GCMD

Investigation of the role of anti-freeze glyco-proteins in the circulation system of Antarctic fish

Federal Geographic Data Committee (FGDC) Metadata:

Identification_Information
Data_Quality_Information
Spatial_Reference_Information
Distribution_Information
Metadata_Reference_Information

Identification_Information:

Citation:

Citation_Information:

Originator: Unknown
Publication_Date: Unknown
Title: Investigation of the role of anti-freeze glyco-proteins in the circulation system of Antarctic fish

Description:

Abstract:: In order to investigate how Antarctic fish deal with ice in their blood circulatory system, Trematomus bernacchii and Pagothenia borchgrevinki Antarctic fish were captured for experimental investigation. A variety of biochemical techniques were used to isolate Anti-Freeze Glyco-Proteins (AFGPs) from Antarctic fish blood, which was then conjugated directly to a fluorescent marker. We synthesised silicon dioxide nanobeads incorporating a fluorescent probe and coupled these to AFGP via a multimeric linking protein or dendrimer based on G4.5 poly(amidoamine). These modified nanobeads, consisting of a fluorescent particle surrounded by AFGPs, were designed to serve as a proxy for ice crystals that have bound AFGPs. When introduced into the circulation of the fish, we were able to follow the fate of free fluorescent AFGP and particle-bound fluorescent AFGP (the latter serving as a proxy for ice in the circulation). Frozen and alcohol-fixed tissues were returned to New Zealand for further laboratory analysis.
Purpose:: Not Available
Supplemental_Information:: REFERENCE: Peltier, R., Brimble, M.A., Wojnar, J.M., Williams, D.E., Evans, C.W. and DeVries, A.L. 2010 Synthesis and antifreeze activity of fish antifreeze glycoproteins and their analogues. Chemical Science 1 538-551 10.1039/c0sc00194e

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 20091206
Ending_Date: 20100113

Currentness_Reference:Unknown

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 20101025
Ending_Date: 20101203

Currentness_Reference:Unknown

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 20111112
Ending_Date: 20111223

Currentness_Reference:Unknown

Status:

Progress: Complete
Maintenance_and_Update_Frequency: As needed

Spatial_Domain:

Description_of_Geographic_Extent:

Bounding_Coordinates:

West_Bounding_Coordinate: 166.77
East_Bounding_Coordinate: 166.311
North_Bounding_Coordinate: -77.635
South_Bounding_Coordinate: -77.885

Keywords:

Theme:

Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/VERTEBRATES > FISH
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT
Theme_Keyword: LABORATORY
Theme_Keyword: FIELD INVESTIGATION
Theme_Keyword: BIOTA
Theme_Keyword: ENGLISH

Place:

Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: CONTINENT > ANTARCTICA > MCMURDO SOUND
Place_Keyword: GEOGRAPHIC REGION > POLAR

Access_Constraints: Not Available

Use_Constraints:

Not Available

Point_of_Contact:

Contact_Information:

Contact_Person_Primary:

Contact_Person: CLIVE EVANS

Contact_Position: INVESTIGATOR

Contact_Address:

Address_Type: Mailing and Physical Address
Address: School of Biological Sciences
Address: Molecular, Cellular and Developmental Biology Section
Address: University of Auckland
Address: Private Bag 92019
City: Auckland
Postal_Code: 1142
Country: New Zealand

Contact_Voice_Telephone: +64 9 373 7599 xtn 87245

Contact_Facsimile_Telephone: +64 9 373 7415

Contact_Electronic_Mail_Address: c.evans@auckland.ac.nz

Data_Quality_Information:

Attribute_Accuracy:

Attribute_Accuracy_Report:: All samples are held by the investigator at the School of Biological Sciences, University of Auckland, New Zealand. Please contact the investigator for more information.

Logical_Consistency_Report:

Not Available

Completeness_Report:

Not Available

Lineage:

Process_Step:

Process_Description:: Not Available
Process_Date: Unknown

Spatial_Reference_Information:

Distribution_Information:

Distributor:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON

Contact_Position: DATA CENTER CONTACT

Contact_Address:

Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand

Contact_Voice_Telephone: +64 3 358 0200

Contact_Facsimile_Telephone: +64 3 358 0211

Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz

Resource_Description: K014_2009_2010_NZ_1

Distribution_Liability:

Not Available

Standard_Order_Process:

Digital_Form:

Digital_Transfer_Information:

Format_Name: Not Available

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:: http://www.antarcticanz.govt.nz/

Access_Instructions:

DATA CENTER URL

Digital_Transfer_Option:

Fees: Not Available

Metadata_Reference_Information:

Metadata_Date: 20081017

Metadata_Review_Date: 20120404

Metadata_Contact:

Contact_Information:

Contact_Person_Primary:

Contact_Person: CEISHA POIROT

Contact_Person_Primary:

Contact_Person: SHULAMIT GORDON

Contact_Position: DIF AUTHOR

Contact_Address:

Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand

Contact_Address:

Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand

Contact_Voice_Telephone: +64 3 358 0200

Contact_Facsimile_Telephone: +64 3 358 0211

Contact_Electronic_Mail_Address: c.poirot@antarcticanz.govt.nz

Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz

Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata

Metadata_Standard_Version: FGDC-STD-001-1998

Metadata_Time_Convention: local time