Soil samples and temperature data from Tramway Ridge, Mt Erebus to determine the genetic capabilities and structure of microbial communities

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: Soil samples and temperature data from Tramway Ridge, Mt Erebus to determine the genetic capabilities and structure of microbial communities
Description:
Abstract:
The study of high temperature extreme environments continues to challenge our understanding of the upper tolerances of microbial life and how life may have originated on earth and possibly other planets. The Tramway Ridge geothermal site on Mt. Erebus, an active volcano in Antarctica, is the most geographically isolated geothermal site on earth providing an excellent system for studies of microbial speciation, biogeography, and evolution of thermal adaptation. Recent advances in high throughput DNA sequencing and bioinformatics allow us to acquire and decipher the genetic capabilities and structure of entire microbial communities without the necessity of cultivation. Employing a combination of these advanced genetic methods coupled with culture dependant approaches a gene-centric analysis of the Tramway Ridge microflora and other Antarctic geothermal sites was undertaken to address questions focused on endemism, biogeography, evolution, and adaptation. Soil samples and bacterial isolates were collected from high temperature soils (maximum temperature sites) at Tramway Ridge and western crater locations at Mt Erebus. These temperatures averaged 65°C and were dominated by steam emissions. Samples have been obtained both for DNA/RNA genetic analysis and for cultivation. Cultivation efforts were undertaken on site and have been continued successfully in the lab. Ice core samples were also collected from walls of several ice chimneys. Temperature probes were installed in the high temperature soils at Tramway Ridge. The temperature probes and data loggers were left in situ to obtain temperature data over a one year period at each site. Temperature, Depth, Water Activity and Oxygen saturation was measured in field. Salinity, pH, moisture content, nutrients and elemental analysis were determined post-field.
Purpose:
Not Available
Supplemental_Information:
Not Available
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20090123
Ending_Date: 20090123
Currentness_Reference:Unknown
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20091121
Ending_Date: 20091126
Currentness_Reference:Unknown
Status:
Progress: In Work
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 167.1114
East_Bounding_Coordinate: 167.1114
North_Bounding_Coordinate: -77.5517
South_Bounding_Coordinate: -77.5517
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 167.1108
East_Bounding_Coordinate: 167.1527
North_Bounding_Coordinate: -77.5183
South_Bounding_Coordinate: -77.5294
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: GCMD INSTRUMENT
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > SPECIES/POPULATION INTERACTIONS > EVOLUTIONARY ADAPTATION
Theme_Keyword: FIELD SURVEYS
Theme_Keyword: LABORATORY
Theme_Keyword: SOIL SAMPLER
Theme_Keyword: SOIL TEMPERATURE PROBE
Theme_Keyword: BIOTA
Theme_Keyword: ELEVATION
Theme_Keyword: ENVIRONMENT
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA > MOUNT EREBUS
Place_Keyword: CONTINENT > ANTARCTICA > TRAMWAY RIDGE
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CRAIG CARY
Contact_Position: INVESTIGATOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Biological Sciences
Address: University of Waikato
Address: PO BOX 3105
City: Hamilton
Postal_Code: 3105
Country: New Zealand
Contact_Voice_Telephone: +64 7 838 4593
Contact_Facsimile_Telephone: +64 7 838 4218
Contact_Electronic_Mail_Address: caryc@UDel.Edu
Contact_Electronic_Mail_Address: c.cary@waikato.ac.nz
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
All of the collected samples are stored at the University of Waikato, Hamilton New Zealand in a PC2 containment facility. Various amounts of the samples are held at the University of Western Cape, Cape Town, South Africa. The samples are stored at -80C. The samples are being used for DNA analysis and this analysis uses very little material. Therefore, most if not all, of the samples still exist. Please contact the investigator for more information.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Resource_Description: K023_2008_2009_NZ_1
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.antarcticanz.govt.nz/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20090430
Metadata_Review_Date: 20100920
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CEISHA POIROT
Contact_Person_Primary:
Contact_Person: SHULAMIT GORDON
Contact_Position: DIF AUTHOR
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: c.poirot@antarcticanz.govt.nz
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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