The ability to photorepair UV damaged DNA and the rate of photoreactivation in Antarctic species compared with temperate and tropical species

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: The ability to photorepair UV damaged DNA and the rate of photoreactivation in Antarctic species compared with temperate and tropical species
Description:
Abstract:
Time course experimental exposure of the Antarctic sea urchin Sterechinus neumayeriembryos to UVR was carried out in a lab to examine the kinetics of UVR induced damage to DNA. Embryos (freshly fertilized embryos, blastula and 4 armed larvae) were exposed to artificial light with samples taken at 0, 1, 2, 6, 12 and 24 hours post exposure. Cyclobutane pyrimidine dimers (CPDs) were quantified. To obtain a measure of the rate of photoreactivation, the disappearance of CPDs over time in embryos that were either able to photorepair or were photorepair inhibited in two Antarctic species (Sterechinus neumayeri and Odontaster meridionalis) a temperate species (Pseudechinus huttoni) and two tropical species (Arachnoides spp. and Diadema spp.) was determined. The embryos were exposed to UVB for 1 hour then allowed to photorepair by placement under a full spectrum light or total darkness. Samples were taken at 0, 1, 2, 6, 12 and 24 hours post UV exposure. DNA photoproducts were detected by an enzyme-linked immunoabsorbent assay. The effect of temperature on DNA repair rate was quantified by running the above experiment at various temperatures depending on species. For Antarctic species, experiments were conducted at -1.9, 0 and 2.0 °C, for temperate species at 5, 10 and 15°C and tropical species at 23.5, 28 and 32°C. Time to repair 50% and 80% of the damage and percent repaired after 24 hours was calculated for each Antarctic species and compared with the temperate and tropical species.
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Lamare, M.D., Barker, M.F. And Lesser, M.P. 2007. In situ rates of DNA damage and abnormal development in Antarctic and non-Antarctic sea urchin embryos. Aquatic Biology 1: 21-32.
Supplemental_Information:
REFERENCE: Lamare, M.D., Barker, M.F., Lesser, M.P. And Marshall, C.J. 2006. DNA photorepair in echinoid embryos: effects of temperature on repair rate in Antarctic and non-Antarctic species. J. Exp. Biol. 209:5017-5028.
Supplemental_Information:
REFERENCE: Lesser, M.P., Baryy, T.M., Lamare, M.D. And Barker, M.F. 2006. Biological weighting functions for DNA damage in sea urchin embryos exposed to ultraviolet radiation. J. Exp. Mar. Biol. Ecol. 328: 10-21
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20021001
Ending_Date: 20041230
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 164.4194
East_Bounding_Coordinate: 166.7595
North_Bounding_Coordinate: -77.0622
South_Bounding_Coordinate: -77.8608
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC RADIATION > ULTRAVIOLET RADIATION
Theme_Keyword: EARTH SCIENCE > OCEANS > OCEAN OPTICS > ATTENUATION/TRANSMISSION
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ECHINODERMS > SEA URCHINS
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > ZOOPLANKTON
Theme_Keyword: BIOTA
Theme_Keyword: ENVIRONMENT
Theme_Keyword: OCEANS
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: CONTINENT > ANTARCTICA > MCMURDO SOUND
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: MILES LAMARE
Contact_Position: INVESTIGATOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Marine Science
Address: University of Otago
Address: PO Box 56
City: Dunedin
Country: New Zealand
Contact_Voice_Telephone: +64 3 479 7463
Contact_Facsimile_Telephone: +64 3 479 8336
Contact_Electronic_Mail_Address: miles.lamare@stonebow.otago.ac.nz
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
The data is contained in the publications. Any collected samples get destroyed in their analysis. Field notebooks are held by the investigator, please contact for more information.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Resource_Description: K068_2004_2006_NZ_2
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.antarcticanz.govt.nz/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.otago.ac.nz/marinesciencehttp://www.ota...
Access_Instructions:
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20051101
Metadata_Review_Date: 20090327
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: SHULAMIT GORDON
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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