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Estudio de los efectos de la radiacion UV incidente sobre cepas aisladas y comunidades bacterianas marinas antarticas.

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: Estudio de los efectos de la radiacion UV incidente sobre cepas aisladas y comunidades bacterianas marinas antarticas.
Edition:
Geospatial_Data_Presentation_Form:
Series_Information:
Series_Name:
Issue_Identification:
Publication_Information:
Publication_Place:
Publisher:
Other_Citation_Details:
PICTA-10-2004
Online_Linkage:
Description:
Abstract:
ENGLISH Since the crucial role having by the microorganisms in the nutrient cycling of the coastal marine ecosystem in Antarctica, the aim of this project is to analyze the effect of the incident UV radiation (enhanced by the stratospheric ozone layer) on the Antarctic marine bacterial flora. Response of isolated strains and natural communities obtained from different water column levels will be analyzed in Potter Cove, Antarctica. In this instance of the study we will use interferential filters in order to fractionate the previously studied UV-A and UV-B fractions in more narrows bands. In addition, the role of the water column as a natural factor of solar radiation attenuation will be analyzed using fixed and dynamics systems that permit us a better simulation of the vertical mixing regime. Bacterial viability, DNA damage and induction of oxidative stress-related enzymes will be evaluated. Field experiments will be complemented and compared with laboratory irradiation assays under controlled conditions. Due to that the standards methods of bacterial taxonomy are inadequate for environmental strains, we will use 16S rDNA sequencing to classify the isolates. Changes in the structure of bacterial communities will be inferred using RFLP techniques and, if it is possible, by DGGE and SSCP SPANISH Dado el papel fundamental que los microorganismos cumplen en el ciclo de los nutrientes del ecosistema marino costero antartico, este proyecto tiene por objetivo analizar el efecto que la radiacion UV incidente en Antartida (incrementada por el agujero de ozono estratosferico) tiene sobre la flora bacteriana marina. Se analizara la respuesta de cepas aisladas y comunidades naturales de distintas profundidades a diferentes regimenes de radiacion UV, en Caleta Potter, Antartida. En esta etapa se utilizaran filtros interferenciales que permitan desdoblar, las ya estudiadas fracciones UV-A y UV-B en bandas menores. Adicionalmente se estudiara el efecto de la columna de agua como filtro natural de la radiacion, utilizando sistemas estaticos y sistemas dinamicos que reflejen mejor el regimen de mezcla vertical natural. Se evaluara la viabilidad, el dano al ADN y la induccion de enzimas de estres oxidativo. Los ensayos a campo se complementaran y compararan con ensayos de irradiacion en laboratorio. Dado que los metodos convencionales de clasificacion bacteriana son poco satisfactorios con cepas ambientales, se utilizara la secuenciacion de ADNr 16S para tal fin. Ademas, los cambios en la estructura de las comunidades bacterianas se inferiran mediante tecnicas de RFLP y, en lo posible mediante DGGE o SSCP.
Purpose:
Not Available
Supplemental_Information:
Not Available
Status:
Progress: In Work
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: -180.0
East_Bounding_Coordinate: 180.0
North_Bounding_Coordinate: -62.83
South_Bounding_Coordinate: -90.0
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: PROJECT
Theme_Keyword_Thesaurus: ANCILLARY KEYWORDS
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC RADIATION > SOLAR RADIATION
Theme_Keyword: EARTH SCIENCE > ATMOSPHERE > ATMOSPHERIC RADIATION > ULTRAVIOLET RADIATION
Theme_Keyword: EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > NUTRIENTS
Theme_Keyword: EARTH SCIENCE > OCEANS > OCEAN OPTICS > PHOTOSYNTHETICALLY ACTIVE RADIATION
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > COMMUNITY STRUCTURE
Theme_Keyword: PICTA > PROJECT FOR ANTARCTIC SCIENCE AND TECHNOLOGY
Theme_Keyword: Microbial Ecology
Theme_Keyword: Uv Radiation
Theme_Keyword: Antarctic Bacterial Communities
Theme_Keyword: Molecular Taxonomy
Theme_Keyword: Psycrophilic Bacteria
Theme_Keyword: BIOTA
Theme_Keyword: CLIMATOLOGY/METEOROLOGY/ATMOSPHERE
Theme_Keyword: ENVIRONMENT
Theme_Keyword: GEOSCIENTIFIC INFORMATION
Theme_Keyword: INLAND WATERS
Theme_Keyword: OCEANS
Theme_Keyword: ENGLISH
Theme_Keyword: SPANISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: OCEAN > SOUTHERN OCEAN > SOUTH SHETLAND ISLANDS > POTTER COVE
Place_Keyword: CONTINENT > ANTARCTICA > CALETA POTTER
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: WALTER PATRICIO MACCORMACK
Contact_Position: INVESTIGATOR
Contact_Position: TECHNICAL CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Departamento Ciencias Biologicas
Address: Direccion Nacional del Antartico
Address: Instituto Antartico Argentino
Address: Cerrito 1248
City: Capital Federal
Postal_Code: C1010AAZ
Country: Argentina
Contact_Voice_Telephone: 54-11-4816-5734
Contact_Facsimile_Telephone: 54-11-4812-2039
Contact_Electronic_Mail_Address: wmac@huemul.ffyb.uba.ar
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Not Available
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: AR/CDA > Centro de Datos Antarticos, Argentina
Contact_Person: CELIA DIEGO ELBA RAFAEL IZQUIERDO GOMEZ IZQUIERDO
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Centro de Datos Antarticos
Address: Direccion Nacional del Antartico
Address: Instituto Antartico Argentino
Address: Cerrito 1248
City: Capital Federal
Postal_Code: C1010AAZ
Country: ARGENTINA
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Centro de Datos Antarticos
Address: Direccion Nacional del Antartico
Address: Instituto Antartico Argentino
Address: Cerrito 1248
City: Capital Federal
Postal_Code: C1010AAZ
Country: ARGENTINA
Contact_Voice_Telephone: 54-11-4812-2086
Contact_Voice_Telephone: + 54 11 4815 4064
Contact_Facsimile_Telephone: 54-11-4812-2086
Contact_Facsimile_Telephone: + 54 11 4812 2086
Contact_Electronic_Mail_Address: celeiz_antar@hotmail.com
Contact_Electronic_Mail_Address: celeiz@dna.gov.ar
Contact_Electronic_Mail_Address: cda@dna.gov.ar
Contact_Electronic_Mail_Address: diegogi@dna.gov.ar
Resource_Description: CDA_AR_PICTA-10-2004-EFFECTS-UV
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.dna.gov.ar/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20050614
Metadata_Review_Date: 20051103
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CELIA ELBA IZQUIERDO
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Centro de Datos Antarticos
Address: Direccion Nacional del Antartico
Address: Instituto Antartico Argentino
Address: Cerrito 1248
City: Capital Federal
Postal_Code: C1010AAZ
Country: ARGENTINA
Contact_Voice_Telephone: 54-11-4812-2086
Contact_Facsimile_Telephone: 54-11-4812-2086
Contact_Electronic_Mail_Address: celeiz_antar@hotmail.com
Contact_Electronic_Mail_Address: celeiz@dna.gov.ar
Contact_Electronic_Mail_Address: cda@dna.gov.ar
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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