zealand
SHULAMIT
GORDON Role:
DIF AUTHOR
Phone:
+64 3 358 0200
Fax:
+64 3 358 0211
Email:
s.gordon at antarcticanz.govt.nz
Contact Address:
Antarctica New Zealand
Private Bag 4745 City:
Christchurch
Country:
New Zealand
Publications/References
Caruso, T., Hogg, I.D., Carapelli, A., Frati, F., Bargagli, R. Large-scale spatial patterns in the distribution of Collembola (Hexapoda) species in Antarctic terrestrial ecosystems. Journal of biogeography 36: 879-886 2009.
Stevens, M.I. Population genetic structures and dispersal patterns of arthropods in New Zealand and the Ross Dependency, Antarctica. Ph.D., University of Waikato : 2002
Stevens, M.I. Hogg, I.D. Expanded distributional records of Collembola and Acari in southern Victoria Land, Antarctica. Pedobiologia 46: 485-495, 2002.
Hogg, I.D. Stevens, M.I. Soil Fauna of Antarctic Coastal Landscapes. In: Geoecology of Antarctic ice-free coastal landscapes.Beyer L. Bölter M(eds) Berlin Heidelberg: Springer-Verlag. 2002. pp.265-280 [Ecological Studies, Vol. 154]
Stevens, M.I. Hogg, I.D Long-term isolation and recent expansion from glacial refugia revealed for the endemic springtail Gomphiocephalus hodgsoni from Victoria Land, Antarctica. Molecular ecology 12: 2357-2369, 2003.
Stevens, M.I. Greenslade, P. Hogg, I.D. Sunnucks, P. Southern Hemisphere springtails: could they have survived glaciation of Antarctica? Molecular biology and evolution 23(5): 874-882, 2005.
McGaughran, A. Genetic diversity and phylogeography of New Zealand and Antarctic arthropods. M.Sc., University of Waikato : 2005.
Hogg, I.D. Cary, S.C. Convey, P. Newsham, K.K. O’Donnell, A.G. Adams, B.J. Aislabie, J. Frati, F. Stevens, M.I. Wall, D.H. Biotic interactions in Antarctic terrestrial ecosystems: Are they a factor? Soil biology and biochemistry 38(10): 3035-3040 doi:10.1016/j.soilbio.2006.04.026, 2006.
Nolan, L. Hogg, I.D. Stevens, M.I. Haase, M. Fine scale distribution of mtDNA haplotypes for the springtail Gomphiocephalus hodgsoni (Collembola) corresponds to an ancient shoreline in Taylor Valley, continental Antarctica. Polar biology 29: 813-819doi 10.1007/s00300-006-0119-4, 2006.
Stevens, M.I. Fjellberg, A. Greenslade, P. Hogg, I.D. Sunnucks, P. Redescription of the Antarctic springtail Desoria klovstadi using morphological and molecular evidence. Polar biology : doi:10.1007/s003000-006-0120-y, 2006.
Stevens, M.I. Hogg, I.D. Contrasting levels of mitochondrial DNA variability between mites (Penthalodidae) and springtails (Hypogastruridae) from the Trans-Antarctic Mountains suggest long-term effects of glaciation and life history on substitution rates, and speciation processes. Soil biology and biochemistry 38(10): 3171-3180 doi:10.1016/j.soilbio.2006.01.009, 2006.
Stevens, M.I. Hogg, I.D. The molecular ecology of Antarctic terrestrial and limnetic invertebrates and microbes. In: Trends in Antarctic terrestrial and limnetic ecosystems: Antarctica as a global indicator.Bergstrom D.M. Convey P. Huiskes A.H.(eds.) Dordrecht, The Netherlands: Springer. 2006. pp. ISBN 1-4020-5276-6
Stevens, M.I. Frati, F. McGaughran, A. Spinsanti, G. Hogg, I. Phylogeographic structure suggests multiple glacial refugia in northern Victoria Land for the endemic Antarctic springtail Desoria klovstadi (Collembola, Isotomidae).Zoologica scripta 36(2): 201-212, 2007.
Adams, B.J. Wall, D.H. Gozel, U. Dillman, A.R. Chaston, J.M. Hogg, I.D. The southernmost worm, Scottnema lindsayae (Nematoda): diversity, dispersal and ecological stability. Polar biology 30: 809-815 doi: 10.1007/s00300-006-0241-3, 2007.
Convey, P. Stevens, M.I. Antarctic biodiversity. Science 317: 1877-1878, 2007.
Adams, B.J., Bardgett, R.D., Ayres, E., Wall, D.J., Aislabie, J., Bamforth, S., Bargagli, R., Cary, C., Cavacini, P., Connell, L., et al. 2006. Diversity and distribution of Victoria Land biota. Soil biology and biochemistry 38(10): 3003-3018.
McGaughran, A., Hogg, I.D., Stevens, M.I. 2008. Patterns of population genetic structure for springtails and mites in southern Victoria Land, Antarctica. Molecular phylogenetics and evolution 46: 606-618. doi:10.1016/j.ympev.2007.10.003
McGaughran, A., Torricelli, G., Carapelli, A., Frati, F., Stevens, M.I., Convey, P., Hogg, I.D. 2009. Contrasting phylogeographical patterns for springtails reflect different evolutionary histories between the Antarctic Peninsula and continental Antarctica. Journal of biogeography. doi:10.1111/j.1365-2699.2009.02178.x
McGaughran, A. 2009. Polar evolution: molecular genetic and physiological parameters of Antarctic arthropod populations. PhD Thesis, Massey University. pp. 264
Creation and Review Dates
DIF Creation Date:
2005-05-22
Last DIF Revision Date:
2010-02-23
