Diversity and metabolic abilities of Antarctic bacteria - DGGE fingerprinting of bacteria

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: Diversity and metabolic abilities of Antarctic bacteria - DGGE fingerprinting of bacteria
Description:
Abstract:
Bacterial community structures in soils collected from eight sites around Casey Station, Antarctica, were investigated using denaturing gradient gel electrophoresis (DGGE) of amplified 16S rRNA gene fragments. Higher bacterial diversity was found in soils from protected or relatively low human-impacted sites in comparison to highly impacted sites. However, the highest diversity was detected in samples from Wilkes Tip, a former waste disposal site that has been undisturbed for the last 50 years. Comparison of community structure based on non-metric multidimensional scaling plots revealed that all sites, except the hydrocarbon-contaminated (oil spill) site, were clustered with a 45% similarity. A total of 23 partial 16S rRNA gene sequences were obtained from the excised DGGE bands, with the majority of the sequences closely related to those of the Cytophaga-Flexibacter-Bacteroides group. No significant correlation was established between environmental variables, including soil pH, electrical conductivity, carbon, nitrogen, water content and heavy metals, with bacterial diversity across the eight study sites. Irene K. P. Tan - Project leader C. W. Chong - sample collection, molecular and statistical analyses G. Y. Annie Tan - provided some advice in molecular analysis Richard C. S. Wong - provided advice in chemical analyses Martin J. Riddle - assisted in manuscript writing and provided advice in metal analyses Locations used in this experiment were: - Casey Red Shed - Oil Spill site - Thala Valley - Wilkes Tip - Browning Peninsula - Mitchell Peninsula - ASPA 135 - ASPA 136
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Chong C. W., Tan G. Y., Wong R. C. S. Riddle M. J., Tan I. K. P 2009 DGGE fingerprinting of bacteria in soils from eight ecologically different sites around Casey Station, Antarctica Polar Biology 32 853-860 DOI:10.1007/s00300-009-0585-6
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20051211
Ending_Date: 20051218
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
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East_Bounding_Coordinate: 110.5225
North_Bounding_Coordinate: -66.2827
South_Bounding_Coordinate: -66.2827
Spatial_Domain:
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East_Bounding_Coordinate: 110.5252
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Spatial_Domain:
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East_Bounding_Coordinate: 110.5372
North_Bounding_Coordinate: -66.2802
South_Bounding_Coordinate: -66.2802
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East_Bounding_Coordinate: 110.5394
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South_Bounding_Coordinate: -66.2597
Spatial_Domain:
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East_Bounding_Coordinate: 110.5497
North_Bounding_Coordinate: -66.4722
South_Bounding_Coordinate: -66.4722
Spatial_Domain:
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East_Bounding_Coordinate: 110.5486
North_Bounding_Coordinate: -66.3141
South_Bounding_Coordinate: -66.3141
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 110.5388
East_Bounding_Coordinate: 110.5388
North_Bounding_Coordinate: -66.2819
South_Bounding_Coordinate: -66.2819
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 110.5375
East_Bounding_Coordinate: 110.5375
North_Bounding_Coordinate: -66.2522
South_Bounding_Coordinate: -66.2522
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: ANCILLARY KEYWORDS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
Theme_Keyword: EARTH SCIENCE > LAND SURFACE > SOILS > MICROFAUNA
Theme_Keyword: FIELD INVESTIGATION
Theme_Keyword: FIELD SURVEYS
Theme_Keyword: LABORATORY
Theme_Keyword: Bacteria
Theme_Keyword: Denaturing Gradient Gel Electrophoresis
Theme_Keyword: Dgge
Theme_Keyword: BIOTA
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA > WINDMILL ISLANDS
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: There are no data from this part of the project - see the quality field for further information.
Use_Constraints:
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2341_DGGE when using these data.
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: IRENE KIT-PING TAN
Contact_Person_Primary:
Contact_Person: CHUN WIE CHONG
Contact_Person_Primary:
Contact_Person: ANNIE GEOK YUAN TAN
Contact_Person_Primary:
Contact_Person: RICHARD CS WONG
Contact_Person_Primary:
Contact_Person: MARTIN J. RIDDLE
Contact_Position: INVESTIGATOR
Contact_Position: TECHNICAL CONTACT
Contact_Position: TECHNICAL CONTACT
Contact_Position: TECHNICAL CONTACT
Contact_Position: TECHNICAL CONTACT
Contact_Position: TECHNICAL CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: University of Malaya
Address: FACULTY OF SCIENCE
Address: Institute of Biological Sciences
City: Kuala Lumpur
Postal_Code: 50603
Country: Malaysia
Contact_Address:
Address_Type: Mailing and Physical Address
Address: International Medical University
Address: No. 126, Jalan Jalil Perkasa 19, Bukit Jalil
City: Kuala Lumpur
Postal_Code: 57000
Country: Malaysia
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Institute of Biological Sciences
Address: University of Malaya
City: Kuala Lumpur
Postal_Code: 50603
Country: Malaysia
Contact_Address:
Address_Type: Mailing and Physical Address
Address: University of Malaya
Address: FACULTY OF SCIENCE
Address: Department of Chemistry
City: Kuala Lumpur
Postal_Code: 50603
Country: Malaysia
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +60 3 7967 6792
Contact_Voice_Telephone: 60379674634
Contact_Voice_Telephone: +61 3 6232 3573
Contact_Facsimile_Telephone: +60 3 7967 4178
Contact_Facsimile_Telephone: 60379605935
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: itan@um.edu.my
Contact_Electronic_Mail_Address: cchhoonngg81@yahoo.com
Contact_Electronic_Mail_Address: gytan@um.edu.my
Contact_Electronic_Mail_Address: Richard@um.edu.my
Contact_Electronic_Mail_Address: martin.riddle@aad.gov.au
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
The DGGE studies were to compare bacterial diversity between sites, based on band patterns. The 16S DNA fragments used were very short, about 500 bp only. The sequence of individual bands were not sufficient to identify the bacteria. Hence, there are no accession numbers.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: AU/AADC > Australian Antarctic Data Centre, Australia
Contact_Person: DATA OFFICER AADC
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +61 3 6232 3244
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: metadata@aad.gov.au
Resource_Description: ASAC_2341_DGGE
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
https://secure3.aad.gov.au/proms/public/projects/r...
Access_Instructions:
Public information for AAS project 2341
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/metadata/citation.cfm?...
Access_Instructions:
Citation reference for this metadata record and dataset
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20121112
Metadata_Review_Date: 20121220
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: DAVE J. CONNELL
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +61 3 6232 3244
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: dave.connell@aad.gov.au
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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