Molecular analysis of microbiota trapped in ancient antarctic glacial ice

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: Molecular analysis of microbiota trapped in ancient antarctic glacial ice
Description:
Abstract:
Metadata record for data from ASAC Project 2763 See the link below for public details on this project. Ancient Antarctic glacial ice is a potential resource of trapped microorganisms dating back several hundreds of thousand years that give a snapshot of the past. Nucleic acid, such as DNA, has been identified in samples as old as these from Bacteria, Archaea and Viruses, and this will be the focus of this study. Outcomes of this research will determine the type of organisms that become trapped in these ancient samples, and whether they are able to survive such an extreme condition, and may even lead to novel species being discovered, or even new genes and products. Project objectives: Determine the type of microorganisms that become trapped in ancient Antarctic glacial ice and ascertain whether glacial ice in Antarctica harbours biota that are of evolutionary or biotechnological interest. Public summary of the season progress: Ancient glacial ice samples were collected from ice cliff areas located near Casey Station during the just recent Summer expedition. Ice samples were transported back to Australia and will be subject to 454 sequencing analysis in the next few months in collaboration with Prof. Alan Cooper, Centre for Ancient DNA, University of Adelaide. Other work being performed was completed included an initial molecular-based microbial survey of unusual alkaline, permanently ice-covered, continental lakes located in the Framnes Mountains, inland from Mawson The first download file contains: 10 files File 1. Chemical and oxygen isotope data for Law Dome ice cores that were used in microbiological studies (isolation and DNA analyses). File 2. Chemical and oxygen isotope data for an Amery Ice Shelf ice core that was used in microbiological studies (isolation and DNA analyses). File 3. 16S rRNA gene sequence data obtained from Law Dome ice core samples. All sequences are clones derived after direct PCR amplification of DNA extracts, no isolates were obtained. File 4. 16S rRNA gene sequence data obtained from Amery Ice Shelf ice core sample. All sequences are clones derived after direct PCR amplification of DNA extracts, no isolates were definitively obtained. File 5. Soil and similar samples obtained from either the Vestfold Hills, Eastern Antarctica or from Macquarie Harbour. These soils are the source of actinobacteria screened in the project for antimicrobial activity. File 6. Actinobacteria/actinomycete isolates information detailing isolation procedure, colonial/cellular characteristics, and tentative identification. Barcode system was used to track isolates. Represents the "(University of Tasmania Antarctic Actinobacteria) UTAA" collection. File 7. Preliminary antimicrobial screening trial data for 1267 Antarctic actinobacterial isolates against 5 strains of Listeria monocytogenes. A secondary screen was performed to identify those with reliable bioactivity. File 8. Broader analysis of antimicrobial activities of Antarctic isolates against a panel of bacterial pathogenic bacteria. All strains were identified to species level within the genus Streptomyces. File 9. Hydrocarbon profiles of selected Antarctic actinobacterial strains against a range of aliphatic and polycyclic hydrocarbons. File 10. Phenotypic, chemotaxonomic and identification (by 16S rRNA gene sequencing) data of Antarctic hydrocarbon degrading strains. The second download file contains: 3 files: File 1. Framnes Mountain epiglacial lake sample data. Indicates lakes sampled, location, chemical data (pH, temperature). File 2. Cloned 16S rRNA gene sequences obtained from Patterned Lake water DNA extracts. All sequnces are in FASTA format. File 3. Cloned 16S rRNA gene sequences obtained from Sonic Lake water DNA extracts. All sequnces are in FASTA format.
Purpose:
Not Available
Supplemental_Information:
Not Available
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20061001
Ending_Date: 20070331
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 110.0
East_Bounding_Coordinate: 111.0
North_Bounding_Coordinate: -66.0
South_Bounding_Coordinate: -67.0
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: ANCILLARY KEYWORDS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > CRYOSPHERE > GLACIERS/ICE SHEETS > GLACIERS
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
Theme_Keyword: FIELD SURVEYS
Theme_Keyword: Glaciers
Theme_Keyword: Bacteria
Theme_Keyword: Viruses
Theme_Keyword: Dna
Theme_Keyword: BIOTA
Theme_Keyword: CLIMATOLOGY/METEOROLOGY/ATMOSPHERE
Theme_Keyword: ENVIRONMENT
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA > CASEY
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: The data are publicly available for download from the provided URLs.
Use_Constraints:
This data set conforms to the PICCCBY Attribution License (http://creativecommons.org/licenses/by/3.0/). Please follow instructions listed in the citation reference provided at http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=ASAC_2763 when using these data.
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: JOHN BOWMAN
Contact_Position: INVESTIGATOR
Contact_Position: TECHNICAL CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: School of Agricultural Science
Address: University of Tasmania
Address: Private Bag 54
City: Hobart
State_or_Province: Tasmania
Postal_Code: 7001
Country: Australia
Contact_Voice_Telephone: +61 3 6226 6380
Contact_Facsimile_Telephone: +61 3 6226 2642
Contact_Electronic_Mail_Address: john.bowman@utas.edu.au
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
The Dates provided in temporal coverage are approximate only, and represent the beginning and end of the 2006 - 2007 Antarctic seasons. The latitudes and longitudes provided in spatial coverage are approximate only.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: AU/AADC > Australian Antarctic Data Centre, Australia
Contact_Person: DATA OFFICER AADC
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +61 3 6232 3244
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: metadata@aad.gov.au
Resource_Description: ASAC_2763
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: csv, text
Format_Name: csv, text
Transfer_Size: 56 kb
Transfer_Size: 7 kb
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/portal/download_file.c...
Access_Instructions:
Download point for the data - part 1
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/portal/download_file.c...
Access_Instructions:
Download point for the data - part 2
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
https://secure3.aad.gov.au/proms/public/projects/r...
Access_Instructions:
Public information for ASAC project 2763
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://data.aad.gov.au/aadc/metadata/citation.cfm?...
Access_Instructions:
Citation reference for this metadata record and dataset
Fees: Free
Fees: Free
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Metadata_Reference_Information:
Metadata_Date: 20060727
Metadata_Review_Date: 20140110
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: DAVE J. CONNELL
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Australian Antarctic Division
Address: 203 Channel Highway
City: Kingston
State_or_Province: Tasmania
Postal_Code: 7050
Country: Australia
Contact_Voice_Telephone: +61 3 6232 3244
Contact_Facsimile_Telephone: +61 3 6232 3351
Contact_Electronic_Mail_Address: dave.connell@aad.gov.au
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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