Summary Abstract: A taxonomic assessment of sea ice bacteria abundance and their response to climate induced stress was investigated from the sea ice around Seabee Hook, Cape Hallett. Four sites at different points around Seabee Hook were established for a representative survey of the annual ice present in Edisto Inlet. The ice had broken out the previous year and new ice formed over the winter providing ideal conditions to compare the distribution and abundance of bacteria occurring in pack ice at the tongue of the Mertz Glacier with bacteria from fast ice at Cape Hallett. Bacteria were collected from the ice at regular intervals with ice cores. A complete ice profile was extracted at each site to examine community composition and structure. Water samples were also collected from 5, 25 and 50m depths and processed in the same way as ice cores and compared. In the lab, bacterial samples were grown under light and dark conditions. Epi-fluorescence microscopy was used to analyse the metabolic activity of bacterial cells by CTC staining (which reveals metabolically active cells), DAPI staining (total cell numbers), VSP (Vital Stain and Probe which utilises oligonucleotide probes to distinguish metabolic activity), DVC (Direct Viable Count - which indicates whether cells are capable of undergoing division) and VIAGRAM (which distinguishes between live and dead cells and also gram positive and gram negative cells). The results were examined using laser based technology (flow cytometry) and epi-fluorescence microscopy. If bacteria do respond to light, one possible explanation is that they respond to increased levels of algal photosynthate. Sugars produced by photosynthesis may be released into the seawater and then be taken up by bacterial cells. To test this idea, 14CO2 was fed to sea ice algae and then they were incubated in the light at -2 °C. The algal cells were removed and the filtrate containing labelled photosynthate was fed to sea ice bacteria. Samples were analysed by a Liquid Scintillation counter.

Related URL

Link: GET DATA Description: Andrew Martin MSc pdf Link: GET DATA Description: Andrew Martin PhD pdf Link: VIEW RELATED INFORMATION Description: Investigators research page

Geographic Coverage

N: -72.3166

S: -72.3166

E: 170.2666

W: 170.2666

Temporal Coverage

Start Date: 2003-11-19

Stop Date: 2003-12-16

Start Date: 2004-11-12

Stop Date: 2004-12-07

Location Keywords

CONTINENT > ANTARCTICA CONTINENT > ANTARCTICA > CAPE HALLETT CONTINENT > ANTARCTICA > SEABEES HOOK GEOGRAPHIC REGION > POLAR

Science Keywords

TERRESTRIAL HYDROSPHERE >WATER QUALITY/WATER CHEMISTRY >CHLOROPHYLL    [Definition]

BIOLOGICAL CLASSIFICATION >BACTERIA/ARCHAEA    [Definition]

BIOLOGICAL CLASSIFICATION >PLANTS >MICROALGAE    [Definition]

BIOSPHERE >AQUATIC ECOSYSTEMS >PLANKTON >PHYTOPLANKTON    [Definition]

BIOSPHERE >AQUATIC ECOSYSTEMS >MARINE HABITAT    [Definition]

BIOSPHERE >ECOLOGICAL DYNAMICS >COMMUNITY DYNAMICS >BIODIVERSITY FUNCTIONS    [Definition]

BIOSPHERE >ECOLOGICAL DYNAMICS >ECOSYSTEM FUNCTIONS >PHOTOSYNTHESIS    [Definition]

BIOSPHERE >ECOLOGICAL DYNAMICS >ECOSYSTEM FUNCTIONS >BIOMASS DYNAMICS    [Definition]

BIOSPHERE >ECOLOGICAL DYNAMICS >ECOSYSTEM FUNCTIONS >PRIMARY PRODUCTION    [Definition]

ISO Topic Category

BIOTA OCEANS

Platform

FIELD SURVEYS    [Information]

LABORATORY    [Information]

FIELD INVESTIGATION    [Information]

Project

LGP >Latitudinal Gradient Project    [Information]

EBA >Evolution and Biodiversity in the Antarctic    [Information]

Quality

The data is contained in the thesis. Existing samples are held at Victoria University of Wellington with a spreadsheet of sample numbers. All raw data is held by the investigator. Please contact Dr Ken Ryan for more information.

Data Set Progress

COMPLETE

Data Center

Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand    [Information] Data Center URL: http://www.antarcticanz.govt.nz/ Data Center Personnel Name: SHULAMIT GORDON Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: s.gordon at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand

Personnel

KEN RYAN Role: INVESTIGATOR Phone: +64 4 463 6083 Fax: +64 4 463 5331 Email: ken.ryan at vuw.ac.nz Contact Address: Victoria University of Wellington School of Biological Sciences PO BOX 600 City: Wellington Postal Code: 6140 Country: New Zealand

SHULAMIT GORDON Role: DIF AUTHOR Phone: +64 3 358 0200 Fax: +64 3 358 0211 Email: s.gordon at antarcticanz.govt.nz Contact Address: Antarctica New Zealand Private Bag 4745 City: Christchurch Country: New Zealand

Creation and Review Dates

DIF Creation Date: 2005-09-22 Last DIF Revision Date: 2010-02-23

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