Investigation of the role of anti-freeze glyco-proteins in the circulation system of Antarctic fish

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: Investigation of the role of anti-freeze glyco-proteins in the circulation system of Antarctic fish
Description:
Abstract:
In order to investigate how Antarctic fish deal with ice in their blood circulatory system, Trematomus bernacchii and Pagothenia borchgrevinki Antarctic fish were captured for experimental investigation. A variety of biochemical techniques were used to isolate Anti-Freeze Glyco-Proteins (AFGPs) from Antarctic fish blood, which was then conjugated directly to a fluorescent marker. We synthesised silicon dioxide nanobeads incorporating a fluorescent probe and coupled these to AFGP via a multimeric linking protein or dendrimer based on G4.5 poly(amidoamine). These modified nanobeads, consisting of a fluorescent particle surrounded by AFGPs, were designed to serve as a proxy for ice crystals that have bound AFGPs. When introduced into the circulation of the fish, we were able to follow the fate of free fluorescent AFGP and particle-bound fluorescent AFGP (the latter serving as a proxy for ice in the circulation). Frozen and alcohol-fixed tissues were returned to New Zealand for further laboratory analysis.
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Peltier, R., Brimble, M.A., Wojnar, J.M., Williams, D.E., Evans, C.W. and DeVries, A.L. 2010 Synthesis and antifreeze activity of fish antifreeze glycoproteins and their analogues. Chemical Science 1 538-551 10.1039/c0sc00194e
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20091206
Ending_Date: 20100113
Currentness_Reference:Unknown
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20101025
Ending_Date: 20101203
Currentness_Reference:Unknown
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20111112
Ending_Date: 20111223
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 166.77
East_Bounding_Coordinate: 166.311
North_Bounding_Coordinate: -77.635
South_Bounding_Coordinate: -77.885
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT
Theme_Keyword: LABORATORY
Theme_Keyword: FIELD INVESTIGATION
Theme_Keyword: BIOTA
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: CONTINENT > ANTARCTICA > MCMURDO SOUND
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CLIVE EVANS
Contact_Position: INVESTIGATOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: School of Biological Sciences
Address: Molecular, Cellular and Developmental Biology Section
Address: University of Auckland
Address: Private Bag 92019
City: Auckland
Postal_Code: 1142
Country: New Zealand
Contact_Voice_Telephone: +64 9 373 7599 xtn 87245
Contact_Facsimile_Telephone: +64 9 373 7415
Contact_Electronic_Mail_Address: c.evans@auckland.ac.nz
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
All samples are held by the investigator at the School of Biological Sciences, University of Auckland, New Zealand. Please contact the investigator for more information.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Resource_Description: K014_2009_2010_NZ_1
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.antarcticanz.govt.nz/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20081017
Metadata_Review_Date: 20120404
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CEISHA POIROT
Contact_Person_Primary:
Contact_Person: SHULAMIT GORDON
Contact_Position: DIF AUTHOR
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: c.poirot@antarcticanz.govt.nz
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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