The activity and expression of DNA repair/photoreactivation with the enzyme photolyase in the sea urchin Sterechinus neumayeri, McMurdo Sound

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Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: The activity and expression of DNA repair/photoreactivation with the enzyme photolyase in the sea urchin Sterechinus neumayeri, McMurdo Sound
Description:
Abstract:
An important aspect of understanding the impact of increased UVR is assessing the ability of Antarctic species to repair DNA damaged through the process of photoreactiviation or photorepair. To repair DNA damage organisms have evolved the enzyme photolyase, a light dependent enzyme that requires UVR and visible irradiance to be catalytic. To quantify the importance of photorepair rates in Antarctic invertebrate larvae and the importance in mitigating effects of UVR four experiments were conducted. 1. Photorepair was examined in embryos and larvae of the sea urchin Sterechinus neumayeri. Photoreactivation at the whole organism level (eggs) and at the molecular level in terms of the production and disappearance of cyclobutance pyrimidine dimers (CPDs) in DNA in eggs, blastula and 4 armed larvae was analysed. 2. Freshly fertilised S. neumayeri eggs were exposed to either 0 (control), 1 or 2 hours of UVR and the time to when 50% of the eggs have undergone first cell division recorded. Cleavage delay as a result of UV exposure can then be expressed as a percentage. Following this, a further experiment was carried out where UVR induced cleavage delay was measured in eggs where photorepair was stimulated (exposed to visible light) or inhibited (kept in the dark). 3. The production of CPDs over time was examined in the eggs, blastula and 4 armed larvae of S. neumayeri which were exposed to UVR for a total of 5 hours. Samples were taken at time 0 and at 1 hourly intervals. Embryos were fixed in buffered DMSO and later the DNA extracted and CPDs quantified. 4. To obtain a measure of the rate of photoreactivation, the disappearance of CPDs over time in S. neumayeri embryos (fertilised eggs, blastula and 4 armed larvae) that were either able to photorepair or were photorepair inhibited was examined. Embryos were exposed to UVB then allowed to photorepair by placement under a full spectrum light or total darkness (photorepair inhibited). Samples were taken at 0, 1, 2, 6, 12 and 24 hours post UV exposure. Embryos were fixed in buffered DMSO and later the DNA extracted and CPDs quantified. Identical experiments were carried out on temperate New Zealand echinoderm species and other Antarctic species to obtain a comparative rate of photoreactivation; Perknaster, Parbolarsia and Acodontaster (all freshly fertilized larvae).
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Isely, N., Lamare, M.D., Marshall, C., Barker, M. 2009. Expression of the DNA repair enzyme, photolyase, in developmental tissues and larvae, and in response to ambient UV-R in the Antarctic sea urchin Sterechinus neumayeri. Photochemistry and photobiology 85: 1168–1176 2009. doi: 10.1111 / j.1751-1097.2009.00566.x
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20031027
Ending_Date: 20031116
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 164.4194
East_Bounding_Coordinate: 166.7595
North_Bounding_Coordinate: -77.0622
South_Bounding_Coordinate: -77.8608
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC RADIATION > ULTRAVIOLET RADIATION
Theme_Keyword: EARTH SCIENCE > OCEANS > OCEAN OPTICS > ATTENUATION/TRANSMISSION
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ECHINODERMS > SEA URCHINS
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > ZOOPLANKTON
Theme_Keyword: BIOTA
Theme_Keyword: ENVIRONMENT
Theme_Keyword: OCEANS
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA > MCMURDO SOUND
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: MILES LAMARE
Contact_Position: INVESTIGATOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Marine Science
Address: University of Otago
Address: PO Box 56
City: Dunedin
Country: New Zealand
Contact_Voice_Telephone: +64 3 479 7463
Contact_Facsimile_Telephone: +64 3 479 8336
Contact_Electronic_Mail_Address: miles.lamare@stonebow.otago.ac.nz
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Any collected samples get destroyed in their analysis. Field notebooks are held by the investigator, please contact for more information.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Resource_Description: K068_2003_2004_NZ_1
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.antarcticanz.govt.nz/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.otago.ac.nz/marinesciencehttp://www.ota...
Access_Instructions:
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20051101
Metadata_Review_Date: 20100223
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: SHULAMIT GORDON
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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