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Investigation into the environmental factors that drive microbial diversity in the soils of the Miers Valley, Mt Erebus, Bratina Island and Beacon Valley

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: Investigation into the environmental factors that drive microbial diversity in the soils of the Miers Valley, Mt Erebus, Bratina Island and Beacon Valley
Description:
Abstract:
In this study, the active microbial component in the Dry Valleys system was characterized using newly developed molecular techniques with the aid of in situ augmentation and cloches (wind protection) which dampen the effects of the extreme weather conditions unique to the ecosystem. The aim was to ascertain those bacteria that are truly functional and to understand the environmental factors driving the incredible microbial diversity in this system. 152 mineral soil samples and 145 hypolith samples were collected from the Miers Valley. Two mummified seal tissue samples were also collected. Mineral soil samples were collected from Battleship Promontory and from the Upper Wright Valley. Some rock samples were also collected from Battleship Promontory, Marble Point and the Upper Wright Valley. Soil samples were also used for in situ augmentation and stable isotope probing (SIP) experiments in conjunction with cloches. Specific experiments include: 1) Microbial biomass was determined by in situ ATP analysis. 2) The drivers for microbial diversity were identified from collected soil samples subjected to in situ incubation after augmentation of carbon (glucose), nitrogen (NH4Cl), water (deionized and sterile) or combinations of these potential drivers. ATP levels and CO2 flux were measured for augmented samples over time to monitor the effect of those augmentations on overall biomass and activity and then analysed further using molecular techniques. 3) SIP analysis was used to augment microbial communities in soil, followed by extraction of total DNA. Organisms that are alive and active and respond to the addition of the isotope incorporate it into their DNA. 4) Cloches were used to trap heat from solar radiation and provide shelter from cooling winds. In situ soil augmentation and SIP experiments were placed in cloches to dampen the effects of extreme weather on the samples and to maximize solar gain. 5) DNA and RNA were extracted to provide zero-time controls. 6) The quality of water present in the soil was determined by measuring water activity and water potential. 7) The flux of CO2 out of the augmented (under seal or hypolith) soil compared to an unaugmented control was measured. 8) Replicate hypolith samples of structurally different classes were sampled and subsequently weighed for carbon and nitrogen analysis. Data loggers measured temperature, soil humidity and incident light over a 7 day period. Sensors were deployed for long term measurments. Collected samples (comprising DNA extracts, soil samples, mummified seal tissue samples, RNALater-stabilised soil samples, rock samples, hypolith samples) were returned to the University of Waikato.
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Cowan, D.A. Ah Tow, L. Smith, J.J. Cary, C. Moodley, K. 2005 Microbial molecular ecology of continental Antarctic biotopes. Antarctica: Global Laboratory for Scientific and International Collaboration. Tan, A., Yasin, Z., Mansor, M.(eds), Kuala Lumpur Academy of Sciences Malaysia Adams, B.J. Bardgett, R.D. Ayres, E. Wall, D.H. Aislabie, J. Bamforth, S. Bargagli, R. Cary, C. Cavacini, P. Connell, L. Convey, P. Fell, J.W. Frati, F. Hogg, I. Newsham, K.K. O’Donnell, A. Russell, N. Seppelt, R.D. Stevens, M.I. 2006 Diversity and distribution of Victoria Land biota. Soil biology and biochemistry 38(10): 3003-3018 doi:10.1016/j.soilbio.2006.04.030 Barrett, J.E. Virginia, R.A. Wall, D.H. Cary, S.C. Adams, B.J. Hacker, A.L. Aislabie, J.M. 2006 Co-variation in soil biodiversity and biogeochemistry in northern and southern Victoria Land, Antarctica. Antarctic science 18(4): 535-548 doi: 10.1017/S0954102006000587 Hogg, I.D. Cary, S.C. Convey, P. Newsham, K.K. O’Donnell, A.G. Adams, B.J. Aislabie, J. Frati, F. Stevens, M.I. Wall, D.H. 2006 Biotic interactions in Antarctic terrestrial ecosystems: Are they a factor? Soil biology and biochemistry 38(10): 3035-3040 doi:10.1016/j.soilbio.2006.04.026 Smith, J.J. Ah Tow, L. Baker, G. Cary, C. Cowan, D.A. 2006 Bacterial diversity in three different Antarctic cold desert mineral soils. Microbial ecology 51(4): 413-421 Cowan, D.A. Casanueva, A. Stafford, W. 2007 Ecology and biodiversity of cold-adapted organisms. In: Physiology and biochemistry of extremophiles. Gerday, C., Glansdorff, N.(eds), Washington, DC. ASM Press Cowan, D.A. Casanueva, A. Stafford, W. 2007 Ecology and biodiversity of cold-adapted organisms. In: Physiology and biochemistry of extremophiles. Gerday, C., Glansdorff, N.(eds), Washington, DC. ASM Press Niederberger, T.D. McDonald, I.R. Hacker, A.L. Soo, R.M. Barrett, J.E. Wall, D.H. Cary, C. 2008 Microbial community composition in soils of Northern Victoria Land, Antarctica Environmental microbiology 10(7): 1713-1624 doi:10.1111/j.1462-2920.2008.01593.x Wood, S.A. Rueckert, A. Cowan, D.A. Cary, S.C. 2008 Sources of edaphic cyanobacterial diversity in the Dry Valleys of Eastern Antarctica. The ISME journal 2: 308-320 doi:10.1038/ismej.2007.104
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20080102
Ending_Date: 20080222
Currentness_Reference:Unknown
Status:
Progress: In Work
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 163.812
East_Bounding_Coordinate: 163.812
North_Bounding_Coordinate: -78.0943
South_Bounding_Coordinate: -78.0943
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 160.7636
East_Bounding_Coordinate: 160.7636
North_Bounding_Coordinate: -77.5188
South_Bounding_Coordinate: -77.5188
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 160.9113
East_Bounding_Coordinate: 160.9113
North_Bounding_Coordinate: -76.9116
South_Bounding_Coordinate: -76.9116
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: GCMD INSTRUMENT
Theme_Keyword_Thesaurus: PROJECT
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > LAND SURFACE > SOILS > MICRONUTRIENTS/TRACE ELEMENTS
Theme_Keyword: EARTH SCIENCE > LAND SURFACE > SOILS > SOIL MOISTURE/WATER CONTENT
Theme_Keyword: EARTH SCIENCE > LAND SURFACE > SOILS > SOIL PRODUCTIVITY
Theme_Keyword: EARTH SCIENCE > LAND SURFACE > SOILS > SOIL TEMPERATURE
Theme_Keyword: EARTH SCIENCE > LAND SURFACE > SOILS > MICROFLORA
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > BIODIVERSITY FUNCTIONS
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > COMMUNITY STRUCTURE
Theme_Keyword: FIELD SURVEYS
Theme_Keyword: LABORATORY
Theme_Keyword: SOIL MOISTURE PROBE
Theme_Keyword: SOIL SAMPLER
Theme_Keyword: SOIL TEMPERATURE PROBE
Theme_Keyword: LTER > LONG-TERM ECOLOGICAL RESEARCH
Theme_Keyword: LGP > LATITUDINAL GRADIENT PROJECT
Theme_Keyword: IPY > INTERNATIONAL POLAR YEAR
Theme_Keyword: EBA > EVOLUTION AND BIODIVERSITY IN THE ANTARCTIC
Theme_Keyword: BIOTA
Theme_Keyword: ENVIRONMENT
Theme_Keyword: GEOSCIENTIFIC INFORMATION
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: CONTINENT > ANTARCTICA > BATTLESHIP PROMONTORY
Place_Keyword: CONTINENT > ANTARCTICA > MIERS VALLEY
Place_Keyword: CONTINENT > ANTARCTICA > WRIGHT VALLEY
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CRAIG CARY
Contact_Person_Primary:
Contact_Person: DON COWAN
Contact_Position: INVESTIGATOR
Contact_Position: INVESTIGATOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Biological Sciences
Address: University of Waikato
Address: PO BOX 3105
City: Hamilton
Postal_Code: 3105
Country: New Zealand
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Department of Biotechnology
Address: University of the Western Cape
Address: Bellville
City: Cape Town
Postal_Code: 7535
Country: SOUTH AFRICA
Contact_Voice_Telephone: +64 7 838 4593
Contact_Voice_Telephone: +27 (21) 959 2083
Contact_Facsimile_Telephone: +64 7 838 4218
Contact_Facsimile_Telephone: +27 (21) 959 3505
Contact_Electronic_Mail_Address: caryc@UDel.Edu
Contact_Electronic_Mail_Address: c.cary@waikato.ac.nz
Contact_Electronic_Mail_Address: dcowan@uwc.ac.za
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
Rocks and sand were collected from the Miers Valley, Upper Wright Valley and Battleship Promontory. All of the collected samples are stored at the University of Waikato, Hamilton New Zealand in a PC2 containment facility. Various amounts of the samples are held at the University of Western Cape, Cape Town, South Africa. The samples are stored at -80C. The samples were used for DNA analysis and this analysis uses very little material. Therefore, most if not all, of the samples still exist. Please contact the investigator for more information.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Resource_Description: K023_2007_2008_NZ_1
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.antarcticanz.govt.nz/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://sci.waikato.ac.nz/staff/biol/carychttp://ww...
Access_Instructions:
The investigators homepage
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20090430
Metadata_Review_Date: 20090702
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: CEISHA POIROT
Contact_Person_Primary:
Contact_Person: SHULAMIT GORDON
Contact_Position: DIF AUTHOR
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: c.poirot@antarcticanz.govt.nz
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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