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The bacterial biomass of sea ice from Seabees Hook, Cape Hallett region

Federal Geographic Data Committee (FGDC) Metadata:


Identification_Information:
Citation:
Citation_Information:
Originator: Unknown
Publication_Date: Unknown
Title: The bacterial biomass of sea ice from Seabees Hook, Cape Hallett region
Description:
Abstract:
A taxonomic assessment of sea ice bacteria abundance and their response to climate induced stress was investigated from the sea ice around Seabee Hook, Cape Hallett. Four sites at different points around Seabee Hook were established for a representative survey of the annual ice present in Edisto Inlet. The ice had broken out the previous year and new ice formed over the winter providing ideal conditions to compare the distribution and abundance of bacteria occurring in pack ice at the tongue of the Mertz Glacier with bacteria from fast ice at Cape Hallett. Bacteria were collected from the ice at regular intervals with ice cores. A complete ice profile was extracted at each site to examine community composition and structure. Water samples were also collected from 5, 25 and 50m depths and processed in the same way as ice cores and compared. In the lab, bacterial samples were grown under light and dark conditions. Epi-fluorescence microscopy was used to analyse the metabolic activity of bacterial cells by CTC staining (which reveals metabolically active cells), DAPI staining (total cell numbers), VSP (Vital Stain and Probe which utilises oligonucleotide probes to distinguish metabolic activity), DVC (Direct Viable Count - which indicates whether cells are capable of undergoing division) and VIAGRAM (which distinguishes between live and dead cells and also gram positive and gram negative cells). The results were examined using laser based technology (flow cytometry) and epi-fluorescence microscopy. If bacteria do respond to light, one possible explanation is that they respond to increased levels of algal photosynthate. Sugars produced by photosynthesis may be released into the seawater and then be taken up by bacterial cells. To test this idea, 14CO2 was fed to sea ice algae and then they were incubated in the light at -2 °C. The algal cells were removed and the filtrate containing labelled photosynthate was fed to sea ice bacteria. Samples were analysed by a Liquid Scintillation counter.
Purpose:
Not Available
Supplemental_Information:
REFERENCE: Martin, A. 2005. Antarctic bacteria, sea ice ecosystem dynamics and global climate change. MSc thesis, Victoria University of Wellington, New Zealand.
Supplemental_Information:
REFERENCE: Martin AR, Hall J, O’Toole R, Davy SK, Ryan KG, (2008). High single-cell metabolic activity in Antarctic sea ice bacteria. Aquatic Microbial Ecology 52, 25-31.
Supplemental_Information:
REFERENCE: Martin, A. 2009. Microbial loop dynamics in Antarctic sea-ice. PhD Thesis Victoria University of Wellington. pp 177
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20031119
Ending_Date: 20031216
Currentness_Reference:Unknown
Time_Period_of_Content:
Time_Period_Information:
Range_of_Dates/Times:
Beginning_Date: 20041112
Ending_Date: 20041207
Currentness_Reference:Unknown
Status:
Progress: Complete
Maintenance_and_Update_Frequency: As needed
Spatial_Domain:
Description_of_Geographic_Extent:
Bounding_Coordinates:
West_Bounding_Coordinate: 170.2666
East_Bounding_Coordinate: 170.2666
North_Bounding_Coordinate: -72.3166
South_Bounding_Coordinate: -72.3166
Keywords:
Theme:
Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS
Theme_Keyword_Thesaurus: GCMD PLATFORM
Theme_Keyword_Thesaurus: PROJECT
Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY
Theme_Keyword_Thesaurus: DATA SET LANGUAGE
Theme_Keyword: EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CHLOROPHYLL
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > BIODIVERSITY FUNCTIONS
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > BIOMASS DYNAMICS
Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
Theme_Keyword: FIELD SURVEYS
Theme_Keyword: LABORATORY
Theme_Keyword: FIELD INVESTIGATION
Theme_Keyword: LGP > LATITUDINAL GRADIENT PROJECT
Theme_Keyword: EBA > EVOLUTION AND BIODIVERSITY IN THE ANTARCTIC
Theme_Keyword: BIOTA
Theme_Keyword: OCEANS
Theme_Keyword: ENGLISH
Place:
Place_Keyword_Thesaurus: GCMD
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: CONTINENT > ANTARCTICA > CAPE HALLETT
Place_Keyword: CONTINENT > ANTARCTICA > SEABEES HOOK
Place_Keyword: GEOGRAPHIC REGION > POLAR
Access_Constraints: Not Available
Use_Constraints:
Not Available
Point_of_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: KEN RYAN
Contact_Position: INVESTIGATOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Victoria University of Wellington
Address: School of Biological Sciences
Address: PO BOX 600
City: Wellington
Postal_Code: 6140
Country: New Zealand
Contact_Voice_Telephone: +64 4 463 6083
Contact_Facsimile_Telephone: +64 4 463 5331
Contact_Electronic_Mail_Address: ken.ryan@vuw.ac.nz
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Data_Quality_Information:
Attribute_Accuracy:
Attribute_Accuracy_Report:
The data is contained in the thesis. Existing samples are held at Victoria University of Wellington with a spreadsheet of sample numbers. All raw data is held by the investigator. Please contact Dr Ken Ryan for more information.
Logical_Consistency_Report:
Not Available
Completeness_Report:
Not Available
Lineage:
Process_Step:
Process_Description:
Not Available
Process_Date: Unknown
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Spatial_Reference_Information:
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Distribution_Information:
Distributor:
Contact_Information:
Contact_Organization_Primary:
Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand
Contact_Person: SHULAMIT GORDON
Contact_Position: DATA CENTER CONTACT
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Resource_Description: K043_2003_2005_NZ_2
Distribution_Liability:
Not Available
Standard_Order_Process:
Digital_Form:
Digital_Transfer_Information:
Format_Name: Not Available
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.antarcticanz.govt.nz/
Access_Instructions:
DATA CENTER URL
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://researcharchive.vuw.ac.nz/handle/10063/133
Access_Instructions:
Andrew Martin MSc pdf
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://hdl.handle.net/10063/1101
Access_Instructions:
Andrew Martin PhD pdf
Digital_Transfer_Option:
Online_Option:
Computer_Contact_Information:
Network_Address:
Network_Resource_Name:
http://www.victoria.ac.nz/sbs/staff/staff_research...
Access_Instructions:
Investigators research page
Fees: Not Available
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Metadata_Reference_Information:
Metadata_Date: 20050922
Metadata_Review_Date: 20100223
Metadata_Contact:
Contact_Information:
Contact_Person_Primary:
Contact_Person: SHULAMIT GORDON
Contact_Position: DIF AUTHOR
Contact_Address:
Address_Type: Mailing and Physical Address
Address: Antarctica New Zealand
Address: Private Bag 4745
City: Christchurch
Country: New Zealand
Contact_Voice_Telephone: +64 3 358 0200
Contact_Facsimile_Telephone: +64 3 358 0211
Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz
Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata
Metadata_Standard_Version: FGDC-STD-001-1998
Metadata_Time_Convention: local time
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