The identification, function and cold adaptation of the proteins/enzymes in Antarctic fish from amount and distribution of lactate dehydrogenase from seven different tissues

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Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: The identification, function and cold adaptation of the proteins/enzymes in Antarctic fish from amount and distribution of lactate dehydrogenase from seven different tissues
Description:
Abstract:
The cold adaptation in the proteins/enzymes of Antarctic fish was investigated in collaboration with the American Antarctic program. The likely function of lactate dehydrogenase (LDH), the only isozyme found in these fish, was examined by looking at the amount and distribution of LDH in a variety of both temperate and Antarctic fish. Small samples were collected from 7 different tissues (eye, brain, heart, liver, spleen and red and white muscles) of 4-8 individuals of each species of fish (Pagothenia borchgrevinki, Trematomus nicolai, T. newnesi and T. pennelli). Each sample was assayed by creating an extract from a tissue chunk, removing the debris by centrifugation and the produced supernatant assayed for enzyme activity spectrophotometrically. To determine the nature of the new isozyme found in these fish, tissue was collected and stored. A protocol was developed to stabilize the isozyme, to be able to purify it and then determine the amino acid sequence of the amino terminus, in order to identify the new type of isozyme. Lactate dehydrogenase, the enzyme, and another enzyme, citrate synthase, are important in the metabolism of these fish. A survey of the enzyme activity of these enzymes in a number of species of fish was completed. Samples were collected from 7 different tissues from 8 individuals of P. borchgrevinki, T. nicolai, T. newnesi and T. pennelli and analysed for enzyme activity.
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Marshall, C. Cold-adapted enzymes. Trends in biotechnology 15(9): 359-364, 1997. Marshall, C. Crossman, D. Love, C. McInnes, S. Flemming, R. Structural studies of cold-adaptation: lactate dehydrogenases from notothenioid fish. in: Antarctic Ecosystems: models for wider ecological understanding. Davison W. Howard-Williams C. Broady P.(eds) Christchurch, New Zealand: New Zealand Natural Sciences. 2000. pp.123-133 Marshall, C.J. Johnston, N.M. Murray, B.W. Brown, P.M. Verghese, A.I. Does the enzyme citrate synthase from several Antarctic fish show evidence of cold adaptation? in: Antarctic Biology in a Global Context. Huiskes A.H.L. Gieskes W.W.C. Rozema J. Leiden, The Netherlands: Backhuys Publishers. 2003. pp.102-106 Sharpe, M. Love, C. Marshall, C. Lactate dehydrogenase from the Antarctic eelpout, Lycodichthys dearborni. Polar biology 24(4): 258-269, 2001.
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 19951010
Ending_Date: 20011215
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 166.75
East_Bounding_Coordinate: 166.75
North_Bounding_Coordinate: -77.85
South_Bounding_Coordinate: -77.85
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: PROJECT
Theme_Keyword_Thesaurus: ANCILLARY KEYWORDS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH > RAY-FINNED FISHES > PERCH-LIKE FISHES
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH
Theme_Keyword: EBA > EVOLUTION AND BIODIVERSITY IN THE ANTARCTIC
Theme_Keyword: Lactate Dehydrogenase
Theme_Keyword: Enzymes
Theme_Keyword: BIOTA
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: GEOGRAPHIC REGION > POLAR
Place_Keyword: OCEAN > SOUTHERN OCEAN > ROSS SEA
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CRAIG MARSHALL
Contact_Position: INVESTIGATOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Biochemistry
Address: University of Otago
Address: PO Box 56
Address: Dunedin
Country: NEW ZEALAND
Contact_Voice_Telephone: +64 3 479 7570
Contact_Facsimile_Telephone: +64 3 4797866
Contact_Electronic_Mail_Address: craig.marshall@stonebow.otago.ac.nz
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Any existing tissue samples are held at the University of Otago. Please contact the investigator for more information and access to these samples.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Resource_Description: K066_2000_2003_NZ_2
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.antarcticanz.govt.nz/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20040602
Metadata_Review_Date: 20090327
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CEISHA POIROT
Contact_Person_Primary:
Contact_Person: SHULAMIT GORDON
Contact_Position: DIF AUTHOR
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: c.poirot@antarcticanz.govt.nz
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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