Using microsatellites as markers to explore genetic variation in Ceratodon purpureus

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: Using microsatellites as markers to explore genetic variation in Ceratodon purpureus
Description:
Abstract:
Sampling records for Project 2542 Moss sampling in the Windmill Islands and the Vestfold Hills during 2005 for genetic analysis. Samples are stored frozen at the University of Wollongong and will be analysed using microsatellites. Collections of the mosses Ceratodon purpureus and Grimmia antarctici from the Windmill islands region and the Vestfold Hills. Sites include Clark Peninsula, Antarctic Specially Protected Areas 135 and 136 (formerly known as SSSI16 and SSSI 17), Robinson's Ridge, Peterson Island and around Casey station in the Windmill islands, and Mossel Lake, Lichen Valley and Grimmia Gorge in the Vestfold Hills. Windmill Islands collections 11-14 January 2005. Vestfold Hills collections 20-21 January 2005. Samples were collected with tweezers, with each sample containing 5-10 shoots. Samples will be used to survey the genetic variation in these mosses in the Antarctic. Notes about the dataset: Random, medium and fine refer to the scale of sampling used. Random samples were a single sample (tweezer pinch) of moss was taken from that GPS point. Medium scale samples used a 20 x 20 cm quadrat to collect samples at each of the corners and the centre of the quadrat, with a total of five samples for the GPS position. For fine scale samples, a small clump of shoots, with approximately 2 cm square of shoot tips each, were collected, so once again were only a single collection per position, except for the fine scale sampling at Robinson's Ridge, in which 25 samples were taken within a 5 x 5 cm quadrat. M8 marker etc. refer to various markers within or around the moss beds from previous or ongoing studies (by other researchers). These were recorded as additional guides for relocating sample sites if need be. 'Z pattern' refers to the order of sample collection within the quadrat. The fields in this dataset are: Sample Number Station Species Location Latitude Longitude Sampling Notes Quadrats Sites Two new spreadsheets were added in October, 2007. The following notes apply to these spreadsheets. 'metadata windmill is.xls' 'metadata all Ceratodon genotypes.csv' Quadrat and independent labels are used in some instances instead of 'fine, medium and random' that have been previously used. Quadrat and independent are equivalent to the medium and random sampling in previous spreadsheets. Fine scale samples weren't genotyped. Each of the five columns F-J (in both spreadsheets, in 'metadata windmill is.xls' it is the 'genotypes' sheet) are a different microsatellite locus for the moss Ceratodon purpureus (e.g. CEPU105), with the numbers in each row indicating the microsatellite allele or genotype as the length in DNA base pairs in that sample. Multilocus genotype ('metadata windmill is.xls' spreadsheet, 'genotypes and GPS' worksheet) is the combination of each of the 5 microsatellite alleles, if two samples share the same 5 alleles they have the same multilocus genotype. The GenAlEx program (this is the input for a population genetics program) gives a letter for genotypes present in more than one sample, and a number for genotypes only present in one sample, so 'E' is a very common multilocus genotype in this data set. In February 2011, two more documents were added. A csv spreadsheet detailing locations of mosses found in 2005, and a dating word document.
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Dunn J.L., Robinson S.A. 2006 Ultraviolet B screening potential is higher in two cosmopolitan moss species than in a co-occurring Antarctic endemic moss: implications of continuing ozone depletion. Global Change Biology 12 2282-2296
Supplemental_Information:
REFERENCE: Hennion F., Huiskes A.H.L., Robinson S., Convey P. 2006 Physiological traits of organisms in a changing environment. Bergstrom D.M., Convey P., Huiskes A.H.L. Trends in Antarctic Terrestrial and Limnetic Ecosystems: Antarctica as a Global Indicator. 127-157
Supplemental_Information:
REFERENCE: Dunn J.L., Robinson S.A. 2006 UV-B screening potential is higher in two cosmopolitan moss species than in a co-occurring Antarctic endemic moss - implications of continuing ozone depletion Global Change Biology 12 12 42
Supplemental_Information:
REFERENCE: Clarke, LJ, Robinson, SA and Ayre, DJ 2009 Genetic structure of Antarctic populations of the moss Ceratodon purpureus. Antarctic Science 21 51-58
Supplemental_Information:
REFERENCE: Clarke, LJ, Robinson, SA and Ayre, DJ 2008 Somatic mutation and the Antarctic ozone hole Journal of Ecology 96 378-385
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20050111
Ending_Date: 20050114
Currentness_Reference:Unknown
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20050120
Ending_Date: 20050121
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 72.0
East_Bounding_Coordinate: 72.5
North_Bounding_Coordinate: -68.0
South_Bounding_Coordinate: -68.5
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 110.0
East_Bounding_Coordinate: 110.5
North_Bounding_Coordinate: -66.0
South_Bounding_Coordinate: -66.5
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: PROJECT
Theme_Keyword_Thesaurus: ANCILLARY KEYWORDS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MOSSES/HORNWORTS/LIVERWORTS
Theme_Keyword: FIELD SURVEYS
Theme_Keyword: GROUND-BASED OBSERVATIONS
Theme_Keyword: LABORATORY
Theme_Keyword: FIELD INVESTIGATION
Theme_Keyword: EBA > EVOLUTION AND BIODIVERSITY IN THE ANTARCTIC
Theme_Keyword: Latitude
Theme_Keyword: Location
Theme_Keyword: Longitude
Theme_Keyword: Notes
Theme_Keyword: Quadrats
Theme_Keyword: Sample Number
Theme_Keyword: Sampling
Theme_Keyword: Sites
Theme_Keyword: Species
Theme_Keyword: Station
Theme_Keyword: BIOTA
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA > VESTFOLD HILLS
Place_Keyword: CONTINENT > ANTARCTICA > WINDMILL ISLANDS
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: The data are publicly available for download from the URL given below or in the data centre database or GBIF. The dating data, however, are not yet publicly available.
Use_Constraints:
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2542 when using these data.
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: SHARON ROBINSON
Contact_Position: INVESTIGATOR
Contact_Position: TECHNICAL CONTACT
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Biological Sciences
Address: University of Wollongong
Address: Northfields Ave
City: WOLLONGONG
State_or_Province: New South Wales
Postal_Code: 2522
Country: Australia
Contact_Voice_Telephone: +61 2 4221 5753
Contact_Facsimile_Telephone: +61 2 4221 4135
Contact_Electronic_Mail_Address: sharonr@uow.edu.au
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
The latitudes and longitudes provided in spatial coverage are approximate only.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: AU/AADC > Australian Antarctic Data Centre, Australia
Contact_Person: DATA OFFICER AADC
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +61 3 6232 3244
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: metadata@aad.gov.au
Resource_Description: ASAC_2542
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: csv, excel
Transfer_Size: 21 kb
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/portal/download_file.c...
Access_Instructions:
Download point for the data - csv files and excel spreadsheet
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/portal/download_file.c...
Access_Instructions:
Download point for the dating data
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
https://secure3.aad.gov.au/proms/public/projects/r...
Access_Instructions:
Public information for ASAC project 2542
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/metadata/citation.cfm?...
Access_Instructions:
Citation reference for this metadata record and dataset
Fees: Free
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Metadata_Reference_Information:
Metadata_Date: 20050302
Metadata_Review_Date: 20140110
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: SHARON ROBINSON
Contact_Position: INVESTIGATOR
Contact_Position: TECHNICAL CONTACT
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Biological Sciences
Address: University of Wollongong
Address: Northfields Ave
City: WOLLONGONG
State_or_Province: New South Wales
Postal_Code: 2522
Country: Australia
Contact_Voice_Telephone: +61 2 4221 5753
Contact_Facsimile_Telephone: +61 2 4221 4135
Contact_Electronic_Mail_Address: sharonr@uow.edu.au
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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