X-ray diffraction data using ice-binding protein (LeIBP) crystal
Entry ID: KPDC_X-rayDUIB_LeIBP_2012

[ Update this Record ]

Summary
Abstract: Psychrophilic Arctic yeast Leucosporidium sp. produces a glycosylated ice-binding protein (LeIBP) with a molecular mass of approximately 25 kDa, which can lower the freezing point below the melting point once it binds to ice. LeIBP exhibits low amino acid sequence similarity to other antifreeze proteins with known protein structures. Recently, we developed an expression system allowing high-level production and efficient purification of recombinant pLeIBP. Furthermore, crystallization and preliminary X-ray crystallographic analysis of the ice-binding protein were performed.

Purpose: To investigate the antifreeze mechanism of LeIBP, we have carried out structural studies. As the first step toward its structural elucidation, we report the results of preliminary X-ray crystallographic experiments with LeIBP.
ISO Topic Category
Instrument
CORING DEVICES Ancillary Description
Originating Center
Data Center
Korea Polar Research Institute, Republic of Korea Supplemental Info
Data Center URL: http://www.kopri.re.kr/

Data Center Personnel
Name: MIN CHOUL LEE
Phone: +82-32-260-6081
Fax: +82-32-260-6088
Email: leemc at kopri.re.kr
Contact Address:
Songdo Techno Park, 7-50, Songdo-dong, Yeonsu-gu
City: Incheon
Postal Code: 406-840
Country: Korea


Data Center Personnel
Name: JAE LIM KIM
Phone: +82-260-6083
Fax: +82-260-6088
Email: kabuli at kopri.re.kr
Contact Address:
Songdo Techno Park, 7-50, Songdo-dong, Yeonsu-gu
City: Incheon
Postal Code: 406-840
Country: Korea
Personnel
Role: DIF AUTHOR
Role: TECHNICAL CONTACT
Phone: +82-32-260-6351
Email: junhyucklee at kopri.re.kr
Contact Address:
Songdo Techno Park, 7-50, Songdo-dong, Yeonsu-gu
City: Incheon
Postal Code: 406-840
Country: Korea
Extended Metadata Properties
(Click to view more)

Creation and Review Dates
DIF Creation Date: 2013-05-06
Last DIF Revision Date: 2013-05-13
[ Update this Record ]


Link to Web Site