Title: Microbial mat development and production variation in relation to physio-chemical variation in 13 ponds that all originated
from one greater pond near Bratina Island on the McMurdo Ice Shelf
Microbial mat development and production varies from pond to pond due to the physio-chemical variation amongst the ponds.
To study this variation a series of 13 ponds were selected that had originated from one greater pond which had drained due
to a tide crack opening up. Cores of microbial mats were collected from all 13 ponds for particulate nutrient and pigment
analyses, biomass determination and species composition. Surface water samples from 13 ponds were also collected for phytoplankton,
chlorophyll a, particulate nutrients and dissolved nutrient analysis. Photosynthesis and nitrogen fixation experiments were
conducted on microbial mats collected from 7 ponds. Conductivity and pH measurements, coupled with gross mat morphology were
used to select the most contrasting ponds. Collected samples were experimented on and the photosynthesis and respiration rates
were determined by measuring the rate of evolution or consumption of oxygen using oxygen electrodes. Samples for dissolved
inorganic carbon uptake were taken and injected into vacuette tubes for analysis. Nitrogen fixation was determined and gas
samples from the nitrogen fixation were then collected for analysis by gas chromatography.
Jungblut, A.D. and Neilan, B.A. 2010. NifH gene diversity and expression in a microbial mat community on the McMurdo Ice Shelf,
Antarctica. Antarctic Science 22(2): 117-122.
Howard-Williams, C. Hawes, I. Ecological processes in Antarctic inland waters: interactions between physical processes and
the nitrogen cycle. Antarctic science 19(2): 205-217, 2007.
Sutherland, D.L. Microbial mat communities in response to recent changes in the physiochemical environment of the meltwater
ponds on the McMurdo Ice Shelf, Antarctica. Polar biology 32:1023-1032 2009. doi: 10.1007/s00300-009-0601-x