Abstract: As part of NASA's Accelerated Canopy Chemistry Program (ACCP) analyses were performed for the determination of carbon constituents and nitrogen content in fresh forest foliage. Samples were analyzed using a series of extractions that yielded different carbon constituents: non-polar, polar, cellulose and lignin. Nitrogen analyses were conducted using a standard combustion procedure. Approximately 1000 leaf samples were collected from 5 geographically distinct sites and were analyzed at the University of New Hampshire to ensure consistency in analysis. Results were used as a calibration set for Visible/NIR reflectance and the estimation of canopy carbon and nitrogen concentrations.