Title: Distribution data and samples of non-marine algae for culture and DNA analysis to determine evolution and dispersal of algae
along a latitudinal gradient
Algae populations at different points on a latitudinal gradient within the Ross Sea sector were investigated. At Cape Hallett,
the distribution of the different algal communities were mapped by a survey of the area in 10 m x 10 m squares to encompass
the area studied previously (1963). The maps were scanned to produce and electronic layered map of each community type.Temperature,
pH and electrical conductivity was measured in streams and pools in the Cape Hallett region and from Luther Lake. 24 samples
of algae (eukaryotic and cyanobacteria) were collected from Cape Hallett, and 15 samples (almost entirely cyanobacteria) from
Luther Lake. These samples will be used to obtain unialgal cultures of algae for DNA analysis. In addition, samples were also
received/collected from Bratina Island and the Garwood, Miers and Taylor Valleys. Expected long-term results will be a DNA
fingerprint and sequencing library matching morphological descriptions for these organisms as a starting point for the study
of distribution, dispersal and genetic isolation of common algal taxa along a latitudinal gradient.
Novis, P.M. Smissen, R.D. Two genetic and ecological groups of Nostoc commune in Victoria Land, Antarctica, revealed by AFLP
analysis. Antarctic science 18(4): 573-581, 2006.
Place_Keyword: CONTINENT > ANTARCTICA > MIERS VALLEY
Place_Keyword: CONTINENT > ANTARCTICA > GARWOOD VALLEY
Place_Keyword: CONTINENT > ANTARCTICA > TAYLOR VALLEY
Place_Keyword: CONTINENT > ANTARCTICA > BRATINA ISLAND
Place_Keyword: CONTINENT > ANTARCTICA
Place_Keyword: CONTINENT > ANTARCTICA > LUTHER LAKE
Place_Keyword: GEOGRAPHIC REGION > POLAR
If the samples are wanted to be transeferred from Landcare Research, the receiving lab needs to have the necessary containment
certification (i.e. PC2 containment) and transfers have to be done according to MAF protocol. Supply of material is only
available by the investigators permission and knowledge.
Frozen samples and DNA extractions of Nostoc collections are held in at the Molecular Laboratory and Allan Herbarium at Landcare
Research, New Zealand. he lab has PC2 containment and transfers have to be done according to Ministry of Agriculture and Fisheries
(MAF) protocol. Contact Phil Novis: NovisP@landcareresearch.co.nz for more information.