The bacterial biomass of sea ice from Seabees Hook, Cape Hallett region

Federal Geographic Data Committee (FGDC) Metadata:

• Identification_Information
• Data_Quality_Information
• Spatial_Reference_Information
• Distribution_Information
• Metadata_Reference_Information

Identification_Information:

Citation:

Citation_Information:

Originator: Unknown

Publication_Date: Unknown

Title: The bacterial biomass of sea ice from Seabees Hook, Cape Hallett region

Description:

Abstract:

A taxonomic assessment of sea ice bacteria abundance and their response to climate induced stress was investigated from the sea ice around Seabee Hook, Cape Hallett. Four sites at different points around Seabee Hook were established for a representative survey of the annual ice present in Edisto Inlet. The ice had broken out the previous year and new ice formed over the winter providing ideal conditions to compare the distribution and abundance of bacteria occurring in pack ice at the tongue of the Mertz Glacier with bacteria from fast ice at Cape Hallett. Bacteria were collected from the ice at regular intervals with ice cores. A complete ice profile was extracted at each site to examine community composition and structure. Water samples were also collected from 5, 25 and 50m depths and processed in the same way as ice cores and compared. In the lab, bacterial samples were grown under light and dark conditions. Epi-fluorescence microscopy was used to analyse the metabolic activity of bacterial cells by CTC staining (which reveals metabolically active cells), DAPI staining (total cell numbers), VSP (Vital Stain and Probe which utilises oligonucleotide probes to distinguish metabolic activity), DVC (Direct Viable Count - which indicates whether cells are capable of undergoing division) and VIAGRAM (which distinguishes between live and dead cells and also gram positive and gram negative cells). The results were examined using laser based technology (flow cytometry) and epi-fluorescence microscopy. If bacteria do respond to light, one possible explanation is that they respond to increased levels of algal photosynthate. Sugars produced by photosynthesis may be released into the seawater and then be taken up by bacterial cells. To test this idea, 14CO2 was fed to sea ice algae and then they were incubated in the light at -2 °C. The algal cells were removed and the filtrate containing labelled photosynthate was fed to sea ice bacteria. Samples were analysed by a Liquid Scintillation counter.

Purpose:

Not Available

Supplemental_Information:

REFERENCE: Martin, A. 2005. Antarctic bacteria, sea ice ecosystem dynamics and global climate change. MSc thesis, Victoria University of Wellington, New Zealand.

Supplemental_Information:

REFERENCE: Martin AR, Hall J, O’Toole R, Davy SK, Ryan KG, (2008). High single-cell metabolic activity in Antarctic sea ice bacteria. Aquatic Microbial Ecology 52, 25-31.

Supplemental_Information:

REFERENCE: Martin, A. 2009. Microbial loop dynamics in Antarctic sea-ice. PhD Thesis Victoria University of Wellington. pp 177

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 20031119

Ending_Date: 20031216

Currentness_Reference:Unknown

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 20041112

Ending_Date: 20041207

Currentness_Reference:Unknown

Status:

Progress: Complete

Maintenance_and_Update_Frequency: As needed

Spatial_Domain:

Description_of_Geographic_Extent:

Bounding_Coordinates:

West_Bounding_Coordinate: 170.2666

East_Bounding_Coordinate: 170.2666

North_Bounding_Coordinate: -72.3166

South_Bounding_Coordinate: -72.3166

Keywords:

Theme:

Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS

Theme_Keyword_Thesaurus: GCMD PLATFORM

Theme_Keyword_Thesaurus: PROJECT

Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY

Theme_Keyword_Thesaurus: DATA SET LANGUAGE

Theme_Keyword: EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CHLOROPHYLL

Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA

Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > BIODIVERSITY FUNCTIONS

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOMASS DYNAMICS

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION

Theme_Keyword: FIELD SURVEYS

Theme_Keyword: LABORATORY

Theme_Keyword: FIELD INVESTIGATION

Theme_Keyword: LGP > LATITUDINAL GRADIENT PROJECT

Theme_Keyword: EBA > EVOLUTION AND BIODIVERSITY IN THE ANTARCTIC

Theme_Keyword: BIOTA

Theme_Keyword: OCEANS

Theme_Keyword: ENGLISH

Place:

Place_Keyword_Thesaurus: GCMD

Place_Keyword: CONTINENT > ANTARCTICA

Place_Keyword: CONTINENT > ANTARCTICA > CAPE HALLETT

Place_Keyword: CONTINENT > ANTARCTICA > SEABEES HOOK

Place_Keyword: GEOGRAPHIC REGION > POLAR

Access_Constraints: Not Available

Use_Constraints:

Not Available

Point_of_Contact:

Contact_Information:

Contact_Person_Primary:

Contact_Person: KEN RYAN

Contact_Position: INVESTIGATOR

Contact_Address:

Address_Type: Mailing and Physical Address

Address: Victoria University of Wellington

Address: School of Biological Sciences

Address: PO BOX 600

City: Wellington

Postal_Code: 6140

Country: New Zealand

Contact_Voice_Telephone: +64 4 463 6083

Contact_Facsimile_Telephone: +64 4 463 5331

Contact_Electronic_Mail_Address: ken.ryan@vuw.ac.nz

Data_Quality_Information:

Attribute_Accuracy:

Attribute_Accuracy_Report:

The data is contained in the thesis. Existing samples are held at Victoria University of Wellington with a spreadsheet of sample numbers. All raw data is held by the investigator. Please contact Dr Ken Ryan for more information.

Logical_Consistency_Report:

Not Available

Completeness_Report:

Not Available

Lineage:

Process_Step:

Process_Description:

Not Available

Process_Date: Unknown

Spatial_Reference_Information:

Distribution_Information:

Distributor:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand

Contact_Person: SHULAMIT GORDON

Contact_Position: DATA CENTER CONTACT

Contact_Address:

Address_Type: Mailing and Physical Address

Address: Antarctica New Zealand

Address: Private Bag 4745

City: Christchurch

Country: New Zealand

Contact_Voice_Telephone: +64 3 358 0200

Contact_Facsimile_Telephone: +64 3 358 0211

Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz

Resource_Description: K043_2003_2005_NZ_2

Distribution_Liability:

Not Available

Standard_Order_Process:

Digital_Form:

Digital_Transfer_Information:

Format_Name: Not Available

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

http://www.antarcticanz.govt.nz/

Access_Instructions:

DATA CENTER URL

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

http://researcharchive.vuw.ac.nz/handle/10063/133

Access_Instructions:

Andrew Martin MSc pdf

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

http://hdl.handle.net/10063/1101

Access_Instructions:

Andrew Martin PhD pdf

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

http://www.victoria.ac.nz/sbs/staff/staff_research...

Access_Instructions:

Investigators research page

Fees: Not Available

Metadata_Reference_Information:

Metadata_Date: 20050922

Metadata_Review_Date: 20100223

Metadata_Contact:

Contact_Information:

Contact_Person_Primary:

Contact_Person: SHULAMIT GORDON

Contact_Position: DIF AUTHOR

Contact_Address:

Address_Type: Mailing and Physical Address

Address: Antarctica New Zealand

Address: Private Bag 4745

City: Christchurch

Country: New Zealand

Contact_Voice_Telephone: +64 3 358 0200

Contact_Facsimile_Telephone: +64 3 358 0211

Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz

Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata

Metadata_Standard_Version: FGDC-STD-001-1998

Metadata_Time_Convention: local time