The response of sea ice and brine water algae and bacteria to changes in salinity, temperature, light and UVB in Terra Nova Bay

Federal Geographic Data Committee (FGDC) Metadata:

• Identification_Information
• Data_Quality_Information
• Spatial_Reference_Information
• Distribution_Information
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Identification_Information:

Citation:

Citation_Information:

Originator: Unknown

Publication_Date: Unknown

Title: The response of sea ice and brine water algae and bacteria to changes in salinity, temperature, light and UVB in Terra Nova Bay

Description:

Abstract:

Samples of the bottom ice algae and brine algae (within 30cm of the top of the ice) were subjected to temperature, salinity, light and UVB experiments and analysed with PAM rapid light curves to determine the response of the algae to changes they will encounter during the processes of ice formation at the start of winter and ice melting in summer. Brine algae are likely to be adapted to high light and high UVB conditions near the surface of the ice and may have produced higher levels of protective pigments, MAA's, therefore the content of MAA's was analysed. Imaging PAM fluorometry was also used to gain a 3D image of photosynthetic stressors during the process of ice freeze and thaw on both algae and bacterial cells. The difference in thylakoid structure with increase in temperature was also analysed. Bacterial cells were collected at the end of exposure and a CTC assay was performed on them to determine the effect of UVB radiation. Bottom ice algae exposed to different light levels and UV exposure was analysed for cell counts, antioxidants and MAAs. Bacterial response to changes in light and salinity was examined by adding a fluorescent stain (CTC) to a melted entire sea ice core, a brine sample and a bottom ice sample and exposing the samples to various combinations of light and salinity. Samples were examined for cells that fluoresce due to metabolized CTC. The bacterial response to light and the presence/absence/inhibition of microalgae was examined using the vital stain and probe technique after exposing melted ice core samples to a range of irradiances for 4 hours. Samples of the brine community were collected and incubated for 5 days to determine the longer term response to light. Tritiated leucine was used calibrate the use of the CTC method.

Purpose:

Not Available

Supplemental_Information:

REFERENCE: Martin, A., Hall, J.A., Ryan, K.G. 2009. Low salinity and high-level UV-B radiation reduce single-cell activity in Antarctic sea ice bacteria. Applied and environmental microbiology 75: 7570-7573 2009. doi:10.1128/AEM.00829-09

Supplemental_Information:

REFERENCE: Martin, A. 2009. Microbial loop dynamics in Antarctic sea-ice. PhD Thesis Victoria University of Wellington. pp 177

Supplemental_Information:

REFERENCE: Ryan, K.G., Cowie, R.O.M, Liggins, E., McNaughtan, D., Martin, A., Davey, S.K. 2009. The short-term effect of irradiance on the photosynthetic properties of Antarctic fast-ice microalgal communities. Journal of phycology. 10.1111/j.1529-8817.2009.00764.x

Supplemental_Information:

REFERENCE: Koh, E. Biodiversity of prokaryotes in Antarctic sea ice. PhD thesis in progress, Victoria University of Wellington, New Zealand.

Supplemental_Information:

REFERENCE: Rajanahally, M. Ecophysiology of Antarctic sea ice algae. . PhD thesis in progress, Victoria University of Wellington, New Zealand.

Supplemental_Information:

REFERENCE: Ryan, K.G., Liggins, E., McNaughtan, D., Martin, A., Tay, M. & Davy S.K. (2008). Imaging PAM visualisation of sea ice algae and their response to environmental change. In: Rudenko, N.; Klepikov, A. (Eds.) SCAR/IASC IPY Open Science Conference “Polar Research - Arctic and Antarctic perspectives in the International Polar Year”: Abstract volume (pp. 264). State Scientific Center of the Russian Federation the Arctic and Antarctic Research Institute, Elektronsdart-Print Joint-Stock Company. ISBN 978-5-98364-013-09

Supplemental_Information:

REFERENCE: Rajanahally, M, Bryant, L. Koh, EY, Davy S., Martin A, Ryan KG (2008) Antarctic sea ice productivity and ecophysiology. NZ Marine Sciences Society Conference Christchurch July 2008.

Supplemental_Information:

REFERENCE: Rajanahally, M, Ryan KG and Davy S (2007). Antarctic microalgae: physiological responses to environmental changes. New Zealand Marine Sciences Society Annual Conference; Hamilton, New Zealand.

Supplemental_Information:

REFERENCE: Cowie, R., Liggins L., McNaughtan D, Martin A, Davy S., and Ryan KG (2008) The effect of irradiance on Antarctic fast-ice microalgal communities Antarctic New Zealand Annual Conference, Dunedin July 2008.

Supplemental_Information:

REFERENCE: Rajanahally, M, Bryant, L. Koh, EY, Davy S., Martin A, Ryan KG (2008). Antarctic sea ice productivity and ecophysiology. Antarctic New Zealand Annual Conference, Dunedin July 2008.

Supplemental_Information:

REFERENCE: Ryan KG, Davy, SK, O’Toole, R, Martin, A, Ralph P (2006) Effects of Salinity and Light on the Microbial Community in Antarctic Sea Ice. SCAR XXIX/ COMNAP XVIII Conference Hobart 2006.

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 20061103

Ending_Date: 20061209

Currentness_Reference:Unknown

Time_Period_of_Content:

Time_Period_Information:

Range_of_Dates/Times:

Beginning_Date: 20071110

Ending_Date: 20071210

Currentness_Reference:Unknown

Status:

Progress: Complete

Maintenance_and_Update_Frequency: As needed

Spatial_Domain:

Description_of_Geographic_Extent:

Bounding_Coordinates:

West_Bounding_Coordinate: 164.5

East_Bounding_Coordinate: 164.5

North_Bounding_Coordinate: -74.8333

South_Bounding_Coordinate: -74.8333

Keywords:

Theme:

Theme_Keyword_Thesaurus: GCMD SCIENCE PARAMETERS

Theme_Keyword_Thesaurus: GCMD PLATFORM

Theme_Keyword_Thesaurus: GCMD INSTRUMENT

Theme_Keyword_Thesaurus: PROJECT

Theme_Keyword_Thesaurus: ISO TOPIC CATEGORY

Theme_Keyword_Thesaurus: DATA SET LANGUAGE

Theme_Keyword: EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CHLOROPHYLL

Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA

Theme_Keyword: EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PLANTS > MICROALGAE

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > AQUATIC ECOSYSTEMS > MARINE HABITAT

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > COMMUNITY DYNAMICS > BIODIVERSITY FUNCTIONS

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Theme_Keyword: EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION

Theme_Keyword: LABORATORY

Theme_Keyword: FIELD INVESTIGATION

Theme_Keyword: PAM > PORTABLE FLUORESCENCE ANALYZERS

Theme_Keyword: LGP > LATITUDINAL GRADIENT PROJECT

Theme_Keyword: EBA > EVOLUTION AND BIODIVERSITY IN THE ANTARCTIC

Theme_Keyword: BIOTA

Theme_Keyword: OCEANS

Theme_Keyword: ENGLISH

Place:

Place_Keyword_Thesaurus: GCMD

Place_Keyword: CONTINENT > ANTARCTICA

Place_Keyword: CONTINENT > ANTARCTICA > TERRA NOVA BAY

Place_Keyword: GEOGRAPHIC REGION > POLAR

Access_Constraints: Not Available

Use_Constraints:

Not Available

Point_of_Contact:

Contact_Information:

Contact_Person_Primary:

Contact_Person: KEN RYAN

Contact_Position: INVESTIGATOR

Contact_Address:

Address_Type: Mailing and Physical Address

Address: Victoria University of Wellington

Address: School of Biological Sciences

Address: PO BOX 600

City: Wellington

Postal_Code: 6140

Country: New Zealand

Contact_Voice_Telephone: +64 4 463 6083

Contact_Facsimile_Telephone: +64 4 463 5331

Contact_Electronic_Mail_Address: ken.ryan@vuw.ac.nz

Data_Quality_Information:

Attribute_Accuracy:

Attribute_Accuracy_Report:

Existing samples are held at Victoria University of Wellington with a spreadsheet of sample numbers. All raw data is held by the investigators. Please contact Dr Ken Ryan for more information.

Logical_Consistency_Report:

Not Available

Completeness_Report:

Not Available

Lineage:

Process_Step:

Process_Description:

Not Available

Process_Date: Unknown

Spatial_Reference_Information:

Distribution_Information:

Distributor:

Contact_Information:

Contact_Organization_Primary:

Contact_Organization: NZ/NZAI/ANZ > Antarctica New Zealand, New Zealand Antarctic Institute, New Zealand

Contact_Person: SHULAMIT GORDON

Contact_Position: DATA CENTER CONTACT

Contact_Address:

Address_Type: Mailing and Physical Address

Address: Antarctica New Zealand

Address: Private Bag 4745

City: Christchurch

Country: New Zealand

Contact_Voice_Telephone: +64 3 358 0200

Contact_Facsimile_Telephone: +64 3 358 0211

Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz

Resource_Description: K043_2006_2008_NZ_4

Distribution_Liability:

Not Available

Standard_Order_Process:

Digital_Form:

Digital_Transfer_Information:

Format_Name: Not Available

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

http://www.antarcticanz.govt.nz/

Access_Instructions:

DATA CENTER URL

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

http://hdl.handle.net/10063/1101

Access_Instructions:

Andrew Martin PhD Thesis pdf

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

www.lgp.aq/

Access_Instructions:

LGP Project Homepage

Digital_Transfer_Option:

Online_Option:

Computer_Contact_Information:

Network_Address:

Network_Resource_Name:

http://www.victoria.ac.nz/sbs/staff/staff_research...

Access_Instructions:

Investigators research page

Fees: Not Available

Metadata_Reference_Information:

Metadata_Date: 20050922

Metadata_Review_Date: 20100223

Metadata_Contact:

Contact_Information:

Contact_Person_Primary:

Contact_Person: SHULAMIT GORDON

Contact_Position: DIF AUTHOR

Contact_Address:

Address_Type: Mailing and Physical Address

Address: Antarctica New Zealand

Address: Private Bag 4745

City: Christchurch

Country: New Zealand

Contact_Voice_Telephone: +64 3 358 0200

Contact_Facsimile_Telephone: +64 3 358 0211

Contact_Electronic_Mail_Address: s.gordon@antarcticanz.govt.nz

Metadata_Standard_Name: FGDC Content Standards for Digital Geospatial Metadata

Metadata_Standard_Version: FGDC-STD-001-1998

Metadata_Time_Convention: local time